A reproducible genetic transformation system for cultivated Phaseolus acutifolius (tepary bean) and its use to assess the role of arcelins in resistance to the Mexican bean weevil
A reproducible Agrobacterium tumefaciens-mediated genetic transformation method that delivers fertile and morphologically normal transgenic plants was developed for cultivated tepary bean (Phaseolus acutifolius L. Gray). Factors contributing to higher transformation efficiencies include (1) a low in...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2005
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/42535 |
| _version_ | 1855535893264203776 |
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| author | Zambre, M Goossens, A Cardona Mejía, César Montagu, M. van Terryn, N Angenon, G |
| author_browse | Angenon, G Cardona Mejía, César Goossens, A Montagu, M. van Terryn, N Zambre, M |
| author_facet | Zambre, M Goossens, A Cardona Mejía, César Montagu, M. van Terryn, N Angenon, G |
| author_sort | Zambre, M |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A reproducible Agrobacterium tumefaciens-mediated genetic transformation method that delivers fertile and morphologically normal transgenic plants was developed for cultivated tepary bean (Phaseolus acutifolius L. Gray). Factors contributing to higher transformation efficiencies include (1) a low initial concentration of bacteria coupled with a longer cocultivation period with callus, (2) an initial selection of callus on a medium containing low levels of the selectable agent, (3) omission of the selectable agent from the medium during callus differentiation to shoots and (4) the efficient conversion of transgenic shoots into fertile plants. All plants regenerated with this procedure (T0) were stably transformed, and the introduced foreign genes were inherited in a Mendelian fashion in most of the 33 independent transformants. Integration, stable transmission and high expression levels of the transgenes were observed in the T1 and/or T3 progenies of the transgenic lines. The binary transformation vectors contained the ?-glucuronidase reporter gene, the neomycin phosphotransferase II selectable marker gene and either an arcelin 1 or an arcelin 5 gene. Arcelins are seed proteins that are very abundant in some wild P. vulgaris L. genotypes showing resistance to the storage insect Zabrotes subfasciatus (Boheman) (Coleoptera, Bruchidae). Transgenic beans from two different cultivated P. acutifolius genotypes with high arcelin levels were infested with Z. subfasciatus, but they were only marginally less susceptible to infestation than the non-transgenic P. acutifolius. Hence, the arcelin genes tested here are not major determinants of resistance against Z. subfasciatus. |
| format | Journal Article |
| id | CGSpace42535 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2005 |
| publishDateRange | 2005 |
| publishDateSort | 2005 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace425352024-08-27T10:36:50Z A reproducible genetic transformation system for cultivated Phaseolus acutifolius (tepary bean) and its use to assess the role of arcelins in resistance to the Mexican bean weevil Zambre, M Goossens, A Cardona Mejía, César Montagu, M. van Terryn, N Angenon, G phaseolus acutifolius zabrotes subfasciatus stored products pests agrobacterium tumefaciens transgenic plants genetic transformation pest resistance genetic resistance plagas de productos almacenados plantas transgénicas transformación genética resistencia a la enfermedad resistencia genética A reproducible Agrobacterium tumefaciens-mediated genetic transformation method that delivers fertile and morphologically normal transgenic plants was developed for cultivated tepary bean (Phaseolus acutifolius L. Gray). Factors contributing to higher transformation efficiencies include (1) a low initial concentration of bacteria coupled with a longer cocultivation period with callus, (2) an initial selection of callus on a medium containing low levels of the selectable agent, (3) omission of the selectable agent from the medium during callus differentiation to shoots and (4) the efficient conversion of transgenic shoots into fertile plants. All plants regenerated with this procedure (T0) were stably transformed, and the introduced foreign genes were inherited in a Mendelian fashion in most of the 33 independent transformants. Integration, stable transmission and high expression levels of the transgenes were observed in the T1 and/or T3 progenies of the transgenic lines. The binary transformation vectors contained the ?-glucuronidase reporter gene, the neomycin phosphotransferase II selectable marker gene and either an arcelin 1 or an arcelin 5 gene. Arcelins are seed proteins that are very abundant in some wild P. vulgaris L. genotypes showing resistance to the storage insect Zabrotes subfasciatus (Boheman) (Coleoptera, Bruchidae). Transgenic beans from two different cultivated P. acutifolius genotypes with high arcelin levels were infested with Z. subfasciatus, but they were only marginally less susceptible to infestation than the non-transgenic P. acutifolius. Hence, the arcelin genes tested here are not major determinants of resistance against Z. subfasciatus. 2005-03 2014-09-24T07:58:09Z 2014-09-24T07:58:09Z Journal Article https://hdl.handle.net/10568/42535 en Limited Access Springer |
| spellingShingle | phaseolus acutifolius zabrotes subfasciatus stored products pests agrobacterium tumefaciens transgenic plants genetic transformation pest resistance genetic resistance plagas de productos almacenados plantas transgénicas transformación genética resistencia a la enfermedad resistencia genética Zambre, M Goossens, A Cardona Mejía, César Montagu, M. van Terryn, N Angenon, G A reproducible genetic transformation system for cultivated Phaseolus acutifolius (tepary bean) and its use to assess the role of arcelins in resistance to the Mexican bean weevil |
| title | A reproducible genetic transformation system for cultivated Phaseolus acutifolius (tepary bean) and its use to assess the role of arcelins in resistance to the Mexican bean weevil |
| title_full | A reproducible genetic transformation system for cultivated Phaseolus acutifolius (tepary bean) and its use to assess the role of arcelins in resistance to the Mexican bean weevil |
| title_fullStr | A reproducible genetic transformation system for cultivated Phaseolus acutifolius (tepary bean) and its use to assess the role of arcelins in resistance to the Mexican bean weevil |
| title_full_unstemmed | A reproducible genetic transformation system for cultivated Phaseolus acutifolius (tepary bean) and its use to assess the role of arcelins in resistance to the Mexican bean weevil |
| title_short | A reproducible genetic transformation system for cultivated Phaseolus acutifolius (tepary bean) and its use to assess the role of arcelins in resistance to the Mexican bean weevil |
| title_sort | reproducible genetic transformation system for cultivated phaseolus acutifolius tepary bean and its use to assess the role of arcelins in resistance to the mexican bean weevil |
| topic | phaseolus acutifolius zabrotes subfasciatus stored products pests agrobacterium tumefaciens transgenic plants genetic transformation pest resistance genetic resistance plagas de productos almacenados plantas transgénicas transformación genética resistencia a la enfermedad resistencia genética |
| url | https://hdl.handle.net/10568/42535 |
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