Expression of a rice chitinase gene in transgenic banana (''Gros Michel'', AAA genome group) confers resistance to black leaf streak disease
Transgenic banana (Musa acuminata 'Gros Michel') integrating either of two rice chitinase genes was generated and its resistance to Black Leaf Streak disease caused by the fungus Mycosphaerella fijiensis was tested using a leaf disk bioassay. PCR screening indicated the presence of the hpt selectabl...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2013
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/35582 |
| _version_ | 1855542778797228032 |
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| author | Kovacs, G. Sagi, L. Jacon, G. Arinaitwe, G. Busogoro, J.P. Thiry, E. Strosse, H. Swennen, Rony L. Remy, S. |
| author_browse | Arinaitwe, G. Busogoro, J.P. Jacon, G. Kovacs, G. Remy, S. Sagi, L. Strosse, H. Swennen, Rony L. Thiry, E. |
| author_facet | Kovacs, G. Sagi, L. Jacon, G. Arinaitwe, G. Busogoro, J.P. Thiry, E. Strosse, H. Swennen, Rony L. Remy, S. |
| author_sort | Kovacs, G. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Transgenic banana (Musa acuminata 'Gros Michel') integrating either of two rice chitinase genes was generated and its resistance to Black Leaf Streak disease caused by the fungus Mycosphaerella fijiensis was tested using a leaf disk bioassay. PCR screening indicated the presence of the hpt selectable marker gene in more than 90 % of the lines tested, whereas more than three quarters of the lines contained the linked rice chitinase gene resulting in a co-transformation frequency of at least 71.4 %. Further, a unique stable integration of the transgenes in each line revealed some false negative PCR results and the expected co-transformation frequency of 100 %. |
| format | Journal Article |
| id | CGSpace35582 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace355822025-11-12T05:49:46Z Expression of a rice chitinase gene in transgenic banana (''Gros Michel'', AAA genome group) confers resistance to black leaf streak disease Kovacs, G. Sagi, L. Jacon, G. Arinaitwe, G. Busogoro, J.P. Thiry, E. Strosse, H. Swennen, Rony L. Remy, S. musa (bananas) mycosphaerella fijiensis plant diseases transgenic plants transgenics Transgenic banana (Musa acuminata 'Gros Michel') integrating either of two rice chitinase genes was generated and its resistance to Black Leaf Streak disease caused by the fungus Mycosphaerella fijiensis was tested using a leaf disk bioassay. PCR screening indicated the presence of the hpt selectable marker gene in more than 90 % of the lines tested, whereas more than three quarters of the lines contained the linked rice chitinase gene resulting in a co-transformation frequency of at least 71.4 %. Further, a unique stable integration of the transgenes in each line revealed some false negative PCR results and the expected co-transformation frequency of 100 %. 2013-02 2014-05-05T08:44:27Z 2014-05-05T08:44:27Z Journal Article https://hdl.handle.net/10568/35582 en Open Access application/pdf Springer Kovacs, G.; Sagi, L.; Jacon, G.; Arinaitwe, G.; Busogoro, J.P.; Thiry, E.; Strosse, H.; Swennen, R.; Remy, S. -2013-Expression of a rice chitinase gene in transgenic banana (''Gros Michel'', AAA genome group) confers resistance to black leaf streak disease-Transgenic Research 22(1)-p. 117-130 |
| spellingShingle | musa (bananas) mycosphaerella fijiensis plant diseases transgenic plants transgenics Kovacs, G. Sagi, L. Jacon, G. Arinaitwe, G. Busogoro, J.P. Thiry, E. Strosse, H. Swennen, Rony L. Remy, S. Expression of a rice chitinase gene in transgenic banana (''Gros Michel'', AAA genome group) confers resistance to black leaf streak disease |
| title | Expression of a rice chitinase gene in transgenic banana (''Gros Michel'', AAA genome group) confers resistance to black leaf streak disease |
| title_full | Expression of a rice chitinase gene in transgenic banana (''Gros Michel'', AAA genome group) confers resistance to black leaf streak disease |
| title_fullStr | Expression of a rice chitinase gene in transgenic banana (''Gros Michel'', AAA genome group) confers resistance to black leaf streak disease |
| title_full_unstemmed | Expression of a rice chitinase gene in transgenic banana (''Gros Michel'', AAA genome group) confers resistance to black leaf streak disease |
| title_short | Expression of a rice chitinase gene in transgenic banana (''Gros Michel'', AAA genome group) confers resistance to black leaf streak disease |
| title_sort | expression of a rice chitinase gene in transgenic banana gros michel aaa genome group confers resistance to black leaf streak disease |
| topic | musa (bananas) mycosphaerella fijiensis plant diseases transgenic plants transgenics |
| url | https://hdl.handle.net/10568/35582 |
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