Genetic transformation of the tropical forage legume Stylosanthes guianensis with a rice-chitinase gene confers resistance to Rhizoctonia foliar blight disease
Stylosanthes guianensis is a diverse tropical and subtropical forage legume of great potential. Foliar blight disease, caused by Rhizoctonia solani AG-1, can be a significant constraint to the legume’s production in some areas. The pathogen has a broad range of host plant species, and can survive as...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2005
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| Online Access: | https://hdl.handle.net/10568/42904 |
| _version_ | 1855542801363632128 |
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| author | Kelemu, Segenet Changshun, J Guixi, H Segura, G. |
| author_browse | Changshun, J Guixi, H Kelemu, Segenet Segura, G. |
| author_facet | Kelemu, Segenet Changshun, J Guixi, H Segura, G. |
| author_sort | Kelemu, Segenet |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Stylosanthes guianensis is a diverse tropical and subtropical forage legume of great potential. Foliar blight disease, caused by Rhizoctonia solani AG-1, can be a significant constraint to the legume’s production in some areas. The pathogen has a broad range of host plant species, and can survive as sclerotia for long periods in soil or plant debris. No sources of resistance in Stylosanthes are known. Cost-effective disease management strategies are, therefore, needed for this host-pathogen system. Chitinases, which catalyze the hydrolysis of the b-1,4 linkages of the N-acetyl-D-glucosamine polymer chitin of fungal cell walls, are expressed in plants in response to infections by pathogens and some abiotic stresses. In this study, a basic chitinase-encoding gene, isolated from rice, was introduced into the widely distributed S. guianensis accession CIAT 184, using Agrobacterium tumefaciens. A 1.1-kb rice genomic DNA fragment containing the chitinase gene was cloned into a transformation vector, pCAMBIA2301. The vector carried the CaMV 35S promoter, the neomycin phosphotransferase (nptII) gene and the gusA reporter gene. The presence of the chitinase gene in transgenic Stylosanthes plants was determined by dot blot analysis. Transgenic plants expressed higher levels of resistance to R. solani than did control plants. Progenies of a selfed transgenic plant showed segregation for resistance at a ratio of 3:1. |
| format | Journal Article |
| id | CGSpace42904 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2005 |
| publishDateRange | 2005 |
| publishDateSort | 2005 |
| record_format | dspace |
| spelling | CGSpace429042023-06-13T06:31:37Z Genetic transformation of the tropical forage legume Stylosanthes guianensis with a rice-chitinase gene confers resistance to Rhizoctonia foliar blight disease Kelemu, Segenet Changshun, J Guixi, H Segura, G. feed crops stylosanthes guianensis rhizoctonia solani genetic transformation transgenic plants chitinase disease resistance genetic resistance transformación genética plantas transgénicas quitinasa resistencia a la enfermedad resistencia genética Stylosanthes guianensis is a diverse tropical and subtropical forage legume of great potential. Foliar blight disease, caused by Rhizoctonia solani AG-1, can be a significant constraint to the legume’s production in some areas. The pathogen has a broad range of host plant species, and can survive as sclerotia for long periods in soil or plant debris. No sources of resistance in Stylosanthes are known. Cost-effective disease management strategies are, therefore, needed for this host-pathogen system. Chitinases, which catalyze the hydrolysis of the b-1,4 linkages of the N-acetyl-D-glucosamine polymer chitin of fungal cell walls, are expressed in plants in response to infections by pathogens and some abiotic stresses. In this study, a basic chitinase-encoding gene, isolated from rice, was introduced into the widely distributed S. guianensis accession CIAT 184, using Agrobacterium tumefaciens. A 1.1-kb rice genomic DNA fragment containing the chitinase gene was cloned into a transformation vector, pCAMBIA2301. The vector carried the CaMV 35S promoter, the neomycin phosphotransferase (nptII) gene and the gusA reporter gene. The presence of the chitinase gene in transgenic Stylosanthes plants was determined by dot blot analysis. Transgenic plants expressed higher levels of resistance to R. solani than did control plants. Progenies of a selfed transgenic plant showed segregation for resistance at a ratio of 3:1. 2005-10 2014-09-24T07:58:45Z 2014-09-24T07:58:45Z Journal Article https://hdl.handle.net/10568/42904 en Open Access Kelemu, S.; Changshun, J.; Guixi, H.;Segura, G. 2005. Genetic transformation of the tropical forage legume Stylosanthes guianensis with a rice-chitinase gene confers resistance to Rhizoctonia foliar blight disease. African Journal of Biotechnology 4(10), p. 1025-1033. |
| spellingShingle | feed crops stylosanthes guianensis rhizoctonia solani genetic transformation transgenic plants chitinase disease resistance genetic resistance transformación genética plantas transgénicas quitinasa resistencia a la enfermedad resistencia genética Kelemu, Segenet Changshun, J Guixi, H Segura, G. Genetic transformation of the tropical forage legume Stylosanthes guianensis with a rice-chitinase gene confers resistance to Rhizoctonia foliar blight disease |
| title | Genetic transformation of the tropical forage legume Stylosanthes guianensis with a rice-chitinase gene confers resistance to Rhizoctonia foliar blight disease |
| title_full | Genetic transformation of the tropical forage legume Stylosanthes guianensis with a rice-chitinase gene confers resistance to Rhizoctonia foliar blight disease |
| title_fullStr | Genetic transformation of the tropical forage legume Stylosanthes guianensis with a rice-chitinase gene confers resistance to Rhizoctonia foliar blight disease |
| title_full_unstemmed | Genetic transformation of the tropical forage legume Stylosanthes guianensis with a rice-chitinase gene confers resistance to Rhizoctonia foliar blight disease |
| title_short | Genetic transformation of the tropical forage legume Stylosanthes guianensis with a rice-chitinase gene confers resistance to Rhizoctonia foliar blight disease |
| title_sort | genetic transformation of the tropical forage legume stylosanthes guianensis with a rice chitinase gene confers resistance to rhizoctonia foliar blight disease |
| topic | feed crops stylosanthes guianensis rhizoctonia solani genetic transformation transgenic plants chitinase disease resistance genetic resistance transformación genética plantas transgénicas quitinasa resistencia a la enfermedad resistencia genética |
| url | https://hdl.handle.net/10568/42904 |
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