Characterization of a phytoplasma associated with frogskin disease in cassava
Cassava frogskin disease (CFSD) is an economically important root disease of cassava (Manihot esculenta) in Colombia and other South American countries, including Brazil, Venezuela, Peru, Costa Rica, and Panama. The roots of severely affected plants are thin, making them unsuitable for consumptio...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Scientific Societies
2009
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/43053 |
| _version_ | 1855515782517096448 |
|---|---|
| author | Álvarez, E. Mejía de los Ríos, J.F. Llano Rodríguez, Germán Alberto Loke, John Bernard Calari, A Duduk, B Bertaccini, A |
| author_browse | Bertaccini, A Calari, A Duduk, B Llano Rodríguez, Germán Alberto Loke, John Bernard Mejía de los Ríos, J.F. Álvarez, E. |
| author_facet | Álvarez, E. Mejía de los Ríos, J.F. Llano Rodríguez, Germán Alberto Loke, John Bernard Calari, A Duduk, B Bertaccini, A |
| author_sort | Álvarez, E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava frogskin disease (CFSD) is an economically important root disease of cassava (Manihot
esculenta) in Colombia and other South American countries, including Brazil, Venezuela, Peru,
Costa Rica, and Panama. The roots of severely affected plants are thin, making them unsuitable
for consumption. In Colombia, phytoplasma infections were confirmed in 35 of 39 genotypes
exhibiting mild or severe CFSD symptoms either by direct or nested polymerase chain reaction
(PCR) assays employing ribosomal (r)RNA operon primer pairs. The CFSD-associated phytoplasmas
were identified as group 16SrIII strains by restriction fragment length polymorphism
(RFLP) and sequence analyses of amplified rDNA products, and results were corroborated by
PCRs employing group 16SrIII-specific rRNA gene or ribosomal protein (rp) gene primers.
Collectively, RFLP analyses indicated that CFSD strains differed from all phytoplasmas described
previously in group 16SrIII and, on this basis, the strains were tentatively assigned to
new ribosomal and ribosomal protein subgroups 16SrIII-L and rpIII-H, respectively. This is the
first molecular identification of a phytoplasma associated with CFSD in cassava in Colombia. |
| format | Journal Article |
| id | CGSpace43053 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace430532024-08-27T10:34:54Z Characterization of a phytoplasma associated with frogskin disease in cassava Álvarez, E. Mejía de los Ríos, J.F. Llano Rodríguez, Germán Alberto Loke, John Bernard Calari, A Duduk, B Bertaccini, A manihot esculenta frogskin disease roots phytoplasmas pcr ametryn genotypes tissue analysis enfermedad cuero de sapo raíces fitoplasmas genotipos análisis de tejidos Cassava frogskin disease (CFSD) is an economically important root disease of cassava (Manihot esculenta) in Colombia and other South American countries, including Brazil, Venezuela, Peru, Costa Rica, and Panama. The roots of severely affected plants are thin, making them unsuitable for consumption. In Colombia, phytoplasma infections were confirmed in 35 of 39 genotypes exhibiting mild or severe CFSD symptoms either by direct or nested polymerase chain reaction (PCR) assays employing ribosomal (r)RNA operon primer pairs. The CFSD-associated phytoplasmas were identified as group 16SrIII strains by restriction fragment length polymorphism (RFLP) and sequence analyses of amplified rDNA products, and results were corroborated by PCRs employing group 16SrIII-specific rRNA gene or ribosomal protein (rp) gene primers. Collectively, RFLP analyses indicated that CFSD strains differed from all phytoplasmas described previously in group 16SrIII and, on this basis, the strains were tentatively assigned to new ribosomal and ribosomal protein subgroups 16SrIII-L and rpIII-H, respectively. This is the first molecular identification of a phytoplasma associated with CFSD in cassava in Colombia. 2009-11 2014-09-24T08:41:33Z 2014-09-24T08:41:33Z Journal Article https://hdl.handle.net/10568/43053 en Open Access Scientific Societies |
| spellingShingle | manihot esculenta frogskin disease roots phytoplasmas pcr ametryn genotypes tissue analysis enfermedad cuero de sapo raíces fitoplasmas genotipos análisis de tejidos Álvarez, E. Mejía de los Ríos, J.F. Llano Rodríguez, Germán Alberto Loke, John Bernard Calari, A Duduk, B Bertaccini, A Characterization of a phytoplasma associated with frogskin disease in cassava |
| title | Characterization of a phytoplasma associated with frogskin disease in cassava |
| title_full | Characterization of a phytoplasma associated with frogskin disease in cassava |
| title_fullStr | Characterization of a phytoplasma associated with frogskin disease in cassava |
| title_full_unstemmed | Characterization of a phytoplasma associated with frogskin disease in cassava |
| title_short | Characterization of a phytoplasma associated with frogskin disease in cassava |
| title_sort | characterization of a phytoplasma associated with frogskin disease in cassava |
| topic | manihot esculenta frogskin disease roots phytoplasmas pcr ametryn genotypes tissue analysis enfermedad cuero de sapo raíces fitoplasmas genotipos análisis de tejidos |
| url | https://hdl.handle.net/10568/43053 |
| work_keys_str_mv | AT alvareze characterizationofaphytoplasmaassociatedwithfrogskindiseaseincassava AT mejiadelosriosjf characterizationofaphytoplasmaassociatedwithfrogskindiseaseincassava AT llanorodriguezgermanalberto characterizationofaphytoplasmaassociatedwithfrogskindiseaseincassava AT lokejohnbernard characterizationofaphytoplasmaassociatedwithfrogskindiseaseincassava AT calaria characterizationofaphytoplasmaassociatedwithfrogskindiseaseincassava AT dudukb characterizationofaphytoplasmaassociatedwithfrogskindiseaseincassava AT bertaccinia characterizationofaphytoplasmaassociatedwithfrogskindiseaseincassava |