Capped nonviral sequences at the 5' end of the mRNAs of Rice Hoja Blanca Virus RNA4
Subgenomic RNAs of both polarities corresponding to rice hoja blanca virus (RHBV) ambisense RNA4 were detected in RHBV-infected rice tissues. Total RNA extracted from RHBV-infected and noninfected rice tissues and RNA4 purified from RHBV ribonucleoprotein particles were used as templates for primer...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
1995
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/43242 |
| _version_ | 1855531655961247744 |
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| author | Ramírez, B.C. Garcin, D Calvert, Lee A. Kolakofsky, D Haenni, AL |
| author_browse | Calvert, Lee A. Garcin, D Haenni, AL Kolakofsky, D Ramírez, B.C. |
| author_facet | Ramírez, B.C. Garcin, D Calvert, Lee A. Kolakofsky, D Haenni, AL |
| author_sort | Ramírez, B.C. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Subgenomic RNAs of both polarities corresponding to rice hoja blanca virus (RHBV) ambisense RNA4 were detected in RHBV-infected rice tissues. Total RNA extracted from RHBV-infected and noninfected rice tissues and RNA4 purified from RHBV ribonucleoprotein particles were used as templates for primer extension studies. The RNAs extracted from RHBV-infected tissues contain a population of RNA molecules with 10 to 17 nonviral nucleotides at their 5' end. The RNA-cDNA hybrids resulting from primer extension of such RNA molecules were specifically immunoselected with anti-cap antibodies, indicating that the subgenomic RNAs are capped and probably serve as mRNAs and that the additional nucleotides at their 5' end possibly derive from host mRNAs via a cap-snatching mechanism. |
| format | Journal Article |
| id | CGSpace43242 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| record_format | dspace |
| spelling | CGSpace432422021-10-09T05:55:41Z Capped nonviral sequences at the 5' end of the mRNAs of Rice Hoja Blanca Virus RNA4 Ramírez, B.C. Garcin, D Calvert, Lee A. Kolakofsky, D Haenni, AL oryza sativa plant viruses virology virus de las plantas virología Subgenomic RNAs of both polarities corresponding to rice hoja blanca virus (RHBV) ambisense RNA4 were detected in RHBV-infected rice tissues. Total RNA extracted from RHBV-infected and noninfected rice tissues and RNA4 purified from RHBV ribonucleoprotein particles were used as templates for primer extension studies. The RNAs extracted from RHBV-infected tissues contain a population of RNA molecules with 10 to 17 nonviral nucleotides at their 5' end. The RNA-cDNA hybrids resulting from primer extension of such RNA molecules were specifically immunoselected with anti-cap antibodies, indicating that the subgenomic RNAs are capped and probably serve as mRNAs and that the additional nucleotides at their 5' end possibly derive from host mRNAs via a cap-snatching mechanism. 1995 2014-09-24T08:41:50Z 2014-09-24T08:41:50Z Journal Article https://hdl.handle.net/10568/43242 en Open Access |
| spellingShingle | oryza sativa plant viruses virology virus de las plantas virología Ramírez, B.C. Garcin, D Calvert, Lee A. Kolakofsky, D Haenni, AL Capped nonviral sequences at the 5' end of the mRNAs of Rice Hoja Blanca Virus RNA4 |
| title | Capped nonviral sequences at the 5' end of the mRNAs of Rice Hoja Blanca Virus RNA4 |
| title_full | Capped nonviral sequences at the 5' end of the mRNAs of Rice Hoja Blanca Virus RNA4 |
| title_fullStr | Capped nonviral sequences at the 5' end of the mRNAs of Rice Hoja Blanca Virus RNA4 |
| title_full_unstemmed | Capped nonviral sequences at the 5' end of the mRNAs of Rice Hoja Blanca Virus RNA4 |
| title_short | Capped nonviral sequences at the 5' end of the mRNAs of Rice Hoja Blanca Virus RNA4 |
| title_sort | capped nonviral sequences at the 5 end of the mrnas of rice hoja blanca virus rna4 |
| topic | oryza sativa plant viruses virology virus de las plantas virología |
| url | https://hdl.handle.net/10568/43242 |
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