AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis
Summary: Xanthomonas axonopodis pv. manihotis (Xam) is the causative agent of cassava bacterial blight (CBB), a worldwide disease that is particularly destructive in South America and Africa. CBB is controlled essentially through the use of resistant varieties. To develop an appropriate disease mana...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
1999
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/42558 |
| _version_ | 1855528987077378048 |
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| author | Restrepo, S. Duque E., Myriam Cristina Tohme, Joseph M. Verdier, Valerie M. |
| author_browse | Duque E., Myriam Cristina Restrepo, S. Tohme, Joseph M. Verdier, Valerie M. |
| author_facet | Restrepo, S. Duque E., Myriam Cristina Tohme, Joseph M. Verdier, Valerie M. |
| author_sort | Restrepo, S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Summary: Xanthomonas axonopodis pv. manihotis (Xam) is the causative agent of cassava bacterial blight (CBB), a worldwide disease that is particularly destructive in South America and Africa. CBB is controlled essentially through the use of resistant varieties. To develop an appropriate disease management strategy, the genetic diversity of the pathogen's populations must be assessed. Until now, the genetic diversity of Xam was characterized by RFLP analyses using ribotyping, and plasmid and genomic Xam probes. We used AFLP (amplified fragment length polymorphism), a novel PCR-based technique, to characterize the genetic diversity of Colombian Xam isolates. Six Xam strains were tested with 65 AFLP primer combinations to identify the best selective primers. Eight primer combinations were selected according to their reproducibility, number of polymorphic bands and polymorphism detected between Xam strains. Forty-seven Xam strains, originating from different Colombian ecozones, were analysed with the selected combinations. Results obtained with AFLP are consistent with those obtained with RFLP, using plasmid DNA as a probe. Some primer combinations differentiated Xam strains that were not distinguished by RFLP analyses, thus AFLP fingerprinting allowed a better definition of the genetic relationships between Xam strains. |
| format | Journal Article |
| id | CGSpace42558 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1999 |
| publishDateRange | 1999 |
| publishDateSort | 1999 |
| record_format | dspace |
| spelling | CGSpace425582023-02-15T05:16:49Z AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis Restrepo, S. Duque E., Myriam Cristina Tohme, Joseph M. Verdier, Valerie M. manihot esculenta xanthomonas axonopodis genetic markers dna fingerprinting dna genetic variation marcadores genéticos huellas genéticas adn adn variación genética Summary: Xanthomonas axonopodis pv. manihotis (Xam) is the causative agent of cassava bacterial blight (CBB), a worldwide disease that is particularly destructive in South America and Africa. CBB is controlled essentially through the use of resistant varieties. To develop an appropriate disease management strategy, the genetic diversity of the pathogen's populations must be assessed. Until now, the genetic diversity of Xam was characterized by RFLP analyses using ribotyping, and plasmid and genomic Xam probes. We used AFLP (amplified fragment length polymorphism), a novel PCR-based technique, to characterize the genetic diversity of Colombian Xam isolates. Six Xam strains were tested with 65 AFLP primer combinations to identify the best selective primers. Eight primer combinations were selected according to their reproducibility, number of polymorphic bands and polymorphism detected between Xam strains. Forty-seven Xam strains, originating from different Colombian ecozones, were analysed with the selected combinations. Results obtained with AFLP are consistent with those obtained with RFLP, using plasmid DNA as a probe. Some primer combinations differentiated Xam strains that were not distinguished by RFLP analyses, thus AFLP fingerprinting allowed a better definition of the genetic relationships between Xam strains. 1999 2014-09-24T07:58:12Z 2014-09-24T07:58:12Z Journal Article https://hdl.handle.net/10568/42558 en Open Access |
| spellingShingle | manihot esculenta xanthomonas axonopodis genetic markers dna fingerprinting dna genetic variation marcadores genéticos huellas genéticas adn adn variación genética Restrepo, S. Duque E., Myriam Cristina Tohme, Joseph M. Verdier, Valerie M. AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis |
| title | AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis |
| title_full | AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis |
| title_fullStr | AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis |
| title_full_unstemmed | AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis |
| title_short | AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis |
| title_sort | aflp fingerprinting an efficient technique for detecting genetic variation of xanthomonas axonopodis pv manihotis |
| topic | manihot esculenta xanthomonas axonopodis genetic markers dna fingerprinting dna genetic variation marcadores genéticos huellas genéticas adn adn variación genética |
| url | https://hdl.handle.net/10568/42558 |
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