Analyses of genetic diversity in Cuban rice varieties using isozyme, RAPD and AFLP markers
A survey of the genetic diversity among the major cuban rice cultivars was conducted using isozyme, RAPD and AFLP markers. Polymorphisms were detected for esterases, peroxidases, alcohol dehydrogenases and polyphenoloxidases systems; 21 RAPD primers and four AFLP primer combinations. Heterozygosity...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
1999
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/42581 |
| _version_ | 1855542430977228800 |
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| author | Fuentes, J.L Escobar, F. Álvarez, A. Gallego Sánchez, Gerardo J. Duque E., Myriam Cristina Ferrer, M Deus, J.E. Tohme, Joseph M. |
| author_browse | Deus, J.E. Duque E., Myriam Cristina Escobar, F. Ferrer, M Fuentes, J.L Gallego Sánchez, Gerardo J. Tohme, Joseph M. Álvarez, A. |
| author_facet | Fuentes, J.L Escobar, F. Álvarez, A. Gallego Sánchez, Gerardo J. Duque E., Myriam Cristina Ferrer, M Deus, J.E. Tohme, Joseph M. |
| author_sort | Fuentes, J.L |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A survey of the genetic diversity among the major cuban rice cultivars was conducted using isozyme, RAPD and AFLP markers. Polymorphisms were detected for esterases, peroxidases, alcohol dehydrogenases and polyphenoloxidases systems; 21 RAPD primers and four AFLP primer combinations. Heterozygosity arithmetic mean value (Hav(p)), the effective multiplex ratio (EMR) and the marker index (MI), were calculated for isozyme, RAPD and AFLP markers. The mean value of genetic similarity among the different varieties was 0.92 for isozyme, 0.73 for RAPD and 0.58 for AFLP analyses. Thus, AFLP were able to detect polymorphisms with higher efficiency than RAPD (+15%) and isozyme (+34%). Data from the isozyme, RAPD and AFLP analyses were used to compute matrices of genetic similarities. The efficiency of the UPGMA for the estimation of genetic relatedness among varieties was supported by cophenetic correlation coefficients. The resulting values indicated that the distortion level for the estimated similarities was minimal. The correlation coefficients obtained by the Mantel matrix correspondence test, which was used to compare the cophenetic matrices for the different markers, showed that estimated values of genetic relationship given for isozyme and RAPD markers (r = 0.89), as well as for AFLP and RAPD markers (r = 0.82) were properly related. However, AFLP and isozyme data showed only moderate correlation (r = 0.63). Although the genetic variability found among the different cultivars was low, both RAPD and AFLP markers proved to be efficient tools in assessing the genetic diversity of rice genotypes. |
| format | Journal Article |
| id | CGSpace42581 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1999 |
| publishDateRange | 1999 |
| publishDateSort | 1999 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace425812024-08-29T11:41:37Z Analyses of genetic diversity in Cuban rice varieties using isozyme, RAPD and AFLP markers Fuentes, J.L Escobar, F. Álvarez, A. Gallego Sánchez, Gerardo J. Duque E., Myriam Cristina Ferrer, M Deus, J.E. Tohme, Joseph M. oryza sativa genetic markers dna fingerprinting isoenzymes rice marcadores genéticos huellas genéticas adn isoenzimas arroz A survey of the genetic diversity among the major cuban rice cultivars was conducted using isozyme, RAPD and AFLP markers. Polymorphisms were detected for esterases, peroxidases, alcohol dehydrogenases and polyphenoloxidases systems; 21 RAPD primers and four AFLP primer combinations. Heterozygosity arithmetic mean value (Hav(p)), the effective multiplex ratio (EMR) and the marker index (MI), were calculated for isozyme, RAPD and AFLP markers. The mean value of genetic similarity among the different varieties was 0.92 for isozyme, 0.73 for RAPD and 0.58 for AFLP analyses. Thus, AFLP were able to detect polymorphisms with higher efficiency than RAPD (+15%) and isozyme (+34%). Data from the isozyme, RAPD and AFLP analyses were used to compute matrices of genetic similarities. The efficiency of the UPGMA for the estimation of genetic relatedness among varieties was supported by cophenetic correlation coefficients. The resulting values indicated that the distortion level for the estimated similarities was minimal. The correlation coefficients obtained by the Mantel matrix correspondence test, which was used to compare the cophenetic matrices for the different markers, showed that estimated values of genetic relationship given for isozyme and RAPD markers (r = 0.89), as well as for AFLP and RAPD markers (r = 0.82) were properly related. However, AFLP and isozyme data showed only moderate correlation (r = 0.63). Although the genetic variability found among the different cultivars was low, both RAPD and AFLP markers proved to be efficient tools in assessing the genetic diversity of rice genotypes. 1999 2014-09-24T07:58:14Z 2014-09-24T07:58:14Z Journal Article https://hdl.handle.net/10568/42581 en Limited Access Springer |
| spellingShingle | oryza sativa genetic markers dna fingerprinting isoenzymes rice marcadores genéticos huellas genéticas adn isoenzimas arroz Fuentes, J.L Escobar, F. Álvarez, A. Gallego Sánchez, Gerardo J. Duque E., Myriam Cristina Ferrer, M Deus, J.E. Tohme, Joseph M. Analyses of genetic diversity in Cuban rice varieties using isozyme, RAPD and AFLP markers |
| title | Analyses of genetic diversity in Cuban rice varieties using isozyme, RAPD and AFLP markers |
| title_full | Analyses of genetic diversity in Cuban rice varieties using isozyme, RAPD and AFLP markers |
| title_fullStr | Analyses of genetic diversity in Cuban rice varieties using isozyme, RAPD and AFLP markers |
| title_full_unstemmed | Analyses of genetic diversity in Cuban rice varieties using isozyme, RAPD and AFLP markers |
| title_short | Analyses of genetic diversity in Cuban rice varieties using isozyme, RAPD and AFLP markers |
| title_sort | analyses of genetic diversity in cuban rice varieties using isozyme rapd and aflp markers |
| topic | oryza sativa genetic markers dna fingerprinting isoenzymes rice marcadores genéticos huellas genéticas adn isoenzimas arroz |
| url | https://hdl.handle.net/10568/42581 |
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