Analysis of the immunoproteome of Mycoplasma mycoides subsp. mycoides small colony type reveals immunogenic homologues to other known virulence traits in related Mycoplasma species
Contagious bovine pleuropneumonia (CBPP) caused by Mycoplasma mycoides subsp. mycoides small colony type (MmmSC) has been eradicated in the developed world, but it is still present in many countries of sub-Saharan Africa. After initially successful control measures in the 1960s it has been spreading...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2009
|
| Online Access: | https://hdl.handle.net/10568/393 |
| _version_ | 1855515739732049920 |
|---|---|
| author | Jores, Joerg Meens, J. Buettner, F.F.R. Linz, B. Naessens, Jan Gerlach, G.F. |
| author_browse | Buettner, F.F.R. Gerlach, G.F. Jores, Joerg Linz, B. Meens, J. Naessens, Jan |
| author_facet | Jores, Joerg Meens, J. Buettner, F.F.R. Linz, B. Naessens, Jan Gerlach, G.F. |
| author_sort | Jores, Joerg |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Contagious bovine pleuropneumonia (CBPP) caused by Mycoplasma mycoides subsp. mycoides small colony type (MmmSC) has been eradicated in the developed world, but it is still present in many countries of sub-Saharan Africa. After initially successful control measures in the 1960s it has been spreading due to a lack of money, fragmentation of veterinary services, uncontrolled cattle movement, insufficient vaccine efficacy and sensitivity of current diagnostic tests. In this study we used two-dimensional polyacrylamide gel electrophoresis followed by immunoblot with sera from MmmSC-infected animals and MALDI-ToF mass spectrometry to identify novel immunogenic proteins as candidate molecules for improved diagnostics and vaccines. We identified 24 immunogens recognized by pooled sera from experimentally infected cattle. Furthermore, a serum from an animal with acute clinical disease as well as severe pathomorphological lesions recognized 13 additional immunogens indicating variation in the antibody responses to CBPP amongst cattle. Most immunogens showed compelling similarity to protein/gene sequences in the two ruminant pathogens M. capricolum subsp. capricolum and M. mycoides subsp. mycoides large colony type both belonging to the mycoides cluster. Three of these proteins, namely glycerol-3-phosphate oxidase, adenylosuccinate synthase, and glyceraldehyde-3-phosphate dehydrogenase, had no compelling homologue in the other distantly related bovine pathogen M. agalactiae. In addition, translation elongation factor Tu, heat shock protein 70, pyruvate dehydrogenase, and FKBP-type peptidyl-prolyl isomerase, which have been found to mediate adhesion to host tissue in other mycoplasmas were shown to be expressed and recognized by sera. These proteins have potential for the development of improved diagnostic tests and possibly vaccines. |
| format | Journal Article |
| id | CGSpace393 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace3932023-12-08T19:36:04Z Analysis of the immunoproteome of Mycoplasma mycoides subsp. mycoides small colony type reveals immunogenic homologues to other known virulence traits in related Mycoplasma species Jores, Joerg Meens, J. Buettner, F.F.R. Linz, B. Naessens, Jan Gerlach, G.F. Contagious bovine pleuropneumonia (CBPP) caused by Mycoplasma mycoides subsp. mycoides small colony type (MmmSC) has been eradicated in the developed world, but it is still present in many countries of sub-Saharan Africa. After initially successful control measures in the 1960s it has been spreading due to a lack of money, fragmentation of veterinary services, uncontrolled cattle movement, insufficient vaccine efficacy and sensitivity of current diagnostic tests. In this study we used two-dimensional polyacrylamide gel electrophoresis followed by immunoblot with sera from MmmSC-infected animals and MALDI-ToF mass spectrometry to identify novel immunogenic proteins as candidate molecules for improved diagnostics and vaccines. We identified 24 immunogens recognized by pooled sera from experimentally