Applying biotechnology tools to improve control diseases of some tropical crops
The application of biotechnology tools has made it possible to make significant advances in the detection of pathogens and in the study of the genetic variability of pathogen populations. As a result, adequate disease management strategies can be planned, and disease resistance markers for plants as...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2004
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| Online Access: | https://hdl.handle.net/10568/43192 |
| _version_ | 1855539346907594752 |
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| author | Álvarez, E. Llano Rodríguez, Germán Alberto Loke, John Bernard Mejía de los Ríos, J.F. |
| author_browse | Llano Rodríguez, Germán Alberto Loke, John Bernard Mejía de los Ríos, J.F. Álvarez, E. |
| author_facet | Álvarez, E. Llano Rodríguez, Germán Alberto Loke, John Bernard Mejía de los Ríos, J.F. |
| author_sort | Álvarez, E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The application of biotechnology tools has made it possible to make significant advances in the detection of pathogens and in the study of the genetic variability of pathogen populations. As a result, adequate disease management strategies can be planned, and disease resistance markers for plants as well as DNA regions associated with resistance can be identified. Diseases, mainly those caused by microorganisms that cannot be cultivated in artificial media such as phytoplasmas, can be diagnosed using PCR. The bacterium Xanthomonas axonopodis pv. manihotis (Xam) can be identified using a specific probe and the identification of Ralstonia solanacearum in the soil is also facilitated by PCR. Species of Phytophthora can also be identified, phytoplasmas classified, and pathogen diversity established by using PCR-RFLP, thus improving the understanding of action mechanisms and how they co-evolve with the host. Other techniques such as RAPD, AFLP, and RAMS have made it possible to study the genetic variability of pathogens such as Sphaceloma manihoticola, Colletotrichum gloeosporioides, C. acutatum, Sphaerotheca pannosa, Ceratocystis paradoxa, and X. axonopodis pv manihotis. The identification of QTL markers associated with resistance to different species of Phytophthora in cassava has helped elucidate the genetics of resistance, whereas the identification of SSR markers associated with resistance to Xam is a tool that facilitates the selection of resistant genotypes. Functional genomics tools such as microarrays will give initial insight on the molecular basis of cassava’s defense response to X. axonopodis pv manihotis. Also, the identification of resistance genes and resistance gene analogs in cassava contributes to the genetic improvement of this crop. Biotechnology is evolving continuously, offering modern tools that help solve plant health problems. |
| format | Journal Article |
| id | CGSpace43192 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2004 |
| publishDateRange | 2004 |
| publishDateSort | 2004 |
| record_format | dspace |
| spelling | CGSpace431922025-11-12T05:59:26Z Applying biotechnology tools to improve control diseases of some tropical crops Álvarez, E. Llano Rodríguez, Germán Alberto Loke, John Bernard Mejía de los Ríos, J.F. plant diseases diagnosis plant biotechnology pathogens pcr rflp enfermedades de las plantas diagnóstico biotecnología vegetal The application of biotechnology tools has made it possible to make significant advances in the detection of pathogens and in the study of the genetic variability of pathogen populations. As a result, adequate disease management strategies can be planned, and disease resistance markers for plants as well as DNA regions associated with resistance can be identified. Diseases, mainly those caused by microorganisms that cannot be cultivated in artificial media such as phytoplasmas, can be diagnosed using PCR. The bacterium Xanthomonas axonopodis pv. manihotis (Xam) can be identified using a specific probe and the identification of Ralstonia solanacearum in the soil is also facilitated by PCR. Species of Phytophthora can also be identified, phytoplasmas classified, and pathogen diversity established by using PCR-RFLP, thus improving the understanding of action mechanisms and how they co-evolve with the host. Other techniques such as RAPD, AFLP, and RAMS have made it possible to study the genetic variability of pathogens such as Sphaceloma manihoticola, Colletotrichum gloeosporioides, C. acutatum, Sphaerotheca pannosa, Ceratocystis paradoxa, and X. axonopodis pv manihotis. The identification of QTL markers associated with resistance to different species of Phytophthora in cassava has helped elucidate the genetics of resistance, whereas the identification of SSR markers associated with resistance to Xam is a tool that facilitates the selection of resistant genotypes. Functional genomics tools such as microarrays will give initial insight on the molecular basis of cassava’s defense response to X. axonopodis pv manihotis. Also, the identification of resistance genes and resistance gene analogs in cassava contributes to the genetic improvement of this crop. Biotechnology is evolving continuously, offering modern tools that help solve plant health problems. La aplicación de las herramientas que ofrece la biotecnología, han permitido lograr grandes avances en la detección de patógenos, adelantar estudios de variabilidad genética de poblaciones de patógenos, lo cual permite planear estrategias adecuadas de manejo de enfermedades, identificar marcadores asociados a la resistencia de las plantas a las enfermedades e identificar regiones de ADN asociadas con resistencia. Mediante la Reacción en Cadena de la Polimerasa (RCP) se pueden diagnosticar enfermedades, principalmente las causadas por microorganismos que no se pueden cultivar fácilmente en medios artificiales, como los fitoplasmas. Mediante una sonda específica es posible identificar la bacteria Xanthomonas axonopodis pv. manihotis. La identificación de Ralstonia solanacearum en suelo se facilita mediante PCR. Mediante PCR-RFLP es posible identificar especies de Phytophthora, clasificar fitoplasmas y establecer la diversidad de patógenos. los cuales permitirán un mejor entendimiento de los mecanismos de acción y su co-evolución con el hospedero. Otras técnicas como RAPD, AFLP y RAMS, han permitido estudiar la variabilidad genética de patógenos como Sphaceloma manihoticola, Colletotrichum gloeosporioides, C. acutatum, Sphaerotheca pannosa, Ceratocystis paradoxa y X. axonopodis pv manihotis. La identificación de marcadores QTLs asociados a la resistencia a diferentes especies de Phytophthora en yuca, han contribuido a dilucidar la genética de la resistencia, mientras que la identificación de marcadores SSR asociados a la resistencia a X. axonopodis pv manihotis es una herramienta que facilita la selección de genotipos resistentes. Herramientas de genómica funcional como los microarreglos permitirán una comprensión de la base molecular de la respuesta de defensa de la yuca a X. axonopodis pv manihotis.. 2004 2014-09-24T08:41:45Z 2014-09-24T08:41:45Z Journal Article https://hdl.handle.net/10568/43192 en Open Access application/pdf |
| spellingShingle | plant diseases diagnosis plant biotechnology pathogens pcr rflp enfermedades de las plantas diagnóstico biotecnología vegetal Álvarez, E. Llano Rodríguez, Germán Alberto Loke, John Bernard Mejía de los Ríos, J.F. Applying biotechnology tools to improve control diseases of some tropical crops |
| title | Applying biotechnology tools to improve control diseases of some tropical crops |
| title_full | Applying biotechnology tools to improve control diseases of some tropical crops |
| title_fullStr | Applying biotechnology tools to improve control diseases of some tropical crops |
| title_full_unstemmed | Applying biotechnology tools to improve control diseases of some tropical crops |
| title_short | Applying biotechnology tools to improve control diseases of some tropical crops |
| title_sort | applying biotechnology tools to improve control diseases of some tropical crops |
| topic | plant diseases diagnosis plant biotechnology pathogens pcr rflp enfermedades de las plantas diagnóstico biotecnología vegetal |
| url | https://hdl.handle.net/10568/43192 |
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