Evidence for biological nitrification inhibition in Brachiaria pastures
Nitrification, a key process in the global nitrogen cycle that generates nitrate through microbial activity, may enhance losses of fertilizer nitrogen by leaching and denitrification. Certain plants can suppress soil-nitrification by releasing inhibitors from roots, a phenomenon termed biological ni...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
National Academy of Sciences
2009
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/42836 |
| _version_ | 1855523040183451648 |
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| author | Subbaraoa, Guntur V. Nakahara, K. Hurtado, M.P. Ono, H Moreta Mejia, DE Salcedo, A.F. Yoshihashi, AT Ishikawa, T. Ishitani, Manabu Ohnishi, M Yoshida, M Rondón, Marco Antonio Rao, Idupulapati M. Lascano Aguilar, Carlos Eduardo Berry, W.L. Ito, O. |
| author_browse | Berry, W.L. Hurtado, M.P. Ishikawa, T. Ishitani, Manabu Ito, O. Lascano Aguilar, Carlos Eduardo Moreta Mejia, DE Nakahara, K. Ohnishi, M Ono, H Rao, Idupulapati M. Rondón, Marco Antonio Salcedo, A.F. Subbaraoa, Guntur V. Yoshida, M Yoshihashi, AT |
| author_facet | Subbaraoa, Guntur V. Nakahara, K. Hurtado, M.P. Ono, H Moreta Mejia, DE Salcedo, A.F. Yoshihashi, AT Ishikawa, T. Ishitani, Manabu Ohnishi, M Yoshida, M Rondón, Marco Antonio Rao, Idupulapati M. Lascano Aguilar, Carlos Eduardo Berry, W.L. Ito, O. |
| author_sort | Subbaraoa, Guntur V. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Nitrification, a key process in the global nitrogen cycle that generates nitrate through microbial activity, may enhance losses of fertilizer nitrogen by leaching and denitrification. Certain plants can suppress soil-nitrification by releasing inhibitors from roots, a phenomenon termed biological nitrification inhibition (BNI). Here, we report the discovery of an effective nitrification inhibitor in the root-exudates of the tropical forage grass Brachiaria humidicola (Rendle) Schweick. Named ‘‘brachialactone,’’ this inhibitor is a recently discovered cyclic diterpene with a unique 5-8-5-membered ring system and a -lactone ring. It contributed 60–90% of the inhibitory activity released from the roots of this tropical grass. Unlike nitrapyrin (a synthetic nitrification inhibitor), which affects only the ammonia monooxygenase (AMO) pathway, brachialactone appears to block both AMO and hydroxylamine oxidoreductase enzymatic pathways in Nitrosomonas. Release of this inhibitor is a regulated plant function, triggered and sustained by the availability of ammonium (NH4 ) in the root environment. Brachialactone release is restricted to those roots that are directly exposed to NH4 . Within 3 years of establishment, Brachiaria pastures have suppressed soil nitrifier populations (determined as amoA genes; ammonia-oxidizing bacteria and ammonia-oxidizing archaea), along with nitrification and nitrous oxide emissions. These findings provide direct evidence for the existence and active regulation of a nitrification inhibitor (or inhibitors) release from tropical pasture root systems. Exploiting the BNI function could become a powerful strategy toward the development of low-nitrifying agronomic systems, benefiting both agriculture and the environment. |
| format | Journal Article |
| id | CGSpace42836 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | National Academy of Sciences |
| publisherStr | National Academy of Sciences |
| record_format | dspace |
