A strain of soybean mosaic virus infecting Passiflora spp. in Colombia
Viruses causing severe mosaic, epinasty, defoliation, and premature death of Passiflora spp. were isolated from west central Colombia. Western blot analyses indicated the presence of potyviruses. Two Colombian isolates (COL-22 and -GR) were compared to known potyviruses as to host range, symptomatol...
| Autores principales: | , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Scientific Societies
1996
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/42539 |
| _version_ | 1855515143629176832 |
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| author | Benscher, D Pappu, SS Niblett, CL Varón de Agudelo, Francia Morales, Francisco José Hodson de Jaramillo, E Álvarez, E. Acosta, O. |
| author_browse | Acosta, O. Benscher, D Hodson de Jaramillo, E Morales, Francisco José Niblett, CL Pappu, SS Varón de Agudelo, Francia Álvarez, E. |
| author_facet | Benscher, D Pappu, SS Niblett, CL Varón de Agudelo, Francia Morales, Francisco José Hodson de Jaramillo, E Álvarez, E. Acosta, O. |
| author_sort | Benscher, D |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Viruses causing severe mosaic, epinasty, defoliation, and premature death of Passiflora spp. were isolated from west central Colombia. Western blot analyses indicated the presence of potyviruses. Two Colombian isolates (COL-22 and -GR) were compared to known potyviruses as to host range, symptomatology, serological activity, amino acid and nucleotide sequence similarity in the coat protein and 3' noncoding region (3'NCR), and reactivity in dot-blot hybridization. Host range and symptomatology of COL-22 and -GR indicated they are similar to soybean mosaic virus (SMV). Also, both isolates reacted strongly with SMV antisera in immunodiffusion plates. Aphid transmission by Aphis gossypii and Toxoptera citricida was confirmed, but seed transmission was not demonstrated. In addition to COL-22 and -GR, five other Colombian isolates were cloned and sequenced. The deduced coat protein amino acid sequences for all of the Colombian isolates were virtually identical and shared a 98% similarity with SMV. Dot-blot hybridization experiments, using DNA complementary to the 3'NCR of the virus as a probe, further confirmed that the potyvirus infecting Passiflora spp. in Colombia is a strain of SMV. To our knowledge, infection of Passiflora spp. by a strain of SMV has not been previously reported. |
| format | Journal Article |
| id | CGSpace42539 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1996 |
| publishDateRange | 1996 |
| publishDateSort | 1996 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace425392024-04-25T06:00:18Z A strain of soybean mosaic virus infecting Passiflora spp. in Colombia Benscher, D Pappu, SS Niblett, CL Varón de Agudelo, Francia Morales, Francisco José Hodson de Jaramillo, E Álvarez, E. Acosta, O. passiflora viroses soybean mosaic potyvirus molecular genetics vectors aphis gossypii toxoptera citricidus diagnosis virosis potyvirus del mosaico de la soja genética molecular vectores diagnóstico Viruses causing severe mosaic, epinasty, defoliation, and premature death of Passiflora spp. were isolated from west central Colombia. Western blot analyses indicated the presence of potyviruses. Two Colombian isolates (COL-22 and -GR) were compared to known potyviruses as to host range, symptomatology, serological activity, amino acid and nucleotide sequence similarity in the coat protein and 3' noncoding region (3'NCR), and reactivity in dot-blot hybridization. Host range and symptomatology of COL-22 and -GR indicated they are similar to soybean mosaic virus (SMV). Also, both isolates reacted strongly with SMV antisera in immunodiffusion plates. Aphid transmission by Aphis gossypii and Toxoptera citricida was confirmed, but seed transmission was not demonstrated. In addition to COL-22 and -GR, five other Colombian isolates were cloned and sequenced. The deduced coat protein amino acid sequences for all of the Colombian isolates were virtually identical and shared a 98% similarity with SMV. Dot-blot hybridization experiments, using DNA complementary to the 3'NCR of the virus as a probe, further confirmed that the potyvirus infecting Passiflora spp. in Colombia is a strain of SMV. To our knowledge, infection of Passiflora spp. by a strain of SMV has not been previously reported. 1996 2014-09-24T07:58:10Z 2014-09-24T07:58:10Z Journal Article https://hdl.handle.net/10568/42539 en Open Access Scientific Societies |
| spellingShingle | passiflora viroses soybean mosaic potyvirus molecular genetics vectors aphis gossypii toxoptera citricidus diagnosis virosis potyvirus del mosaico de la soja genética molecular vectores diagnóstico Benscher, D Pappu, SS Niblett, CL Varón de Agudelo, Francia Morales, Francisco José Hodson de Jaramillo, E Álvarez, E. Acosta, O. A strain of soybean mosaic virus infecting Passiflora spp. in Colombia |
| title | A strain of soybean mosaic virus infecting Passiflora spp. in Colombia |
| title_full | A strain of soybean mosaic virus infecting Passiflora spp. in Colombia |
| title_fullStr | A strain of soybean mosaic virus infecting Passiflora spp. in Colombia |
| title_full_unstemmed | A strain of soybean mosaic virus infecting Passiflora spp. in Colombia |
| title_short | A strain of soybean mosaic virus infecting Passiflora spp. in Colombia |
| title_sort | strain of soybean mosaic virus infecting passiflora spp in colombia |
| topic | passiflora viroses soybean mosaic potyvirus molecular genetics vectors aphis gossypii toxoptera citricidus diagnosis virosis potyvirus del mosaico de la soja genética molecular vectores diagnóstico |
| url | https://hdl.handle.net/10568/42539 |
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