Discovery of an amylose-free starch mutant in cassava (Manihot esculenta Crantz)
One of the objectives of the cassava-breeding project at CIAT is the identification of clones with special root quality characteristics. A large number of self-pollinations have been made in search of useful recessive traits. During 2006 harvests an S1 plant produced roots that stained brownish-red...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
American Chemical Society
2007
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/42743 |
| _version_ | 1855536699610759168 |
|---|---|
| author | Ceballos, H. Sánchez, T. Morante, N Fregene, Martin A. Dufour, D.L. Smith, A.M. Denyer, K Pérez Velásquez, Juan Carlos Calle, F. Mestres, C. |
| author_browse | Calle, F. Ceballos, H. Denyer, K Dufour, D.L. Fregene, Martin A. Mestres, C. Morante, N Pérez Velásquez, Juan Carlos Smith, A.M. Sánchez, T. |
| author_facet | Ceballos, H. Sánchez, T. Morante, N Fregene, Martin A. Dufour, D.L. Smith, A.M. Denyer, K Pérez Velásquez, Juan Carlos Calle, F. Mestres, C. |
| author_sort | Ceballos, H. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | One of the objectives of the cassava-breeding project at CIAT is the identification of clones with special root quality characteristics. A large number of self-pollinations have been made in search of useful recessive traits. During 2006 harvests an S1 plant produced roots that stained brownish-red when treated with an iodine solution, suggesting that it had lower-than-normal levels of amylose in its starch. Colorimetric and DSC measurements indicated low levels (3.4%) and an absence of amylose in the starch, respectively. SDS-PAGE demonstrated the absence of GBSS enzyme in the starch from these roots. Pasting behavior was analyzed with a rapid visco-analyzer and resulted in larger values for peak viscosity, gel breakdown, and setback in the mutant compared with normal cassava starch. Solubility was considerably reduced, while the swelling index and volume fraction of the dispersed phase were higher in the mutant. No change in starch granule size or shape was observed. This is the first report of a natural mutation in cassava that drastically reduces amylose content in root starch. |
| format | Journal Article |
| id | CGSpace42743 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2007 |
| publishDateRange | 2007 |
| publishDateSort | 2007 |
| publisher | American Chemical Society |
| publisherStr | American Chemical Society |
| record_format | dspace |
| spelling | CGSpace427432024-03-06T10:16:43Z Discovery of an amylose-free starch mutant in cassava (Manihot esculenta Crantz) Ceballos, H. Sánchez, T. Morante, N Fregene, Martin A. Dufour, D.L. Smith, A.M. Denyer, K Pérez Velásquez, Juan Carlos Calle, F. Mestres, C. manihot esculenta genotypes starch amylose genetic variation plant breeding genotipos almidón amilasas variación genética fitomejoramiento One of the objectives of the cassava-breeding project at CIAT is the identification of clones with special root quality characteristics. A large number of self-pollinations have been made in search of useful recessive traits. During 2006 harvests an S1 plant produced roots that stained brownish-red when treated with an iodine solution, suggesting that it had lower-than-normal levels of amylose in its starch. Colorimetric and DSC measurements indicated low levels (3.4%) and an absence of amylose in the starch, respectively. SDS-PAGE demonstrated the absence of GBSS enzyme in the starch from these roots. Pasting behavior was analyzed with a rapid visco-analyzer and resulted in larger values for peak viscosity, gel breakdown, and setback in the mutant compared with normal cassava starch. Solubility was considerably reduced, while the swelling index and volume fraction of the dispersed phase were higher in the mutant. No change in starch granule size or shape was observed. This is the first report of a natural mutation in cassava that drastically reduces amylose content in root starch. 2007-09-01 2014-09-24T07:58:30Z 2014-09-24T07:58:30Z Journal Article https://hdl.handle.net/10568/42743 en Open Access American Chemical Society Ceballos, Hernán; Sánchez, Theresa; Morante, Nelson; Fregene, Martin; Dufour, Dominique; Smith, Alison M; Denyer, Kay; Pérez, Juan Carlos; Calle, Fernando; Mestres, Christian. 2007. Discovery of an Amylose-free Starch Mutant in Cassava (Manihot esculenta Crantz). Journal of Agricultural and Food Chemistry 55(18) 7469–7476. |
| spellingShingle | manihot esculenta genotypes starch amylose genetic variation plant breeding genotipos almidón amilasas variación genética fitomejoramiento Ceballos, H. Sánchez, T. Morante, N Fregene, Martin A. Dufour, D.L. Smith, A.M. Denyer, K Pérez Velásquez, Juan Carlos Calle, F. Mestres, C. Discovery of an amylose-free starch mutant in cassava (Manihot esculenta Crantz) |
| title | Discovery of an amylose-free starch mutant in cassava (Manihot esculenta Crantz) |
| title_full | Discovery of an amylose-free starch mutant in cassava (Manihot esculenta Crantz) |
| title_fullStr | Discovery of an amylose-free starch mutant in cassava (Manihot esculenta Crantz) |
| title_full_unstemmed | Discovery of an amylose-free starch mutant in cassava (Manihot esculenta Crantz) |
| title_short | Discovery of an amylose-free starch mutant in cassava (Manihot esculenta Crantz) |
| title_sort | discovery of an amylose free starch mutant in cassava manihot esculenta crantz |
| topic | manihot esculenta genotypes starch amylose genetic variation plant breeding genotipos almidón amilasas variación genética fitomejoramiento |
| url | https://hdl.handle.net/10568/42743 |
| work_keys_str_mv | AT ceballosh discoveryofanamylosefreestarchmutantincassavamanihotesculentacrantz AT sanchezt discoveryofanamylosefreestarchmutantincassavamanihotesculentacrantz AT moranten discoveryofanamylosefreestarchmutantincassavamanihotesculentacrantz AT fregenemartina discoveryofanamylosefreestarchmutantincassavamanihotesculentacrantz AT dufourdl discoveryofanamylosefreestarchmutantincassavamanihotesculentacrantz AT smitham discoveryofanamylosefreestarchmutantincassavamanihotesculentacrantz AT denyerk discoveryofanamylosefreestarchmutantincassavamanihotesculentacrantz AT perezvelasquezjuancarlos discoveryofanamylosefreestarchmutantincassavamanihotesculentacrantz AT callef discoveryofanamylosefreestarchmutantincassavamanihotesculentacrantz AT mestresc discoveryofanamylosefreestarchmutantincassavamanihotesculentacrantz |