| spelling | CGSpace428362025-03-11T12:14:31Z Evidence for biological nitrification inhibition in Brachiaria pastures Subbaraoa, Guntur V. Nakahara, K. Hurtado, M.P. Ono, H Moreta Mejia, DE Salcedo, A.F. Yoshihashi, AT Ishikawa, T. Ishitani, Manabu Ohnishi, M Yoshida, M Rondón, Marco Antonio Rao, Idupulapati M. Lascano Aguilar, Carlos Eduardo Berry, W.L. Ito, O. feed crops brachiaria humidicola climate change nitrous oxide nitrification nitrification inhibitors nitrogen cycle pastures cambio climático oxido nitroso nitrificación inhibidores de la nitrificacion ciclo del nitrógeno pastizales Nitrification, a key process in the global nitrogen cycle that generates nitrate through microbial activity, may enhance losses of fertilizer nitrogen by leaching and denitrification. Certain plants can suppress soil-nitrification by releasing inhibitors from roots, a phenomenon termed biological nitrification inhibition (BNI). Here, we report the discovery of an effective nitrification inhibitor in the root-exudates of the tropical forage grass Brachiaria humidicola (Rendle) Schweick. Named ‘‘brachialactone,’’ this inhibitor is a recently discovered cyclic diterpene with a unique 5-8-5-membered ring system and a -lactone ring. It contributed 60–90% of the inhibitory activity released from the roots of this tropical grass. Unlike nitrapyrin (a synthetic nitrification inhibitor), which affects only the ammonia monooxygenase (AMO) pathway, brachialactone appears to block both AMO and hydroxylamine oxidoreductase enzymatic pathways in Nitrosomonas. Release of this inhibitor is a regulated plant function, triggered and sustained by the availability of ammonium (NH4 ) in the root environment. Brachialactone release is restricted to those roots that are directly exposed to NH4 . Within 3 years of establishment, Brachiaria pastures have suppressed soil nitrifier populations (determined as amoA genes; ammonia-oxidizing bacteria and ammonia-oxidizing archaea), along with nitrification and nitrous oxide emissions. These findings provide direct evidence for the existence and active regulation of a nitrification inhibitor (or inhibitors) release from tropical pasture root systems. Exploiting the BNI function could become a powerful strategy toward the development of low-nitrifying agronomic systems, benefiting both agriculture and the environment. 2009-10-13 2014-09-24T07:58:38Z 2014-09-24T07:58:38Z Journal Article https://hdl.handle.net/10568/42836 en Open Access National Academy of Sciences Subbaraoa, Guntur V.; Nakahara, K.; Hurtado, MP; Ono, H; Moreta Mejia, DE; Salcedo, AF; Yoshihashi, AT; Ishikawa, T; Ishitani, Manabu; Ohnishi, M; Yoshida, M; Rondón, M.A.; Rao, Idupulapati M.; Lascano, Carlos E.; Berry, W.L.; Ito, O (2009). Evidence for biological nitrification inhibition in Brachiaria pastures. PNAS 106(41):17302-17307. |
| spellingShingle | feed crops brachiaria humidicola climate change nitrous oxide nitrification nitrification inhibitors nitrogen cycle pastures cambio climático oxido nitroso nitrificación inhibidores de la nitrificacion ciclo del nitrógeno pastizales Subbaraoa, Guntur V. Nakahara, K. Hurtado, M.P. Ono, H Moreta Mejia, DE Salcedo, A.F. Yoshihashi, AT Ishikawa, T. Ishitani, Manabu Ohnishi, M Yoshida, M Rondón, Marco Antonio Rao, Idupulapati M. Lascano Aguilar, Carlos Eduardo Berry, W.L. Ito, O. Evidence for biological nitrification inhibition in Brachiaria pastures |
| title | Evidence for biological nitrification inhibition in Brachiaria pastures |
| title_full | Evidence for biological nitrification inhibition in Brachiaria pastures |
| title_fullStr | Evidence for biological nitrification inhibition in Brachiaria pastures |
| title_full_unstemmed | Evidence for biological nitrification inhibition in Brachiaria pastures |
| title_short | Evidence for biological nitrification inhibition in Brachiaria pastures |
| title_sort | evidence for biological nitrification inhibition in brachiaria pastures |
| topic | feed crops brachiaria humidicola climate change nitrous oxide nitrification nitrification inhibitors nitrogen cycle pastures cambio climático oxido nitroso nitrificación inhibidores de la nitrificacion ciclo del nitrógeno pastizales |
| url | https://hdl.handle.net/10568/42836 |
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