Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz)
Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease of cassava (Manihot esculenta Crantz) in Africa and South America. Planting resistant varieties is the preferred method of disease control. Recent genetic mapping of an F1 cross (TMS 30572 × CM 2...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Springer
2000
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| Online Access: | https://hdl.handle.net/10568/42899 |
| _version_ | 1855538176146276352 |
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| author | Jorge, V. Fregene, Martin A. Duque E., Myriam Cristina Bonierbale, Merideth W. Tohme, Joseph M. Verdier, Valerie M. |
| author_browse | Bonierbale, Merideth W. Duque E., Myriam Cristina Fregene, Martin A. Jorge, V. Tohme, Joseph M. Verdier, Valerie M. |
| author_facet | Jorge, V. Fregene, Martin A. Duque E., Myriam Cristina Bonierbale, Merideth W. Tohme, Joseph M. Verdier, Valerie M. |
| author_sort | Jorge, V. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease of cassava (Manihot esculenta Crantz) in Africa and South America. Planting resistant varieties is the preferred method of disease control. Recent genetic mapping of an F1 cross (TMS 30572 × CM 2177–2) led to the construction of the first molecular genetic map of cassava. To better understand the genetics of resistance to CBB, we evaluated individuals of the F1 cross for CBB resistance by controlled greenhouse inoculations and visually assessed symptoms on days 7, 15, and 30 days after inoculation, using a scale where 0 = no disease and 5 = maximum susceptibility. Five Xam strains were used: CIO-84, CIO-1, CIO-136, CIO-295, and ORST X-27. Area under the disease progress curve (AUDPC) was used as a quantitative measure of resistance in QTL analysis by single-marker regression. Based on the AUDPC values, eight QTLs (quantitative trait loci), located on linkage groups B, D, L, N, and X of the female-derived framework map, were found to explain 9–20% of the phenotypic variance of the crop’s response to the five Xam strains. With the male-derived framework map, four QTLs on linkage groups G and C explained 10.7–27.1% of the variance. A scheme to confirm the usefulness of these markers in evaluating segregating populations for resistance to CBB is proposed. |
| format | Journal Article |
| id | CGSpace42899 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2000 |
| publishDateRange | 2000 |
| publishDateSort | 2000 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace428992024-08-27T10:37:02Z Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz) Jorge, V. Fregene, Martin A. Duque E., Myriam Cristina Bonierbale, Merideth W. Tohme, Joseph M. Verdier, Valerie M. xanthomonas axonopodis manihot esculenta disease resistance genetic maps resistencia a la enfermedad mapas genéticos Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease of cassava (Manihot esculenta Crantz) in Africa and South America. Planting resistant varieties is the preferred method of disease control. Recent genetic mapping of an F1 cross (TMS 30572 × CM 2177–2) led to the construction of the first molecular genetic map of cassava. To better understand the genetics of resistance to CBB, we evaluated individuals of the F1 cross for CBB resistance by controlled greenhouse inoculations and visually assessed symptoms on days 7, 15, and 30 days after inoculation, using a scale where 0 = no disease and 5 = maximum susceptibility. Five Xam strains were used: CIO-84, CIO-1, CIO-136, CIO-295, and ORST X-27. Area under the disease progress curve (AUDPC) was used as a quantitative measure of resistance in QTL analysis by single-marker regression. Based on the AUDPC values, eight QTLs (quantitative trait loci), located on linkage groups B, D, L, N, and X of the female-derived framework map, were found to explain 9–20% of the phenotypic variance of the crop’s response to the five Xam strains. With the male-derived framework map, four QTLs on linkage groups G and C explained 10.7–27.1% of the variance. A scheme to confirm the usefulness of these markers in evaluating segregating populations for resistance to CBB is proposed. 2000-10 2014-09-24T07:58:44Z 2014-09-24T07:58:44Z Journal Article https://hdl.handle.net/10568/42899 en Open Access Springer Jorge, Veronique; Fregene, Martin A.; Duque E., Myriam Cristina; Bonierbale, Merideth; Tohme, Joseph M. ; Verdier, Valerie (2001). Genetic Mapping of Resistance to bacterial blight disease on cassava (Manihot esculenta crantz). Theoretical and Applied Genetics 101:865-872 |
| spellingShingle | xanthomonas axonopodis manihot esculenta disease resistance genetic maps resistencia a la enfermedad mapas genéticos Jorge, V. Fregene, Martin A. Duque E., Myriam Cristina Bonierbale, Merideth W. Tohme, Joseph M. Verdier, Valerie M. Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz) |
| title | Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz) |
| title_full | Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz) |
| title_fullStr | Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz) |
| title_full_unstemmed | Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz) |
| title_short | Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz) |
| title_sort | genetic mapping of resistance to bacterial blight disease in cassava manihot esculenta crantz |
| topic | xanthomonas axonopodis manihot esculenta disease resistance genetic maps resistencia a la enfermedad mapas genéticos |
| url | https://hdl.handle.net/10568/42899 |
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