Characterization of cassava common mosaic virus and a defective RNA species
The genome of cassava common mosaic potexvirus (CsCMV) has been sequenced from cDNA clones and consists of 6376 nucleotides (nt). A 76 nt untranslated region (UTR) at the 5? terminus was followed by ORF1 which potentially encodes a protein of 1449 amino acids (aa). ORFs 2, 3, and 4 were predicted to...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Microbiology Society
1996
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/43258 |
| _version_ | 1855532812286820352 |
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| author | Calvert, Lee A. Cuervo Ibáñez, Maritza Ospina, M.D. Fauquet, Claude M. Ramírez, B.C. |
| author_browse | Calvert, Lee A. Cuervo Ibáñez, Maritza Fauquet, Claude M. Ospina, M.D. Ramírez, B.C. |
| author_facet | Calvert, Lee A. Cuervo Ibáñez, Maritza Ospina, M.D. Fauquet, Claude M. Ramírez, B.C. |
| author_sort | Calvert, Lee A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The genome of cassava common mosaic potexvirus (CsCMV) has been sequenced from cDNA clones and consists of 6376 nucleotides (nt). A 76 nt untranslated region (UTR) at the 5? terminus was followed by ORF1 which potentially encodes a protein of 1449 amino acids (aa). ORFs 2, 3, and 4 were predicted to encode proteins of 231, 112 and 97 aa, respectively. ORF5 potentially encodes a 229 aa protein of 25 kDa that is similar to the coat proteins of other potexviruses. The 3?-terminal UTR of 114 nt was followed by a poly(A) tail. The genomic organization of the CsCMV genome is similar to that of other potexviruses. A cDNA clone that was apparently obtained from a defective RNA species contained both the 5? and 3? UTRs and an ORF that potentially encodes the first 263 aa of ORF1 and the last 33 aa of the coat protein. Defective RNA species were found both in purified preparations of the virus and in total nucleic acid isolated from CsCMV-infected plants. |
| format | Journal Article |
| id | CGSpace43258 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1996 |
| publishDateRange | 1996 |
| publishDateSort | 1996 |
| publisher | Microbiology Society |
| publisherStr | Microbiology Society |
| record_format | dspace |
| spelling | CGSpace432582024-08-27T10:36:36Z Characterization of cassava common mosaic virus and a defective RNA species Calvert, Lee A. Cuervo Ibáñez, Maritza Ospina, M.D. Fauquet, Claude M. Ramírez, B.C. manihot esculenta african cassava mosaic virus nucleotide sequence rna virus del mosaico de la casava secuencia nucleotídica arn The genome of cassava common mosaic potexvirus (CsCMV) has been sequenced from cDNA clones and consists of 6376 nucleotides (nt). A 76 nt untranslated region (UTR) at the 5? terminus was followed by ORF1 which potentially encodes a protein of 1449 amino acids (aa). ORFs 2, 3, and 4 were predicted to encode proteins of 231, 112 and 97 aa, respectively. ORF5 potentially encodes a 229 aa protein of 25 kDa that is similar to the coat proteins of other potexviruses. The 3?-terminal UTR of 114 nt was followed by a poly(A) tail. The genomic organization of the CsCMV genome is similar to that of other potexviruses. A cDNA clone that was apparently obtained from a defective RNA species contained both the 5? and 3? UTRs and an ORF that potentially encodes the first 263 aa of ORF1 and the last 33 aa of the coat protein. Defective RNA species were found both in purified preparations of the virus and in total nucleic acid isolated from CsCMV-infected plants. 1996-03-01 2014-09-24T08:41:51Z 2014-09-24T08:41:51Z Journal Article https://hdl.handle.net/10568/43258 en Open Access Microbiology Society |
| spellingShingle | manihot esculenta african cassava mosaic virus nucleotide sequence rna virus del mosaico de la casava secuencia nucleotídica arn Calvert, Lee A. Cuervo Ibáñez, Maritza Ospina, M.D. Fauquet, Claude M. Ramírez, B.C. Characterization of cassava common mosaic virus and a defective RNA species |
| title | Characterization of cassava common mosaic virus and a defective RNA species |
| title_full | Characterization of cassava common mosaic virus and a defective RNA species |
| title_fullStr | Characterization of cassava common mosaic virus and a defective RNA species |
| title_full_unstemmed | Characterization of cassava common mosaic virus and a defective RNA species |
| title_short | Characterization of cassava common mosaic virus and a defective RNA species |
| title_sort | characterization of cassava common mosaic virus and a defective rna species |
| topic | manihot esculenta african cassava mosaic virus nucleotide sequence rna virus del mosaico de la casava secuencia nucleotídica arn |
| url | https://hdl.handle.net/10568/43258 |
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