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Acid PAGE procedure for detection of phaseolins in field beans (Phaseolus vulgaris L.)

A procedure based on acid polyacrylamide gel electrophoresis (acid PAGE) has been described. Resolution of phaseolin bands appears to be relatively superior to that obtained with basic PAGE system. The procedure discriminates S, T, C, M and B types of phaseolins; S, T and C have been reported previo...

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Main Authors: Hussain, A., Espinel, MT, Roca, W., Bushhuk, W
Format: Journal Article
Language:Inglés
Published: Springer 1989
Subjects:
phaseolus vulgaris
proteins
analysis
electrophoresis
phaseolin
phytoalexins
faseolina
proteinas
análisis
electroforesis
Online Access:https://hdl.handle.net/10568/42545
Summary:A procedure based on acid polyacrylamide gel electrophoresis (acid PAGE) has been described. Resolution of phaseolin bands appears to be relatively superior to that obtained with basic PAGE system. The procedure discriminates S, T, C, M and B types of phaseolins; S, T and C have been reported previously while M and B types were identified in the present study.

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