Detection of the cassava bacterial blight pathogen, Xanthomonas axonopodis pv. manihotis, by polymerase chain reaction
Cassava bacterial blight, caused by Xanthomonas axonopodis pv. manihotis, is of significant concern wherever cassava is grown. The movement of infected, asymptomatic stems is a major means of pathogen dispersal. A reliable and sensitive diagnostic procedure is necessary for the safe movement of cass...
| Autores principales: | , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Scientific Societies
1998
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| Acceso en línea: | https://hdl.handle.net/10568/42726 |
| _version_ | 1855538019424010240 |
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| author | Verdier, Valerie M. Mosquera Cifuentes, Gloria Maria Assigbétsé, K. |
| author_browse | Assigbétsé, K. Mosquera Cifuentes, Gloria Maria Verdier, Valerie M. |
| author_facet | Verdier, Valerie M. Mosquera Cifuentes, Gloria Maria Assigbétsé, K. |
| author_sort | Verdier, Valerie M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava bacterial blight, caused by Xanthomonas axonopodis pv. manihotis, is of significant concern wherever cassava is grown. The movement of infected, asymptomatic stems is a major means of pathogen dispersal. A reliable and sensitive diagnostic procedure is necessary for the safe movement of cassava planting material. We used a cloned and sequenced pathogenicity gene of X. axonopodis pv. manihotis to develop a polymerase chain reaction (PCR) test for this pathogen. A set of primers directed the amplification of an 898-bp fragment in all 107 pathogenic strains of X. axonopodis pv. manihotis tested. PCR products were not observed when genomic DNA was tested for 27 strains of other xanthomonads, for saprophytic bacteria, or for five nonpathogenic strains of X. axonopodis pv. manihotis. The primers worked well for pathogen detection in direct PCR assays of X. axonopodis pv. manihotis colonies grown on liquid medium and in PCR assays of extracts from leaf and stem lesions. The minimum number of cells that could be detected from cassava stem and leaf lesions was 3 × 102 to 104 CFU/ml. The PCR assays proved to be relativyel sensitive and could become very useful in detecting the pathogen in cassava planting material. |
| format | Journal Article |
| id | CGSpace42726 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1998 |
| publishDateRange | 1998 |
| publishDateSort | 1998 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace427262024-08-27T10:37:02Z Detection of the cassava bacterial blight pathogen, Xanthomonas axonopodis pv. manihotis, by polymerase chain reaction Verdier, Valerie M. Mosquera Cifuentes, Gloria Maria Assigbétsé, K. manihot esculenta xanthomonas pcr adn pathology dna patología Cassava bacterial blight, caused by Xanthomonas axonopodis pv. manihotis, is of significant concern wherever cassava is grown. The movement of infected, asymptomatic stems is a major means of pathogen dispersal. A reliable and sensitive diagnostic procedure is necessary for the safe movement of cassava planting material. We used a cloned and sequenced pathogenicity gene of X. axonopodis pv. manihotis to develop a polymerase chain reaction (PCR) test for this pathogen. A set of primers directed the amplification of an 898-bp fragment in all 107 pathogenic strains of X. axonopodis pv. manihotis tested. PCR products were not observed when genomic DNA was tested for 27 strains of other xanthomonads, for saprophytic bacteria, or for five nonpathogenic strains of X. axonopodis pv. manihotis. The primers worked well for pathogen detection in direct PCR assays of X. axonopodis pv. manihotis colonies grown on liquid medium and in PCR assays of extracts from leaf and stem lesions. The minimum number of cells that could be detected from cassava stem and leaf lesions was 3 × 102 to 104 CFU/ml. The PCR assays proved to be relativyel sensitive and could become very useful in detecting the pathogen in cassava planting material. 1998-01 2014-09-24T07:58:28Z 2014-09-24T07:58:28Z Journal Article https://hdl.handle.net/10568/42726 en Open Access Scientific Societies |
| spellingShingle | manihot esculenta xanthomonas pcr adn pathology dna patología Verdier, Valerie M. Mosquera Cifuentes, Gloria Maria Assigbétsé, K. Detection of the cassava bacterial blight pathogen, Xanthomonas axonopodis pv. manihotis, by polymerase chain reaction |
| title | Detection of the cassava bacterial blight pathogen, Xanthomonas axonopodis pv. manihotis, by polymerase chain reaction |
| title_full | Detection of the cassava bacterial blight pathogen, Xanthomonas axonopodis pv. manihotis, by polymerase chain reaction |
| title_fullStr | Detection of the cassava bacterial blight pathogen, Xanthomonas axonopodis pv. manihotis, by polymerase chain reaction |
| title_full_unstemmed | Detection of the cassava bacterial blight pathogen, Xanthomonas axonopodis pv. manihotis, by polymerase chain reaction |
| title_short | Detection of the cassava bacterial blight pathogen, Xanthomonas axonopodis pv. manihotis, by polymerase chain reaction |
| title_sort | detection of the cassava bacterial blight pathogen xanthomonas axonopodis pv manihotis by polymerase chain reaction |
| topic | manihot esculenta xanthomonas pcr adn pathology dna patología |
| url | https://hdl.handle.net/10568/42726 |
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