Effect of cryopreservation on growth of different elite embryogenic cell lines of norway spruce (Picea Abies (L.) Karst.)
Callus from seven different elite embryogenic cell lines of Norway spruce originated from the controlled crossing conducted by Skogforsk (Forest Research Institute of Sweden) have been cryopreserved in liquid nitrogen using the slow freezing method with sorbitol (0.2 and 0.4 M) and DMSO (5% v/v) as...
| Main Authors: | , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2011
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/43075 |
| _version_ | 1855533894479118336 |
|---|---|
| author | Dedicova, Beata Nilsson, O Egertsdotter, U |
| author_browse | Dedicova, Beata Egertsdotter, U Nilsson, O |
| author_facet | Dedicova, Beata Nilsson, O Egertsdotter, U |
| author_sort | Dedicova, Beata |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Callus from seven different elite embryogenic cell lines of Norway spruce originated from the controlled crossing conducted by Skogforsk (Forest Research Institute of Sweden) have been cryopreserved in liquid nitrogen using the slow freezing method with sorbitol (0.2 and 0.4 M) and DMSO (5% v/v) as cryoprotectants. Cooloing of samples was performed in a programmable freezer Cryo Med 7452. The cryovials were inserted at +4°C and frozen at -0.3°C/min to -16°C. After 15 min at -16°C, to avoid rapid cooling and crystal formation in the cells, the cryovials were further cooled to -35°C at -0.3°C/min. At the end of the freezing program the cryovials were transferred and stored in a CBS V1500 liquid nitrogen storage unit at -196°C. After thawing and plating on proliferation medium, the recovered cells showed a short lag phase, after which they continued to grow. Lags in growth were also observed after the transfer to pre-maturation and maturation media. Somatic embryo maturation and plantlet regeneration occurred in all the tested embryogenic lines. |
| format | Journal Article |
| id | CGSpace43075 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2011 |
| publishDateRange | 2011 |
| publishDateSort | 2011 |
| record_format | dspace |
| spelling | CGSpace430752023-02-15T05:18:53Z Effect of cryopreservation on growth of different elite embryogenic cell lines of norway spruce (Picea Abies (L.) Karst.) Dedicova, Beata Nilsson, O Egertsdotter, U cryopreservation somatic embryogenesis picea abies criopreservación embriogénesis somática Callus from seven different elite embryogenic cell lines of Norway spruce originated from the controlled crossing conducted by Skogforsk (Forest Research Institute of Sweden) have been cryopreserved in liquid nitrogen using the slow freezing method with sorbitol (0.2 and 0.4 M) and DMSO (5% v/v) as cryoprotectants. Cooloing of samples was performed in a programmable freezer Cryo Med 7452. The cryovials were inserted at +4°C and frozen at -0.3°C/min to -16°C. After 15 min at -16°C, to avoid rapid cooling and crystal formation in the cells, the cryovials were further cooled to -35°C at -0.3°C/min. At the end of the freezing program the cryovials were transferred and stored in a CBS V1500 liquid nitrogen storage unit at -196°C. After thawing and plating on proliferation medium, the recovered cells showed a short lag phase, after which they continued to grow. Lags in growth were also observed after the transfer to pre-maturation and maturation media. Somatic embryo maturation and plantlet regeneration occurred in all the tested embryogenic lines. 2011 2014-09-24T08:41:35Z 2014-09-24T08:41:35Z Journal Article https://hdl.handle.net/10568/43075 en Limited Access Dedicova, B., Nilsson, O. and Egertsdotter, U. 2011. effect of cryopreservation on growth of different elite embryogenic cell lines of Norway spruce (Picea abies (l.) karst.). Acta Hort. (ISHS) 908:203-206 http://www.actahort.org/books/908/908_24.htm |
| spellingShingle | cryopreservation somatic embryogenesis picea abies criopreservación embriogénesis somática Dedicova, Beata Nilsson, O Egertsdotter, U Effect of cryopreservation on growth of different elite embryogenic cell lines of norway spruce (Picea Abies (L.) Karst.) |
| title | Effect of cryopreservation on growth of different elite embryogenic cell lines of norway spruce (Picea Abies (L.) Karst.) |
| title_full | Effect of cryopreservation on growth of different elite embryogenic cell lines of norway spruce (Picea Abies (L.) Karst.) |
| title_fullStr | Effect of cryopreservation on growth of different elite embryogenic cell lines of norway spruce (Picea Abies (L.) Karst.) |
| title_full_unstemmed | Effect of cryopreservation on growth of different elite embryogenic cell lines of norway spruce (Picea Abies (L.) Karst.) |
| title_short | Effect of cryopreservation on growth of different elite embryogenic cell lines of norway spruce (Picea Abies (L.) Karst.) |
| title_sort | effect of cryopreservation on growth of different elite embryogenic cell lines of norway spruce picea abies l karst |
| topic | cryopreservation somatic embryogenesis picea abies criopreservación embriogénesis somática |
| url | https://hdl.handle.net/10568/43075 |
| work_keys_str_mv | AT dedicovabeata effectofcryopreservationongrowthofdifferenteliteembryogeniccelllinesofnorwaysprucepiceaabieslkarst AT nilssono effectofcryopreservationongrowthofdifferenteliteembryogeniccelllinesofnorwaysprucepiceaabieslkarst AT egertsdotteru effectofcryopreservationongrowthofdifferenteliteembryogeniccelllinesofnorwaysprucepiceaabieslkarst |