Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues
Processing of large numbers smaples of plant tissue samples for molecular mapping and gene tagging requires methods that are quick, simple, and cheap, and that eventually can be automated. Organic solvents used for DNA extraction can represent a significant proportion of the overall cost. In this st...
| Autores principales: | , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
1995
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/42738 |
| _version_ | 1855515281572495360 |
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| author | Chavez, Alba L. Vergara, CE Mayer, Jorge Edgard |
| author_browse | Chavez, Alba L. Mayer, Jorge Edgard Vergara, CE |
| author_facet | Chavez, Alba L. Vergara, CE Mayer, Jorge Edgard |
| author_sort | Chavez, Alba L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Processing of large numbers smaples of plant tissue samples for molecular mapping and gene tagging requires methods that are quick, simple, and cheap, and that eventually can be automated. Organic solvents used for DNA extraction can represent a significant proportion of the overall cost. In this study we examined dichloromethane as a replacement for chloroform to be used in combination with phenol. |
| format | Journal Article |
| id | CGSpace42738 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1995 |
| publishDateRange | 1995 |
| publishDateSort | 1995 |
| record_format | dspace |
| spelling | CGSpace427382025-11-12T05:53:17Z Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues Chavez, Alba L. Vergara, CE Mayer, Jorge Edgard plant tissues dna molecular genetics methods tejidos vegetales adn genética molecular métodos Processing of large numbers smaples of plant tissue samples for molecular mapping and gene tagging requires methods that are quick, simple, and cheap, and that eventually can be automated. Organic solvents used for DNA extraction can represent a significant proportion of the overall cost. In this study we examined dichloromethane as a replacement for chloroform to be used in combination with phenol. 1995 2014-09-24T07:58:29Z 2014-09-24T07:58:29Z Journal Article https://hdl.handle.net/10568/42738 en Open Access application/pdf |
| spellingShingle | plant tissues dna molecular genetics methods tejidos vegetales adn genética molecular métodos Chavez, Alba L. Vergara, CE Mayer, Jorge Edgard Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues |
| title | Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues |
| title_full | Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues |
| title_fullStr | Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues |
| title_full_unstemmed | Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues |
| title_short | Dichloromethane as an economic alternative to chloroform in the extraction of DNA from plant tissues |
| title_sort | dichloromethane as an economic alternative to chloroform in the extraction of dna from plant tissues |
| topic | plant tissues dna molecular genetics methods tejidos vegetales adn genética molecular métodos |
| url | https://hdl.handle.net/10568/42738 |
| work_keys_str_mv | AT chavezalbal dichloromethaneasaneconomicalternativetochloroformintheextractionofdnafromplanttissues AT vergarace dichloromethaneasaneconomicalternativetochloroformintheextractionofdnafromplanttissues AT mayerjorgeedgard dichloromethaneasaneconomicalternativetochloroformintheextractionofdnafromplanttissues |