Analysis of polymorphisms in the merozoite surface protein-3a gene and two microsatellite loci in Sri Lankan Plasmodium vivax: evidence of population substructure in Sri Lanka
The geographical distribution of genetic variation in Plasmodium vivax samples ( N = 386) from nine districts across Sri Lanka is described using three markers; the P. vivax merozoite surface protein -3a ( Pvmsp -3a) gene, and the two microsatellites m1501 and m3502. At Pvmsp -3a, 11 alleles were fo...
| Main Authors: | , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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American Society of Tropical Medicine and Hygiene
2011
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| Online Access: | https://hdl.handle.net/10568/40459 |
| _version_ | 1855519468456771584 |
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| author | Schousboe, M.L. Rajakaruna, R.S. Amerasinghe, Priyanie H. Konradsen, Flemming Ord, R. Pearce, R. Bygbjerg, C. Roper, C. Alifrangis, M. |
| author_browse | Alifrangis, M. Amerasinghe, Priyanie H. Bygbjerg, C. Konradsen, Flemming Ord, R. Pearce, R. Rajakaruna, R.S. Roper, C. Schousboe, M.L. |
| author_facet | Schousboe, M.L. Rajakaruna, R.S. Amerasinghe, Priyanie H. Konradsen, Flemming Ord, R. Pearce, R. Bygbjerg, C. Roper, C. Alifrangis, M. |
| author_sort | Schousboe, M.L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The geographical distribution of genetic variation in Plasmodium vivax samples ( N = 386) from nine districts across Sri Lanka is described using three markers; the P. vivax merozoite surface protein -3a ( Pvmsp -3a) gene, and the two microsatellites m1501 and m3502. At Pvmsp -3a, 11 alleles were found with an expected heterozygosity (H e ) of 0.81, whereas at m1501 and m3502, 24 alleles (H e = 0.85) and 8 alleles (H e = 0.74) were detected, respectively. Overall, 95 unique three locus genotypes were detected among the 279 samples positive at all three loci (H e = 0.95). Calculating the pairwise fixation index ( F ST ) revealed statistically significant population structure. The presence of identical 2-loci microsatellite genotypes in a significant proportion of samples revealed local clusters of closely related isolates contributing to strong linkage disequilibrium between marker alleles. The results show evidence of high genetic diversity and possible population substructure of P. vivax populations in Sri Lanka. |
| format | Journal Article |
| id | CGSpace40459 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2011 |
| publishDateRange | 2011 |
| publishDateSort | 2011 |
| publisher | American Society of Tropical Medicine and Hygiene |
| publisherStr | American Society of Tropical Medicine and Hygiene |
| record_format | dspace |
| spelling | CGSpace404592025-06-17T08:23:26Z Analysis of polymorphisms in the merozoite surface protein-3a gene and two microsatellite loci in Sri Lankan Plasmodium vivax: evidence of population substructure in Sri Lanka Schousboe, M.L. Rajakaruna, R.S. Amerasinghe, Priyanie H. Konradsen, Flemming Ord, R. Pearce, R. Bygbjerg, C. Roper, C. Alifrangis, M. malaria plasmodium vivax population public health polymorphism satellite surveys statistical methods genetic variation virology infectious diseases parasitology The geographical distribution of genetic variation in Plasmodium vivax samples ( N = 386) from nine districts across Sri Lanka is described using three markers; the P. vivax merozoite surface protein -3a ( Pvmsp -3a) gene, and the two microsatellites m1501 and m3502. At Pvmsp -3a, 11 alleles were found with an expected heterozygosity (H e ) of 0.81, whereas at m1501 and m3502, 24 alleles (H e = 0.85) and 8 alleles (H e = 0.74) were detected, respectively. Overall, 95 unique three locus genotypes were detected among the 279 samples positive at all three loci (H e = 0.95). Calculating the pairwise fixation index ( F ST ) revealed statistically significant population structure. The presence of identical 2-loci microsatellite genotypes in a significant proportion of samples revealed local clusters of closely related isolates contributing to strong linkage disequilibrium between marker alleles. The results show evidence of high genetic diversity and possible population substructure of P. vivax populations in Sri Lanka. 2011-12-01 2014-06-13T14:47:43Z 2014-06-13T14:47:43Z Journal Article https://hdl.handle.net/10568/40459 en Limited Access American Society of Tropical Medicine and Hygiene Schousboe, M. L.; Rajakaruna, R. S.; Amerasinghe, Priyanie.; Konradsen, F.; Ord, R.; Pearce, R.; Bygbjerg, C.; Roper, C.; Alifrangis, M. 2011. Analysis of polymorphisms in the merozoite surface protein-3a gene and two microsatellite loci in Sri Lankan Plasmodium vivax: evidence of population substructure in Sri Lanka. American Journal of Tropical Medicine and Hygiene, 85(6):994-1001. doi: https://doi.org/10.4269/ajtmh.2011.11-0338 |
| spellingShingle | malaria plasmodium vivax population public health polymorphism satellite surveys statistical methods genetic variation virology infectious diseases parasitology Schousboe, M.L. Rajakaruna, R.S. Amerasinghe, Priyanie H. Konradsen, Flemming Ord, R. Pearce, R. Bygbjerg, C. Roper, C. Alifrangis, M. Analysis of polymorphisms in the merozoite surface protein-3a gene and two microsatellite loci in Sri Lankan Plasmodium vivax: evidence of population substructure in Sri Lanka |
| title | Analysis of polymorphisms in the merozoite surface protein-3a gene and two microsatellite loci in Sri Lankan Plasmodium vivax: evidence of population substructure in Sri Lanka |
| title_full | Analysis of polymorphisms in the merozoite surface protein-3a gene and two microsatellite loci in Sri Lankan Plasmodium vivax: evidence of population substructure in Sri Lanka |
| title_fullStr | Analysis of polymorphisms in the merozoite surface protein-3a gene and two microsatellite loci in Sri Lankan Plasmodium vivax: evidence of population substructure in Sri Lanka |
| title_full_unstemmed | Analysis of polymorphisms in the merozoite surface protein-3a gene and two microsatellite loci in Sri Lankan Plasmodium vivax: evidence of population substructure in Sri Lanka |
| title_short | Analysis of polymorphisms in the merozoite surface protein-3a gene and two microsatellite loci in Sri Lankan Plasmodium vivax: evidence of population substructure in Sri Lanka |
| title_sort | analysis of polymorphisms in the merozoite surface protein 3a gene and two microsatellite loci in sri lankan plasmodium vivax evidence of population substructure in sri lanka |
| topic | malaria plasmodium vivax population public health polymorphism satellite surveys statistical methods genetic variation virology infectious diseases parasitology |
| url | https://hdl.handle.net/10568/40459 |
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