Molecular characterization and identification of biocontrol isolates of Trichoderma harzianum from Embu District, Kenya
Species in the genus Trichoderma are important as commercial source of several enzymes, biofungicides, and growth promoters. The most common biological control agents of the genus are strains of T. harzianum, T .viride and T. viriens. In this study, sixteen selected isolates of T. harzianum from dif...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2011
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| Acceso en línea: | https://hdl.handle.net/10568/42503 |
| _version_ | 1855542354659770368 |
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| author | Siameto, EN Okoth, S. Amugune, N.O. Chege, NC |
| author_browse | Amugune, N.O. Chege, NC Okoth, S. Siameto, EN |
| author_facet | Siameto, EN Okoth, S. Amugune, N.O. Chege, NC |
| author_sort | Siameto, EN |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Species in the genus Trichoderma are important as commercial source of several enzymes, biofungicides, and growth promoters. The most common biological control agents of the genus are strains of T. harzianum, T .viride and T. viriens. In this study, sixteen selected isolates of T. harzianum from different land use types in Embu, Kenya were tested for anatogonistic action against five soil borne phytopathogenic fungi (Rhizoctonia solani, Pythium sp, Fusarium graminearum, F. oxysporum f. sp phaseoli and F. oxysporum f. sp Lycopersici) using dual culture assay and through production of non-volatile inhibitors. Seven isolates were further characterized using RAPD-PCR procedure to determine genetic variability. All T. harzianum isolates had considerable antagonistic effect on mycelial growth of the pathogens in dual cultures compared to the controls. Maximum inhibition occurred in Pythium sp-055E interaction (73%).The culture filtrates obtained from Czapek’s liquid medium reduced the dry weight (mg) of the mycelia significantly while those from the potato dextrose broth showed minimum inhibition growth. Pythium sp was inhibited the most compared to other pathogens. Genetic similarities generated using Jaccard’s coefficient of similarity ranged from 0.231 between isolates 055E and 011E to 0.857 between isolates 010E and 015E. The technique of RAPD was efficient in demonstrating the DNA polymorphism in the isolates of T. harzianum tested showing intraspecific genetic variability. Since all T. harzianum isolates evaluated were effective in controlling colony growth of the soil borne pathogens both in dual cultures and in culture filtrates they should be tried as a broad spectrum biological control agent in the greenhouse and under field conditions. |
| format | Journal Article |
| id | CGSpace42503 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2011 |
| publishDateRange | 2011 |
| publishDateSort | 2011 |
| record_format | dspace |
| spelling | CGSpace425032023-02-15T05:22:36Z Molecular characterization and identification of biocontrol isolates of Trichoderma harzianum from Embu District, Kenya Siameto, EN Okoth, S. Amugune, N.O. Chege, NC biological control trichoderma harzianum rhizoctonia fusarium pcr rapd Species in the genus Trichoderma are important as commercial source of several enzymes, biofungicides, and growth promoters. The most common biological control agents of the genus are strains of T. harzianum, T .viride and T. viriens. In this study, sixteen selected isolates of T. harzianum from different land use types in Embu, Kenya were tested for anatogonistic action against five soil borne phytopathogenic fungi (Rhizoctonia solani, Pythium sp, Fusarium graminearum, F. oxysporum f. sp phaseoli and F. oxysporum f. sp Lycopersici) using dual culture assay and through production of non-volatile inhibitors. Seven isolates were further characterized using RAPD-PCR procedure to determine genetic variability. All T. harzianum isolates had considerable antagonistic effect on mycelial growth of the pathogens in dual cultures compared to the controls. Maximum inhibition occurred in Pythium sp-055E interaction (73%).The culture filtrates obtained from Czapek’s liquid medium reduced the dry weight (mg) of the mycelia significantly while those from the potato dextrose broth showed minimum inhibition growth. Pythium sp was inhibited the most compared to other pathogens. Genetic similarities generated using Jaccard’s coefficient of similarity ranged from 0.231 between isolates 055E and 011E to 0.857 between isolates 010E and 015E. The technique of RAPD was efficient in demonstrating the DNA polymorphism in the isolates of T. harzianum tested showing intraspecific genetic variability. Since all T. harzianum isolates evaluated were effective in controlling colony growth of the soil borne pathogens both in dual cultures and in culture filtrates they should be tried as a broad spectrum biological control agent in the greenhouse and under field conditions. 2011 2014-09-24T07:58:05Z 2014-09-24T07:58:05Z Journal Article https://hdl.handle.net/10568/42503 en Open Access Siameto, Elizabeth, Sheila Okoth, Nelson Amugune, & N.C. Chege. "molecular characterization and identification of biocontrol isolates of trichoderma harzianum from Embu District, Kenya." Tropical and Subtropical Agroecosystems [Online], 13.1 (2011): 81-90. Web. 13 Jun. 2014 |
| spellingShingle | biological control trichoderma harzianum rhizoctonia fusarium pcr rapd Siameto, EN Okoth, S. Amugune, N.O. Chege, NC Molecular characterization and identification of biocontrol isolates of Trichoderma harzianum from Embu District, Kenya |
| title | Molecular characterization and identification of biocontrol isolates of Trichoderma harzianum from Embu District, Kenya |
| title_full | Molecular characterization and identification of biocontrol isolates of Trichoderma harzianum from Embu District, Kenya |
| title_fullStr | Molecular characterization and identification of biocontrol isolates of Trichoderma harzianum from Embu District, Kenya |
| title_full_unstemmed | Molecular characterization and identification of biocontrol isolates of Trichoderma harzianum from Embu District, Kenya |
| title_short | Molecular characterization and identification of biocontrol isolates of Trichoderma harzianum from Embu District, Kenya |
| title_sort | molecular characterization and identification of biocontrol isolates of trichoderma harzianum from embu district kenya |
| topic | biological control trichoderma harzianum rhizoctonia fusarium pcr rapd |
| url | https://hdl.handle.net/10568/42503 |
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