Evaluation of three native entomopathogenic nematodes (Rhabditidae) against third instar larvae of Phyllophaga menetriesi (Coleoptera: Scarabaeidae)

The infectivity and pathogenicity of three native entomopathogenic nematodes (Heterorhabditis (HNI 0100 and sp-CIAT) and Steinernema (SNI 0198)) were evaluated in third instar larvae of Phyllophaga menetriesi (Blanchard) under laboratory conditions. Concentrations of 7000 and 13,000 infective juveni...

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Autores principales: Quintero Marín, M.P., Caicedo, AM, Montoya Lerma, J., Gaigl, A
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2006
Materias:
Acceso en línea:https://hdl.handle.net/10568/42835
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author Quintero Marín, M.P.
Caicedo, AM
Montoya Lerma, J.
Gaigl, A
author_browse Caicedo, AM
Gaigl, A
Montoya Lerma, J.
Quintero Marín, M.P.
author_facet Quintero Marín, M.P.
Caicedo, AM
Montoya Lerma, J.
Gaigl, A
author_sort Quintero Marín, M.P.
collection Repository of Agricultural Research Outputs (CGSpace)
description The infectivity and pathogenicity of three native entomopathogenic nematodes (Heterorhabditis (HNI 0100 and sp-CIAT) and Steinernema (SNI 0198)) were evaluated in third instar larvae of Phyllophaga menetriesi (Blanchard) under laboratory conditions. Concentrations of 7000 and 13,000 infective juveniles per millilitre were applied to the coleopteran larval host (individuals) that had been placed in plastic cups with 40 g of sterile sand at 5.8% (w/w) moisture. The evaluations were done 5 and 10 days after treatment. It was observed that both the percentage of infectivity (74.5%, n = 247), measured in terms of percentage of penetration and the percentage of pathogenicity (10.5%, n = 247) of the three native nematodes were significantly different due to the treatments. Steinernema had the highest values of infectivity (>80%), but no pathogenicity. In contrast, the treatments with Heterorhabditis, despite having the lowest degree of infectivity (52.9%), had the highest mortality values (31.6%). Therefore, the Heterorhabditis strains were considered to be the most promising as biological control organisms within an integrated pest management programme due to their higher degree of pathogenicity. Nevertheless, different strains and species of native nematodes should be evaluated in all the developmental stages of the pest.
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spelling CGSpace428352024-05-01T08:20:00Z Evaluation of three native entomopathogenic nematodes (Rhabditidae) against third instar larvae of Phyllophaga menetriesi (Coleoptera: Scarabaeidae) Quintero Marín, M.P. Caicedo, AM Montoya Lerma, J. Gaigl, A entomophilic nematodes biological control phyllophaga menetriesi nematodos entomófilos control biológico The infectivity and pathogenicity of three native entomopathogenic nematodes (Heterorhabditis (HNI 0100 and sp-CIAT) and Steinernema (SNI 0198)) were evaluated in third instar larvae of Phyllophaga menetriesi (Blanchard) under laboratory conditions. Concentrations of 7000 and 13,000 infective juveniles per millilitre were applied to the coleopteran larval host (individuals) that had been placed in plastic cups with 40 g of sterile sand at 5.8% (w/w) moisture. The evaluations were done 5 and 10 days after treatment. It was observed that both the percentage of infectivity (74.5%, n = 247), measured in terms of percentage of penetration and the percentage of pathogenicity (10.5%, n = 247) of the three native nematodes were significantly different due to the treatments. Steinernema had the highest values of infectivity (>80%), but no pathogenicity. In contrast, the treatments with Heterorhabditis, despite having the lowest degree of infectivity (52.9%), had the highest mortality values (31.6%). Therefore, the Heterorhabditis strains were considered to be the most promising as biological control organisms within an integrated pest management programme due to their higher degree of pathogenicity. Nevertheless, different strains and species of native nematodes should be evaluated in all the developmental stages of the pest. 2006-12 2014-09-24T07:58:38Z 2014-09-24T07:58:38Z Journal Article https://hdl.handle.net/10568/42835 en Open Access Springer
spellingShingle entomophilic nematodes
biological control
phyllophaga menetriesi
nematodos entomófilos
control biológico
Quintero Marín, M.P.
Caicedo, AM
Montoya Lerma, J.
Gaigl, A
Evaluation of three native entomopathogenic nematodes (Rhabditidae) against third instar larvae of Phyllophaga menetriesi (Coleoptera: Scarabaeidae)
title Evaluation of three native entomopathogenic nematodes (Rhabditidae) against third instar larvae of Phyllophaga menetriesi (Coleoptera: Scarabaeidae)
title_full Evaluation of three native entomopathogenic nematodes (Rhabditidae) against third instar larvae of Phyllophaga menetriesi (Coleoptera: Scarabaeidae)
title_fullStr Evaluation of three native entomopathogenic nematodes (Rhabditidae) against third instar larvae of Phyllophaga menetriesi (Coleoptera: Scarabaeidae)
title_full_unstemmed Evaluation of three native entomopathogenic nematodes (Rhabditidae) against third instar larvae of Phyllophaga menetriesi (Coleoptera: Scarabaeidae)
title_short Evaluation of three native entomopathogenic nematodes (Rhabditidae) against third instar larvae of Phyllophaga menetriesi (Coleoptera: Scarabaeidae)
title_sort evaluation of three native entomopathogenic nematodes rhabditidae against third instar larvae of phyllophaga menetriesi coleoptera scarabaeidae
topic entomophilic nematodes
biological control
phyllophaga menetriesi
nematodos entomófilos
control biológico
url https://hdl.handle.net/10568/42835
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