Identification of microsatellite markers linked to the blast resistance gene Pi-1(t) in rice
The present work was conducted to identify microsatellite markers linked to the rice blast resistance gene Pi-1(t) for a marker-assisted selection program. Twenty-four primer pairs corresponding to 19 microsatellite loci were selected from the Gramene database (www. gramene.org) considering their re...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2008
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/42949 |
| _version_ | 1855522526957928448 |
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| author | Fuentes, J.L Correa Victoria, Fernando J. Escobar, F. Prado Patiño, Gustavo A. Aricapa, María Girlena Duque E., Myriam Cristina Tohme, Joseph M. |
| author_browse | Aricapa, María Girlena Correa Victoria, Fernando J. Duque E., Myriam Cristina Escobar, F. Fuentes, J.L Prado Patiño, Gustavo A. Tohme, Joseph M. |
| author_facet | Fuentes, J.L Correa Victoria, Fernando J. Escobar, F. Prado Patiño, Gustavo A. Aricapa, María Girlena Duque E., Myriam Cristina Tohme, Joseph M. |
| author_sort | Fuentes, J.L |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The present work was conducted to identify microsatellite markers linked to the rice blast resistance gene Pi-1(t) for a marker-assisted selection program. Twenty-four primer pairs corresponding to 19 microsatellite loci were selected from the Gramene database (www. gramene.org) considering their relative proximity to Pi-1(t) gene in the current rice genetic map. Progenitors and DNA bulks of resistant and susceptible families from F3 segregating populations of a cross between the near-isogenic lines C101LAC (resistant) and C101A51 (susceptible) were used to identify polymorphic microsatellite markers associated to this gene through bulked segregant analysis. Putative molecular markers linked to the blast resistance gene Pi-1(t) were then used on the whole progeny for linkage analysis. Additionally, the diagnostic potential of the microsatellite markers associated to the resistance gene was also evaluated on 17 rice varieties planted in Latin America by amplification of the specific resistant alleles for the gene in each genotype. Comparing with greenhouse phenotypic evaluations for blast resistance, the usefulness of the highly linked microsatellite markers to identify resistant rice genotypes was evaluated. As expected, the phenotypic segregation in the F3 generation agreed to the expected segregation ratio for a single gene model. Of the 24 microsatellite sequences tested, six resulted polymorphic and linked to the gene. Two markers (RM1233*I and RM224) mapped in the same position (0.0 cM) with the Pi-1(t) gene. Other three markers corresponding to the same genetic locus were located at 18.5 cM above the resistance gene, while another marker was positioned at 23.8 cM below the gene. Microsatellite analysis on elite rice varieties with different genetic background showed that all known sources of blast resistance included in this study carry the specific Pi-1(t) allele. Results are discussed considering the potential utility of the microsatellite markers found, for MAS in rice breeding programs aiming at developing rice varieties with durable blast resistance based on a combination of resistance genes. |
| format | Journal Article |
| id | CGSpace42949 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2008 |
| publishDateRange | 2008 |
| publishDateSort | 2008 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace429492024-08-27T10:35:40Z Identification of microsatellite markers linked to the blast resistance gene Pi-1(t) in rice Fuentes, J.L Correa Victoria, Fernando J. Escobar, F. Prado Patiño, Gustavo A. Aricapa, María Girlena Duque E., Myriam Cristina Tohme, Joseph M. oryza sativa rice genetic resistance genes pyricularia grisea magnaporthe grisea microsatellites arroz resistencia genética microsatélites The present work was conducted to identify microsatellite markers linked to the rice blast resistance gene Pi-1(t) for a marker-assisted selection program. Twenty-four primer pairs corresponding to 19 microsatellite loci were selected from the Gramene database (www. gramene.org) considering their relative proximity to Pi-1(t) gene in the current rice genetic map. Progenitors and DNA bulks of resistant and susceptible families from F3 segregating populations of a cross between the near-isogenic lines C101LAC (resistant) and C101A51 (susceptible) were used to identify polymorphic microsatellite markers associated to this gene through bulked segregant analysis. Putative molecular markers linked to the blast resistance gene Pi-1(t) were then used on the whole progeny for linkage analysis. Additionally, the diagnostic potential of the microsatellite markers associated to the resistance gene was also evaluated on 17 rice varieties planted in Latin America by amplification of the specific resistant alleles for the gene in each genotype. Comparing with greenhouse phenotypic evaluations for blast resistance, the usefulness of the highly linked microsatellite markers to identify resistant rice genotypes was evaluated. As expected, the phenotypic segregation in the F3 generation agreed to the expected segregation ratio for a single gene model. Of the 24 microsatellite sequences tested, six resulted polymorphic and linked to the gene. Two markers (RM1233*I and RM224) mapped in the same position (0.0 cM) with the Pi-1(t) gene. Other three markers corresponding to the same genetic locus were located at 18.5 cM above the resistance gene, while another marker was positioned at 23.8 cM below the gene. Microsatellite analysis on elite rice varieties with different genetic background showed that all known sources of blast resistance included in this study carry the specific Pi-1(t) allele. Results are discussed considering the potential utility of the microsatellite markers found, for MAS in rice breeding programs aiming at developing rice varieties with durable blast resistance based on a combination of resistance genes. 2008-04 2014-09-24T07:58:49Z 2014-09-24T07:58:49Z Journal Article https://hdl.handle.net/10568/42949 en Limited Access Springer |
| spellingShingle | oryza sativa rice genetic resistance genes pyricularia grisea magnaporthe grisea microsatellites arroz resistencia genética microsatélites Fuentes, J.L Correa Victoria, Fernando J. Escobar, F. Prado Patiño, Gustavo A. Aricapa, María Girlena Duque E., Myriam Cristina Tohme, Joseph M. Identification of microsatellite markers linked to the blast resistance gene Pi-1(t) in rice |
| title | Identification of microsatellite markers linked to the blast resistance gene Pi-1(t) in rice |
| title_full | Identification of microsatellite markers linked to the blast resistance gene Pi-1(t) in rice |
| title_fullStr | Identification of microsatellite markers linked to the blast resistance gene Pi-1(t) in rice |
| title_full_unstemmed | Identification of microsatellite markers linked to the blast resistance gene Pi-1(t) in rice |
| title_short | Identification of microsatellite markers linked to the blast resistance gene Pi-1(t) in rice |
| title_sort | identification of microsatellite markers linked to the blast resistance gene pi 1 t in rice |
| topic | oryza sativa rice genetic resistance genes pyricularia grisea magnaporthe grisea microsatellites arroz resistencia genética microsatélites |
| url | https://hdl.handle.net/10568/42949 |
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