Genetic structure and population dynamics of Xanthomonas axonopodis pv. manihotis in Colombia from 1995 to 1999

Restriction fragment length polymorphisms (RFLPs) were used to study the population genetics and temporal dynamics of the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis. The population dynamics were addressed by comparing samples collected from 1995 to 1999 from six locations, spann...

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Main Authors: Restrepo, S., Velez, C.M., Duque E., Myriam Cristina, Verdier, Valerie M.
Format: Journal Article
Language:Inglés
Published: American Society for Microbiology 2004
Subjects:
Online Access:https://hdl.handle.net/10568/42903
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author Restrepo, S.
Velez, C.M.
Duque E., Myriam Cristina
Verdier, Valerie M.
author_browse Duque E., Myriam Cristina
Restrepo, S.
Velez, C.M.
Verdier, Valerie M.
author_facet Restrepo, S.
Velez, C.M.
Duque E., Myriam Cristina
Verdier, Valerie M.
author_sort Restrepo, S.
collection Repository of Agricultural Research Outputs (CGSpace)
description Restriction fragment length polymorphisms (RFLPs) were used to study the population genetics and temporal dynamics of the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis. The population dynamics were addressed by comparing samples collected from 1995 to 1999 from six locations, spanning four different edaphoclimatic zones (ECZs). Forty-five different X. axonopodis pv. manihotis RFLP types or haplotypes were identified between 1995 and 1999. High genetic diversity of the X. axonopodis pv. manihotis strains was evident within most of the fields sampled. In all but one site, diversity decreased over time within fields. Haplotype frequencies significantly differed over the years in all but one location. Studies of the rate of change of X. axonopodis pv. manihotis populations during the cropping cycle in two sites showed significant changes in the haplotype frequencies but not composition. However, variations in pathotype composition were observed from one year to the next at a single site in ECZs 1 and 2 and new pathotypes were described after 1997 in these ECZs, thus revealing the dramatic change in the pathogen population structure of X. axonopodis pv. manihotis. Disease incidence was used to show the progress of cassava bacterial blight in Colombia during the 5-year period in different ecosystems. Low disease incidence values were correlated with low rainfall in 1997 in ECZ 1.
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spelling CGSpace429032024-08-27T10:35:00Z Genetic structure and population dynamics of Xanthomonas axonopodis pv. manihotis in Colombia from 1995 to 1999 Restrepo, S. Velez, C.M. Duque E., Myriam Cristina Verdier, Valerie M. manihot esculenta xanthomonas axonopodis population genetics rflp genetic variation genética de poblaciones variación genética ecology food science biotechnology Restriction fragment length polymorphisms (RFLPs) were used to study the population genetics and temporal dynamics of the cassava bacterial pathogen Xanthomonas axonopodis pv. manihotis. The population dynamics were addressed by comparing samples collected from 1995 to 1999 from six locations, spanning four different edaphoclimatic zones (ECZs). Forty-five different X. axonopodis pv. manihotis RFLP types or haplotypes were identified between 1995 and 1999. High genetic diversity of the X. axonopodis pv. manihotis strains was evident within most of the fields sampled. In all but one site, diversity decreased over time within fields. Haplotype frequencies significantly differed over the years in all but one location. Studies of the rate of change of X. axonopodis pv. manihotis populations during the cropping cycle in two sites showed significant changes in the haplotype frequencies but not composition. However, variations in pathotype composition were observed from one year to the next at a single site in ECZs 1 and 2 and new pathotypes were described after 1997 in these ECZs, thus revealing the dramatic change in the pathogen population structure of X. axonopodis pv. manihotis. Disease incidence was used to show the progress of cassava bacterial blight in Colombia during the 5-year period in different ecosystems. Low disease incidence values were correlated with low rainfall in 1997 in ECZ 1. 2004-01 2014-09-24T07:58:44Z 2014-09-24T07:58:44Z Journal Article https://hdl.handle.net/10568/42903 en Open Access American Society for Microbiology
spellingShingle manihot esculenta
xanthomonas axonopodis
population genetics
rflp
genetic variation
genética de poblaciones
variación genética
ecology
food science
biotechnology
Restrepo, S.
Velez, C.M.
Duque E., Myriam Cristina
Verdier, Valerie M.
Genetic structure and population dynamics of Xanthomonas axonopodis pv. manihotis in Colombia from 1995 to 1999
title Genetic structure and population dynamics of Xanthomonas axonopodis pv. manihotis in Colombia from 1995 to 1999
title_full Genetic structure and population dynamics of Xanthomonas axonopodis pv. manihotis in Colombia from 1995 to 1999
title_fullStr Genetic structure and population dynamics of Xanthomonas axonopodis pv. manihotis in Colombia from 1995 to 1999
title_full_unstemmed Genetic structure and population dynamics of Xanthomonas axonopodis pv. manihotis in Colombia from 1995 to 1999
title_short Genetic structure and population dynamics of Xanthomonas axonopodis pv. manihotis in Colombia from 1995 to 1999
title_sort genetic structure and population dynamics of xanthomonas axonopodis pv manihotis in colombia from 1995 to 1999
topic manihot esculenta
xanthomonas axonopodis
population genetics
rflp
genetic variation
genética de poblaciones
variación genética
ecology
food science
biotechnology
url https://hdl.handle.net/10568/42903
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