An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia

DNA variation was studied at the intra- andinterspecific levels within the genus Passiflora, with particular emphasis on the subgenusTacsonia and its relationship with the subgenusPassiflora. AFLP fingerprints were generated for35 accessions from 21 species, 14 of which belong to subgenusTacsonia, a...

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Main Authors: Segura, Sergio D., Coppens d'Eeckenbrugge, Geo, Bohórquez Cháux, Adriana, Ollitrault, P, Tohme, Joseph M.
Format: Journal Article
Language:Inglés
Published: Springer 2002
Subjects:
Online Access:https://hdl.handle.net/10568/43172
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author Segura, Sergio D.
Coppens d'Eeckenbrugge, Geo
Bohórquez Cháux, Adriana
Ollitrault, P
Tohme, Joseph M.
author_browse Bohórquez Cháux, Adriana
Coppens d'Eeckenbrugge, Geo
Ollitrault, P
Segura, Sergio D.
Tohme, Joseph M.
author_facet Segura, Sergio D.
Coppens d'Eeckenbrugge, Geo
Bohórquez Cháux, Adriana
Ollitrault, P
Tohme, Joseph M.
author_sort Segura, Sergio D.
collection Repository of Agricultural Research Outputs (CGSpace)
description DNA variation was studied at the intra- andinterspecific levels within the genus Passiflora, with particular emphasis on the subgenusTacsonia and its relationship with the subgenusPassiflora. AFLP fingerprints were generated for35 accessions from 21 species, 14 of which belong to subgenusTacsonia, and four to subgenusPassiflora. The morphologically intermediateP. manicata (subgenusManicata) was also included, as wereP. trinervia (subgenusPsylanthus) and P.alnifolia (subgenus Decaloba). The two latter were used as outgroup species. Twoprimer combinations generated 34–80 scorable fragments pergenotype for a total of 260 fragments. Polymorphism analyses spannedthe levels from individual genotype to section. SubgenusTacsonia presented wide genetic variability atboth intra- and interspecific levels, particularly within wildspecies. The classification based on these markers generally agreeswell with current taxonomic definitions of common species, but lesswith definitions of sections. A geographical component was evident inthe intraspecific variation of P.mixta, P.tripartita var. mollissimaand P. tarminiana, withwider variation in Ecuador and Peru than in Colombia and Venezuela.These same three species, plus P.cumbalensis, formed adjacent clusters, showingcloser affinity with each other than with the other species ofsubgenus Tacsonia. As expected, P. manicata clustered at anintermediate position between subgenera Tacsoniaand Passiflora.
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spelling CGSpace431722024-08-29T11:41:31Z An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia Segura, Sergio D. Coppens d'Eeckenbrugge, Geo Bohórquez Cháux, Adriana Ollitrault, P Tohme, Joseph M. tropical fruits passiflora genetic markers tacsonia genetic variation botany taxonomy frutas tropicales marcadores genéticos variación genética botánica taxonomía DNA variation was studied at the intra- andinterspecific levels within the genus Passiflora, with particular emphasis on the subgenusTacsonia and its relationship with the subgenusPassiflora. AFLP fingerprints were generated for35 accessions from 21 species, 14 of which belong to subgenusTacsonia, and four to subgenusPassiflora. The morphologically intermediateP. manicata (subgenusManicata) was also included, as wereP. trinervia (subgenusPsylanthus) and P.alnifolia (subgenus Decaloba). The two latter were used as outgroup species. Twoprimer combinations generated 34–80 scorable fragments pergenotype for a total of 260 fragments. Polymorphism analyses spannedthe levels from individual genotype to section. SubgenusTacsonia presented wide genetic variability atboth intra- and interspecific levels, particularly within wildspecies. The classification based on these markers generally agreeswell with current taxonomic definitions of common species, but lesswith definitions of sections. A geographical component was evident inthe intraspecific variation of P.mixta, P.tripartita var. mollissimaand P. tarminiana, withwider variation in Ecuador and Peru than in Colombia and Venezuela.These same three species, plus P.cumbalensis, formed adjacent clusters, showingcloser affinity with each other than with the other species ofsubgenus Tacsonia. As expected, P. manicata clustered at anintermediate position between subgenera Tacsoniaand Passiflora. 2002 2014-09-24T08:41:44Z 2014-09-24T08:41:44Z Journal Article https://hdl.handle.net/10568/43172 en Limited Access Springer
spellingShingle tropical fruits
passiflora
genetic markers
tacsonia
genetic variation
botany
taxonomy
frutas tropicales
marcadores genéticos
variación genética
botánica
taxonomía
Segura, Sergio D.
Coppens d'Eeckenbrugge, Geo
Bohórquez Cháux, Adriana
Ollitrault, P
Tohme, Joseph M.
An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia
title An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia
title_full An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia
title_fullStr An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia
title_full_unstemmed An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia
title_short An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia
title_sort aflp diversity study of the genus passiflora focusing on subgenus tacsonia
topic tropical fruits
passiflora
genetic markers
tacsonia
genetic variation
botany
taxonomy
frutas tropicales
marcadores genéticos
variación genética
botánica
taxonomía
url https://hdl.handle.net/10568/43172
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