infected cattle. Furthermore, a serum from an animal with acute clinical disease as well as severe pathomorphological lesions recognized 13 additional immunogens indicating variation in the antibody responses to CBPP amongst cattle. Most immunogens showed compelling similarity to protein/gene sequences in the two ruminant pathogens M. capricolum subsp. capricolum and M. mycoides subsp. mycoides large colony type both belonging to the mycoides cluster. Three of these proteins, namely glycerol-3-phosphate oxidase, adenylosuccinate synthase, and glyceraldehyde-3-phosphate dehydrogenase, had no compelling homologue in the other distantly related bovine pathogen M. agalactiae. In addition, translation elongation factor Tu, heat shock protein 70, pyruvate dehydrogenase, and FKBP-type peptidyl-prolyl isomerase, which have been found to mediate adhesion to host tissue in other mycoplasmas were shown to be expressed and recognized by sera. These proteins have potential for the development of improved diagnostic tests and possibly vaccines. 2009-10 2010-01-13T10:54:33Z 2010-01-13T10:54:33Z Journal Article https://hdl.handle.net/10568/393 en Limited Access Elsevier Jores, J.; Meens, J.; Buettner, F.F.R.; Linz, B.; Naessens, J.; Gerlach, G.F. 2009. Analysis of the immunoproteome of Mycoplasma mycoides subsp. mycoides small colony type reveals immunogenic homologues to other known virulence traits in related Mycoplasma species. Veterinary Immunology and Immunopathology. v. 131(3-4). p. 238-245. |
| spellingShingle | Jores, Joerg Meens, J. Buettner, F.F.R. Linz, B. Naessens, Jan Gerlach, G.F. Analysis of the immunoproteome of Mycoplasma mycoides subsp. mycoides small colony type reveals immunogenic homologues to other known virulence traits in related Mycoplasma species |
| title | Analysis of the immunoproteome of Mycoplasma mycoides subsp. mycoides small colony type reveals immunogenic homologues to other known virulence traits in related Mycoplasma species |
| title_full | Analysis of the immunoproteome of Mycoplasma mycoides subsp. mycoides small colony type reveals immunogenic homologues to other known virulence traits in related Mycoplasma species |
| title_fullStr | Analysis of the immunoproteome of Mycoplasma mycoides subsp. mycoides small colony type reveals immunogenic homologues to other known virulence traits in related Mycoplasma species |
| title_full_unstemmed | Analysis of the immunoproteome of Mycoplasma mycoides subsp. mycoides small colony type reveals immunogenic homologues to other known virulence traits in related Mycoplasma species |
| title_short | Analysis of the immunoproteome of Mycoplasma mycoides subsp. mycoides small colony type reveals immunogenic homologues to other known virulence traits in related Mycoplasma species |
| title_sort | analysis of the immunoproteome of mycoplasma mycoides subsp mycoides small colony type reveals immunogenic homologues to other known virulence traits in related mycoplasma species |
| url | https://hdl.handle.net/10568/393 |
| work_keys_str_mv | AT joresjoerg analysisoftheimmunoproteomeofmycoplasmamycoidessubspmycoidessmallcolonytyperevealsimmunogenichomologuestootherknownvirulencetraitsinrelatedmycoplasmaspecies AT meensj analysisoftheimmunoproteomeofmycoplasmamycoidessubspmycoidessmallcolonytyperevealsimmunogenichomologuestootherknownvirulencetraitsinrelatedmycoplasmaspecies AT buettnerffr analysisoftheimmunoproteomeofmycoplasmamycoidessubspmycoidessmallcolonytyperevealsimmunogenichomologuestootherknownvirulencetraitsinrelatedmycoplasmaspecies AT linzb analysisoftheimmunoproteomeofmycoplasmamycoidessubspmycoidessmallcolonytyperevealsimmunogenichomologuestootherknownvirulencetraitsinrelatedmycoplasmaspecies AT naessensjan analysisoftheimmunoproteomeofmycoplasmamycoidessubspmycoidessmallcolonytyperevealsimmunogenichomologuestootherknownvirulencetraitsinrelatedmycoplasmaspecies AT gerlachgf analysisoftheimmunoproteomeofmycoplasmamycoidessubspmycoidessmallcolonytyperevealsimmunogenichomologuestootherknownvirulencetraitsinrelatedmycoplasmaspecies |