Detection of a strain of soybean mosaic virus affecting tropical forage species of Centrosema

A filamentous virus about 715 nm long was isolated from a plant of the tropical forage legume Centrosema macrocarpum affected by mosaic. Ultrathin sections of symptomatic leaf tissue showed pinwheel inclusions in the cytoplasm of infected cells. The virus was transmitted by mechanical means, by aphi...

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Main Authors: Morales, Francisco José, Niessen, AI, Castaño, M., Calvert, Lee A.
Format: Journal Article
Language:Inglés
Published: Scientific Societies 1990
Subjects:
Online Access:https://hdl.handle.net/10568/42725
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author Morales, Francisco José
Niessen, AI
Castaño, M.
Calvert, Lee A.
author_browse Calvert, Lee A.
Castaño, M.
Morales, Francisco José
Niessen, AI
author_facet Morales, Francisco José
Niessen, AI
Castaño, M.
Calvert, Lee A.
author_sort Morales, Francisco José
collection Repository of Agricultural Research Outputs (CGSpace)
description A filamentous virus about 715 nm long was isolated from a plant of the tropical forage legume Centrosema macrocarpum affected by mosaic. Ultrathin sections of symptomatic leaf tissue showed pinwheel inclusions in the cytoplasm of infected cells. The virus was transmitted by mechanical means, by aphids, and through seed. Purified virus preparations had an absorbance ratio (A 260/280) of 1.13, contained a single protein of relative molecular weight 32,500, and had a 9-kb nucleic acid species. The virus was serologically indistinguishable from soybean mosaic virus and was related to bean common mosaic and watermelon mosaic-2 viruses. Based on the results of serological and pathogenicity tests, we conclude that the potyvirus isolated from C. macrocarpum is a strain of soybean mosaic virus.
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spelling CGSpace427252024-04-25T06:00:18Z Detection of a strain of soybean mosaic virus affecting tropical forage species of Centrosema Morales, Francisco José Niessen, AI Castaño, M. Calvert, Lee A. feed crops centrosema macrocarpum virus del mosaico de la soya cepas sintomatologia rango de hospedantes centrosema entomología fitopatología leguminosas nematologia A filamentous virus about 715 nm long was isolated from a plant of the tropical forage legume Centrosema macrocarpum affected by mosaic. Ultrathin sections of symptomatic leaf tissue showed pinwheel inclusions in the cytoplasm of infected cells. The virus was transmitted by mechanical means, by aphids, and through seed. Purified virus preparations had an absorbance ratio (A 260/280) of 1.13, contained a single protein of relative molecular weight 32,500, and had a 9-kb nucleic acid species. The virus was serologically indistinguishable from soybean mosaic virus and was related to bean common mosaic and watermelon mosaic-2 viruses. Based on the results of serological and pathogenicity tests, we conclude that the potyvirus isolated from C. macrocarpum is a strain of soybean mosaic virus. 1990 2014-09-24T07:58:28Z 2014-09-24T07:58:28Z Journal Article https://hdl.handle.net/10568/42725 en Open Access Scientific Societies
spellingShingle feed crops
centrosema macrocarpum
virus del mosaico de la soya
cepas
sintomatologia
rango de hospedantes
centrosema
entomología
fitopatología
leguminosas
nematologia
Morales, Francisco José
Niessen, AI
Castaño, M.
Calvert, Lee A.
Detection of a strain of soybean mosaic virus affecting tropical forage species of Centrosema
title Detection of a strain of soybean mosaic virus affecting tropical forage species of Centrosema
title_full Detection of a strain of soybean mosaic virus affecting tropical forage species of Centrosema
title_fullStr Detection of a strain of soybean mosaic virus affecting tropical forage species of Centrosema
title_full_unstemmed Detection of a strain of soybean mosaic virus affecting tropical forage species of Centrosema
title_short Detection of a strain of soybean mosaic virus affecting tropical forage species of Centrosema
title_sort detection of a strain of soybean mosaic virus affecting tropical forage species of centrosema
topic feed crops
centrosema macrocarpum
virus del mosaico de la soya
cepas
sintomatologia
rango de hospedantes
centrosema
entomología
fitopatología
leguminosas
nematologia
url https://hdl.handle.net/10568/42725
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AT niessenai detectionofastrainofsoybeanmosaicvirusaffectingtropicalforagespeciesofcentrosema
AT castanom detectionofastrainofsoybeanmosaicvirusaffectingtropicalforagespeciesofcentrosema
AT calvertleea detectionofastrainofsoybeanmosaicvirusaffectingtropicalforagespeciesofcentrosema