Complete nucleotide sequence and coding strategy of rice hoja blanca virus RNA4

The complete sequence office hoja blanca virus (RHBV) RNA4 has been determined, based on the sequence of the corresponding eDNA clones. RNA4 consists of 1991 nucleotides with two open reading frames (ORFs). One putative ORF is located in the 5'-proximal region of the viral RNA4; it encodes a protein...

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Main Authors: Ramírez, B.C., Lozano Potes, Iván, Constantino, LM, Haenni, AL
Format: Journal Article
Language:Inglés
Published: Microbiology Society 1993
Subjects:
Online Access:https://hdl.handle.net/10568/43292
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author Ramírez, B.C.
Lozano Potes, Iván
Constantino, LM
Haenni, AL
author_browse Constantino, LM
Haenni, AL
Lozano Potes, Iván
Ramírez, B.C.
author_facet Ramírez, B.C.
Lozano Potes, Iván
Constantino, LM
Haenni, AL
author_sort Ramírez, B.C.
collection Repository of Agricultural Research Outputs (CGSpace)
description The complete sequence office hoja blanca virus (RHBV) RNA4 has been determined, based on the sequence of the corresponding eDNA clones. RNA4 consists of 1991 nucleotides with two open reading frames (ORFs). One putative ORF is located in the 5'-proximal region of the viral RNA4; it encodes a protein of predicted M r 20076 which corresponds to the major non-structural protein that accumulates in RHBV-infected rice plants, and which bears limited sequence identity with the helper component of tobacco vein mottling potyvirus. The other ORF is located in the 5'-proximal region of the viral complementary RNA4 and encodes a protein of predicted M r 32469. Between the two ORFs is an intergenic region of 524 nucleotides, part of which can theoretically adopt a stable stem-loop structure; the 5' and 3' ends can potentially base-pair over 16 nucleotides, producing a pan-handle configuration. These characteristics are in favour of an ambisense coding strategy for RHBV RNA4.
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spelling CGSpace432922024-08-27T10:36:44Z Complete nucleotide sequence and coding strategy of rice hoja blanca virus RNA4 Ramírez, B.C. Lozano Potes, Iván Constantino, LM Haenni, AL oryza sativa rice tagosodes orizicolus nucleotide sequence arroz secuencia nucleotídica The complete sequence office hoja blanca virus (RHBV) RNA4 has been determined, based on the sequence of the corresponding eDNA clones. RNA4 consists of 1991 nucleotides with two open reading frames (ORFs). One putative ORF is located in the 5'-proximal region of the viral RNA4; it encodes a protein of predicted M r 20076 which corresponds to the major non-structural protein that accumulates in RHBV-infected rice plants, and which bears limited sequence identity with the helper component of tobacco vein mottling potyvirus. The other ORF is located in the 5'-proximal region of the viral complementary RNA4 and encodes a protein of predicted M r 32469. Between the two ORFs is an intergenic region of 524 nucleotides, part of which can theoretically adopt a stable stem-loop structure; the 5' and 3' ends can potentially base-pair over 16 nucleotides, producing a pan-handle configuration. These characteristics are in favour of an ambisense coding strategy for RHBV RNA4. 1993-11-01 2014-09-24T08:41:55Z 2014-09-24T08:41:55Z Journal Article https://hdl.handle.net/10568/43292 en Open Access Microbiology Society
spellingShingle oryza sativa
rice
tagosodes orizicolus
nucleotide sequence
arroz
secuencia nucleotídica
Ramírez, B.C.
Lozano Potes, Iván
Constantino, LM
Haenni, AL
Complete nucleotide sequence and coding strategy of rice hoja blanca virus RNA4
title Complete nucleotide sequence and coding strategy of rice hoja blanca virus RNA4
title_full Complete nucleotide sequence and coding strategy of rice hoja blanca virus RNA4
title_fullStr Complete nucleotide sequence and coding strategy of rice hoja blanca virus RNA4
title_full_unstemmed Complete nucleotide sequence and coding strategy of rice hoja blanca virus RNA4
title_short Complete nucleotide sequence and coding strategy of rice hoja blanca virus RNA4
title_sort complete nucleotide sequence and coding strategy of rice hoja blanca virus rna4
topic oryza sativa
rice
tagosodes orizicolus
nucleotide sequence
arroz
secuencia nucleotídica
url https://hdl.handle.net/10568/43292
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