Combined mapping of DALP and AFLP markers in cultivated sunflower using F9 recombinant inbred lines
A genetic map was constructed with specific PCRs, DALPs and AFLPs using F8-generation sunflower recombinant inbred lines. RI lines generated from a F2 population of one cross between the two cultivated inbred lines HA89 (maintainer for Pet1 CMS) and LR4 (restorer for Pet1 CMS) were used. A total of...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2003
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/43273 |
| _version_ | 1855543208693465088 |
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| author | Langar, K Lorieux, Mathias Desmarais, E Griveau, Y Gentzbittel, I Berville, A |
| author_browse | Berville, A Desmarais, E Gentzbittel, I Griveau, Y Langar, K Lorieux, Mathias |
| author_facet | Langar, K Lorieux, Mathias Desmarais, E Griveau, Y Gentzbittel, I Berville, A |
| author_sort | Langar, K |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A genetic map was constructed with specific PCRs, DALPs and AFLPs using F8-generation sunflower recombinant inbred lines. RI lines generated from a F2 population of one cross between the two cultivated inbred lines HA89 (maintainer for Pet1 CMS) and LR4 (restorer for Pet1 CMS) were used. A total of 305 markers were located using seven sPCR, 64 DALP and 301 AFLP loci. They were generated with one, seven and 14 primer pairs, respectively. The map construction consisted of a two-step strategy using 6 and 3.1 LOD scores revealed by a simulation file. Mapped markers were assembled into 18 linkage groups covering 2,168.6 cM with an average of 6.1 cM. The distribution of DALPs and AFLPs revealed that both markers tagged different regions to enable covering most of the sunflower genome. This leads to the longest map published so far for sunflower. |
| format | Journal Article |
| id | CGSpace43273 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2003 |
| publishDateRange | 2003 |
| publishDateSort | 2003 |
| record_format | dspace |
| spelling | CGSpace432732021-10-09T07:24:11Z Combined mapping of DALP and AFLP markers in cultivated sunflower using F9 recombinant inbred lines Langar, K Lorieux, Mathias Desmarais, E Griveau, Y Gentzbittel, I Berville, A helianthus annuus genetic maps genetic markers pcr mapas genéticos marcadores genéticos A genetic map was constructed with specific PCRs, DALPs and AFLPs using F8-generation sunflower recombinant inbred lines. RI lines generated from a F2 population of one cross between the two cultivated inbred lines HA89 (maintainer for Pet1 CMS) and LR4 (restorer for Pet1 CMS) were used. A total of 305 markers were located using seven sPCR, 64 DALP and 301 AFLP loci. They were generated with one, seven and 14 primer pairs, respectively. The map construction consisted of a two-step strategy using 6 and 3.1 LOD scores revealed by a simulation file. Mapped markers were assembled into 18 linkage groups covering 2,168.6 cM with an average of 6.1 cM. The distribution of DALPs and AFLPs revealed that both markers tagged different regions to enable covering most of the sunflower genome. This leads to the longest map published so far for sunflower. 2003 2014-09-24T08:41:53Z 2014-09-24T08:41:53Z Journal Article https://hdl.handle.net/10568/43273 en Limited Access |
| spellingShingle | helianthus annuus genetic maps genetic markers pcr mapas genéticos marcadores genéticos Langar, K Lorieux, Mathias Desmarais, E Griveau, Y Gentzbittel, I Berville, A Combined mapping of DALP and AFLP markers in cultivated sunflower using F9 recombinant inbred lines |
| title | Combined mapping of DALP and AFLP markers in cultivated sunflower using F9 recombinant inbred lines |
| title_full | Combined mapping of DALP and AFLP markers in cultivated sunflower using F9 recombinant inbred lines |
| title_fullStr | Combined mapping of DALP and AFLP markers in cultivated sunflower using F9 recombinant inbred lines |
| title_full_unstemmed | Combined mapping of DALP and AFLP markers in cultivated sunflower using F9 recombinant inbred lines |
| title_short | Combined mapping of DALP and AFLP markers in cultivated sunflower using F9 recombinant inbred lines |
| title_sort | combined mapping of dalp and aflp markers in cultivated sunflower using f9 recombinant inbred lines |
| topic | helianthus annuus genetic maps genetic markers pcr mapas genéticos marcadores genéticos |
| url | https://hdl.handle.net/10568/43273 |
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