Association of spiral filamentous viruslike particles with rice hoja blanca
Fine, spiral, filamentous particles ~3 nm in diameter were consistently associated with hoja blanca-affected rice plants. The filamentous particles were isolated from infected plants following organic solvent clarification, concentration with polyethylene glycol, and equilibrium centrifugation in Cs...
| Autores principales: | , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Scientific Societies
1983
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/43206 |
| _version_ | 1855515740327641088 |
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| author | Morales, Francisco José Niessen, AI |
| author_browse | Morales, Francisco José Niessen, AI |
| author_facet | Morales, Francisco José Niessen, AI |
| author_sort | Morales, Francisco José |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Fine, spiral, filamentous particles ~3 nm in diameter were consistently associated with hoja blanca-affected rice plants. The filamentous particles were isolated from infected plants following organic solvent clarification, concentration with polyethylene glycol, and equilibrium centrifugation in Cs2SO4 or CsCl gradients. Purified preparations exhibited typical nucleoprotein ultraviolet absorption spectra with a maximum at A260 nm and an A260/280 nm ratio of 1.4. The preparations contained one major and one minor protein of molecular weights 34 and 17.5 x 103 daltons, respectively, as determined by SDS-PAGE. In double immunodiffusion tests, an antiserum (prepared by injecting rabbits with purified preparations) produced specific precipitin reactions with leaf extracts of hoja blanca-affected rice plants, but not with leaf extracts of healthy rice plants. No serological reaction was observed in similar tests of leaf extracts from hoja blanca-infected Echinochloa colonum. |
| format | Journal Article |
| id | CGSpace43206 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1983 |
| publishDateRange | 1983 |
| publishDateSort | 1983 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace432062024-04-25T06:00:21Z Association of spiral filamentous viruslike particles with rice hoja blanca Morales, Francisco José Niessen, AI oryza sativa rice tagosodes orizicolus viruses arroz virus Fine, spiral, filamentous particles ~3 nm in diameter were consistently associated with hoja blanca-affected rice plants. The filamentous particles were isolated from infected plants following organic solvent clarification, concentration with polyethylene glycol, and equilibrium centrifugation in Cs2SO4 or CsCl gradients. Purified preparations exhibited typical nucleoprotein ultraviolet absorption spectra with a maximum at A260 nm and an A260/280 nm ratio of 1.4. The preparations contained one major and one minor protein of molecular weights 34 and 17.5 x 103 daltons, respectively, as determined by SDS-PAGE. In double immunodiffusion tests, an antiserum (prepared by injecting rabbits with purified preparations) produced specific precipitin reactions with leaf extracts of hoja blanca-affected rice plants, but not with leaf extracts of healthy rice plants. No serological reaction was observed in similar tests of leaf extracts from hoja blanca-infected Echinochloa colonum. 1983 2014-09-24T08:41:47Z 2014-09-24T08:41:47Z Journal Article https://hdl.handle.net/10568/43206 en Open Access Scientific Societies |
| spellingShingle | oryza sativa rice tagosodes orizicolus viruses arroz virus Morales, Francisco José Niessen, AI Association of spiral filamentous viruslike particles with rice hoja blanca |
| title | Association of spiral filamentous viruslike particles with rice hoja blanca |
| title_full | Association of spiral filamentous viruslike particles with rice hoja blanca |
| title_fullStr | Association of spiral filamentous viruslike particles with rice hoja blanca |
| title_full_unstemmed | Association of spiral filamentous viruslike particles with rice hoja blanca |
| title_short | Association of spiral filamentous viruslike particles with rice hoja blanca |
| title_sort | association of spiral filamentous viruslike particles with rice hoja blanca |
| topic | oryza sativa rice tagosodes orizicolus viruses arroz virus |
| url | https://hdl.handle.net/10568/43206 |
| work_keys_str_mv | AT moralesfranciscojose associationofspiralfilamentousviruslikeparticleswithricehojablanca AT niessenai associationofspiralfilamentousviruslikeparticleswithricehojablanca |