Identifying resistance gene analogs associated with resistances to different pathogens in common beans
A polymerase chain reaction approach using degenerate primers that targeted the conserved domains of cloned plant disease resistance genes (R genes) was used to isolate a set of 15 resistance gene analogs (RGAs) from common bean (Phaseolus vulgaris). Eight different classes of RGAs were obtained fro...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Scientific Societies
2003
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/42953 |
| _version_ | 1855539150800814080 |
|---|---|
| author | López, C.E. Acosta, I.F. Jara, Carlos E. Pedraza García, Fabio Gaitán Solís, Eliana Gallego Sánchez, Gerardo J. Beebe, Stephen E. Tohme, Joseph M. |
| author_browse | Acosta, I.F. Beebe, Stephen E. Gaitán Solís, Eliana Gallego Sánchez, Gerardo J. Jara, Carlos E. López, C.E. Pedraza García, Fabio Tohme, Joseph M. |
| author_facet | López, C.E. Acosta, I.F. Jara, Carlos E. Pedraza García, Fabio Gaitán Solís, Eliana Gallego Sánchez, Gerardo J. Beebe, Stephen E. Tohme, Joseph M. |
| author_sort | López, C.E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A polymerase chain reaction approach using degenerate primers that targeted the conserved domains of cloned plant disease resistance genes (R genes) was used to isolate a set of 15 resistance gene analogs (RGAs) from common bean (Phaseolus vulgaris). Eight different classes of RGAs were obtained from nucleotide binding site (NBS)-based primers and seven from not previously described Toll/Interleukin-1 receptor-like (TIR)-based primers. Putative amino acid sequences of RGAs were significantly similar to R genes and contained additional conserved motifs. The NBS-type RGAs were classified in two subgroups according to the expected final residue in the kinase-2 motif. Eleven RGAs were mapped at 19 loci on eight linkage groups of the common bean genetic map constructed at Centro Internacional de Agricultura Tropical. Genetic linkage was shown for eight RGAs with partial resistance to anthracnose, angular leaf spot (ALS) and Bean golden yellow mosaic virus (BGYMV). RGA1 and RGA2 were associated with resistance loci to anthracnose and BGYMV and were part of two clusters of R genes previously described. A new major cluster was detected by RGA7 and explained up to 63.9% of resistance to ALS and has a putative contribution to anthracnose resistance. These results show the usefulness of RGAs as candidate genes to detect and eventually isolate numerous R genes in common bean. |
| format | Journal Article |
| id | CGSpace42953 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2003 |
| publishDateRange | 2003 |
| publishDateSort | 2003 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace429532024-04-25T06:01:44Z Identifying resistance gene analogs associated with resistances to different pathogens in common beans López, C.E. Acosta, I.F. Jara, Carlos E. Pedraza García, Fabio Gaitán Solís, Eliana Gallego Sánchez, Gerardo J. Beebe, Stephen E. Tohme, Joseph M. phaseolus vulgaris genes disease resistance genetic resistance genetic markers resistencia a la enfermedad resistencia genética marcadores genéticos A polymerase chain reaction approach using degenerate primers that targeted the conserved domains of cloned plant disease resistance genes (R genes) was used to isolate a set of 15 resistance gene analogs (RGAs) from common bean (Phaseolus vulgaris). Eight different classes of RGAs were obtained from nucleotide binding site (NBS)-based primers and seven from not previously described Toll/Interleukin-1 receptor-like (TIR)-based primers. Putative amino acid sequences of RGAs were significantly similar to R genes and contained additional conserved motifs. The NBS-type RGAs were classified in two subgroups according to the expected final residue in the kinase-2 motif. Eleven RGAs were mapped at 19 loci on eight linkage groups of the common bean genetic map constructed at Centro Internacional de Agricultura Tropical. Genetic linkage was shown for eight RGAs with partial resistance to anthracnose, angular leaf spot (ALS) and Bean golden yellow mosaic virus (BGYMV). RGA1 and RGA2 were associated with resistance loci to anthracnose and BGYMV and were part of two clusters of R genes previously described. A new major cluster was detected by RGA7 and explained up to 63.9% of resistance to ALS and has a putative contribution to anthracnose resistance. These results show the usefulness of RGAs as candidate genes to detect and eventually isolate numerous R genes in common bean. 2003-01 2014-09-24T07:58:49Z 2014-09-24T07:58:49Z Journal Article https://hdl.handle.net/10568/42953 en Open Access Scientific Societies López, Camilo Ernesto; Acosta, Iván F.; Jara, Carlos E.; Pedraza García, Fabio; Gaitán Solís, Eliana; Gallego, Gerardo J.; Beebe, Stephen E.; Tohme M., Joseph. 2003. Identifying resistance gene analogs associated with resistances to different pathogens in common beans . Phytopathology (USA) 93(1):88-95. |
| spellingShingle | phaseolus vulgaris genes disease resistance genetic resistance genetic markers resistencia a la enfermedad resistencia genética marcadores genéticos López, C.E. Acosta, I.F. Jara, Carlos E. Pedraza García, Fabio Gaitán Solís, Eliana Gallego Sánchez, Gerardo J. Beebe, Stephen E. Tohme, Joseph M. Identifying resistance gene analogs associated with resistances to different pathogens in common beans |
| title | Identifying resistance gene analogs associated with resistances to different pathogens in common beans |
| title_full | Identifying resistance gene analogs associated with resistances to different pathogens in common beans |
| title_fullStr | Identifying resistance gene analogs associated with resistances to different pathogens in common beans |
| title_full_unstemmed | Identifying resistance gene analogs associated with resistances to different pathogens in common beans |
| title_short | Identifying resistance gene analogs associated with resistances to different pathogens in common beans |
| title_sort | identifying resistance gene analogs associated with resistances to different pathogens in common beans |
| topic | phaseolus vulgaris genes disease resistance genetic resistance genetic markers resistencia a la enfermedad resistencia genética marcadores genéticos |
| url | https://hdl.handle.net/10568/42953 |
| work_keys_str_mv | AT lopezce identifyingresistancegeneanalogsassociatedwithresistancestodifferentpathogensincommonbeans AT acostaif identifyingresistancegeneanalogsassociatedwithresistancestodifferentpathogensincommonbeans AT jaracarlose identifyingresistancegeneanalogsassociatedwithresistancestodifferentpathogensincommonbeans AT pedrazagarciafabio identifyingresistancegeneanalogsassociatedwithresistancestodifferentpathogensincommonbeans AT gaitansoliseliana identifyingresistancegeneanalogsassociatedwithresistancestodifferentpathogensincommonbeans AT gallegosanchezgerardoj identifyingresistancegeneanalogsassociatedwithresistancestodifferentpathogensincommonbeans AT beebestephene identifyingresistancegeneanalogsassociatedwithresistancestodifferentpathogensincommonbeans AT tohmejosephm identifyingresistancegeneanalogsassociatedwithresistancestodifferentpathogensincommonbeans |