Abundance and diversity of legume nodulating rhizobia in soils of Embu district, Kenya
A major strategy towards addressing soil fertility depletion is the conservation and sustainable use of rhizobia that are able to fix nitrogen in the soil in association with legumes. The study assessed abundance and diversity of legume nodulating rhizobia (LNB) in soils collected from six different...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2011
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/42494 |
| _version_ | 1855523039981076480 |
|---|---|
| author | Mwenda, George M. Karanja, Nancy M. Boga, Hamadi M. Kahindi, James M. Muigai, A.M. Odee, D |
| author_browse | Boga, Hamadi M. Kahindi, James M. Karanja, Nancy M. Muigai, A.M. Mwenda, George M. Odee, D |
| author_facet | Mwenda, George M. Karanja, Nancy M. Boga, Hamadi M. Kahindi, James M. Muigai, A.M. Odee, D |
| author_sort | Mwenda, George M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A major strategy towards addressing soil fertility depletion is the conservation and sustainable use of rhizobia that are able to fix nitrogen in the soil in association with legumes. The study assessed abundance and diversity of legume nodulating rhizobia (LNB) in soils collected from six different land use systems in Embu District, Kenya. The populations were estimated by the most-probable-number (MPN) plant infection technique using Macroptilium atropurpureum (DC.) Urban (Siratro) as the trap host species. Symbiotic effectiveness was measured for the isolates in association with Siratro. Isolated rhizobia were characterized morphologically and genetically by PCR-RFLP and partial sequencing of 16S rRNA genes. The LNB populations in soils collected from the different land uses in Embu ranged from 0 to 2.3 ? 102 cells g-1 soil. There was apparent land use effect on abundance of LNB with fallow system giving high abundance. A total of 250 pure isolates were obtained from the root nodules of Siratro trap plants. The isolates were characterized on yeast extract mannitol mineral salts agar (YEMA) media containing bromothymol blue and grouped into fast growers (acid-producing) and slow growers (alkali-producing) (70% and 30 % of isolates respectively). PCR-RFLP analysis categorised the rhizobia into five species in the genera Rhizobium, Bradyrhizobium, Mesorhizobium and Agrobacterium. Land use system under tea had four of the five species found in the area whereas natural forests had two species. Land use significantly impacted on the diversity of rhizobia |
| format | Journal Article |
| id | CGSpace42494 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2011 |
| publishDateRange | 2011 |
| publishDateSort | 2011 |
| record_format | dspace |
| spelling | CGSpace424942023-02-15T05:14:06Z Abundance and diversity of legume nodulating rhizobia in soils of Embu district, Kenya Mwenda, George M. Karanja, Nancy M. Boga, Hamadi M. Kahindi, James M. Muigai, A.M. Odee, D land use cropping systems feed legumes rhizobium symbiosis soil fertility utilización de la tierra sistemas de cultivo leguminosas forrajeras simbiosis fertilidad del suelo A major strategy towards addressing soil fertility depletion is the conservation and sustainable use of rhizobia that are able to fix nitrogen in the soil in association with legumes. The study assessed abundance and diversity of legume nodulating rhizobia (LNB) in soils collected from six different land use systems in Embu District, Kenya. The populations were estimated by the most-probable-number (MPN) plant infection technique using Macroptilium atropurpureum (DC.) Urban (Siratro) as the trap host species. Symbiotic effectiveness was measured for the isolates in association with Siratro. Isolated rhizobia were characterized morphologically and genetically by PCR-RFLP and partial sequencing of 16S rRNA genes. The LNB populations in soils collected from the different land uses in Embu ranged from 0 to 2.3 ? 102 cells g-1 soil. There was apparent land use effect on abundance of LNB with fallow system giving high abundance. A total of 250 pure isolates were obtained from the root nodules of Siratro trap plants. The isolates were characterized on yeast extract mannitol mineral salts agar (YEMA) media containing bromothymol blue and grouped into fast growers (acid-producing) and slow growers (alkali-producing) (70% and 30 % of isolates respectively). PCR-RFLP analysis categorised the rhizobia into five species in the genera Rhizobium, Bradyrhizobium, Mesorhizobium and Agrobacterium. Land use system under tea had four of the five species found in the area whereas natural forests had two species. Land use significantly impacted on the diversity of rhizobia 2011 2014-09-24T07:58:04Z 2014-09-24T07:58:04Z Journal Article https://hdl.handle.net/10568/42494 en Open Access Mwenda, George, Nancy Karanja, Hamadi Boga, James Kahindi, A. Muigai, & D. Odee. "abundance and diversity of legume nodulating rhizobia in soils of Embu District, Kenya." Tropical and Subtropical Agroecosystems [Online], 13.1 (2011): 1-10. Web. 13 Jun. 2014 |
| spellingShingle | land use cropping systems feed legumes rhizobium symbiosis soil fertility utilización de la tierra sistemas de cultivo leguminosas forrajeras simbiosis fertilidad del suelo Mwenda, George M. Karanja, Nancy M. Boga, Hamadi M. Kahindi, James M. Muigai, A.M. Odee, D Abundance and diversity of legume nodulating rhizobia in soils of Embu district, Kenya |
| title | Abundance and diversity of legume nodulating rhizobia in soils of Embu district, Kenya |
| title_full | Abundance and diversity of legume nodulating rhizobia in soils of Embu district, Kenya |
| title_fullStr | Abundance and diversity of legume nodulating rhizobia in soils of Embu district, Kenya |
| title_full_unstemmed | Abundance and diversity of legume nodulating rhizobia in soils of Embu district, Kenya |
| title_short | Abundance and diversity of legume nodulating rhizobia in soils of Embu district, Kenya |
| title_sort | abundance and diversity of legume nodulating rhizobia in soils of embu district kenya |
| topic | land use cropping systems feed legumes rhizobium symbiosis soil fertility utilización de la tierra sistemas de cultivo leguminosas forrajeras simbiosis fertilidad del suelo |
| url | https://hdl.handle.net/10568/42494 |
| work_keys_str_mv | AT mwendageorgem abundanceanddiversityoflegumenodulatingrhizobiainsoilsofembudistrictkenya AT karanjanancym abundanceanddiversityoflegumenodulatingrhizobiainsoilsofembudistrictkenya AT bogahamadim abundanceanddiversityoflegumenodulatingrhizobiainsoilsofembudistrictkenya AT kahindijamesm abundanceanddiversityoflegumenodulatingrhizobiainsoilsofembudistrictkenya AT muigaiam abundanceanddiversityoflegumenodulatingrhizobiainsoilsofembudistrictkenya AT odeed abundanceanddiversityoflegumenodulatingrhizobiainsoilsofembudistrictkenya |