Massive sorghum collection genotyped with SSR markers to enhance use of global genetic resources

Large ex situ collections require approaches for sampling manageable amounts of germplasm for in-depth characterization and use. We present here a large diversity survey in sorghum with 3367 accessions and 41 reference nuclear SSR markers. Of 19 alleles on average per locus, the largest numbers of a...

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Autores principales: Billot, C., Ramu, P., Bouchet, S., Chantereau, J., Deu, M., Gardes, L., Noyer, J., Rami, J.F., Rivallan, R., Li, Y., Lu, P., Wang, T., Folkertsma, R.T., Arnaud, Elizabeth, Upadhyaya, Hari D., Glaszmann, Jean-Christophe, Hash, C.T.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://hdl.handle.net/10568/35541
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author Billot, C.
Ramu, P.
Bouchet, S.
Chantereau, J.
Deu, M.
Gardes, L.
Noyer, J.
Rami, J.F.
Rivallan, R.
Li, Y.
Lu, P.
Wang, T.
Folkertsma, R.T.
Arnaud, Elizabeth
Upadhyaya, Hari D.
Glaszmann, Jean-Christophe
Hash, C.T.
author_browse Arnaud, Elizabeth
Billot, C.
Bouchet, S.
Chantereau, J.
Deu, M.
Folkertsma, R.T.
Gardes, L.
Glaszmann, Jean-Christophe
Hash, C.T.
Li, Y.
Lu, P.
Noyer, J.
Rami, J.F.
Ramu, P.
Rivallan, R.
Upadhyaya, Hari D.
Wang, T.
author_facet Billot, C.
Ramu, P.
Bouchet, S.
Chantereau, J.
Deu, M.
Gardes, L.
Noyer, J.
Rami, J.F.
Rivallan, R.
Li, Y.
Lu, P.
Wang, T.
Folkertsma, R.T.
Arnaud, Elizabeth
Upadhyaya, Hari D.
Glaszmann, Jean-Christophe
Hash, C.T.
author_sort Billot, C.
collection Repository of Agricultural Research Outputs (CGSpace)
description Large ex situ collections require approaches for sampling manageable amounts of germplasm for in-depth characterization and use. We present here a large diversity survey in sorghum with 3367 accessions and 41 reference nuclear SSR markers. Of 19 alleles on average per locus, the largest numbers of alleles were concentrated in central and eastern Africa. Cultivated sorghum appeared structured according to geographic regions and race within region. A total of 13 groups of variable size were distinguished. The peripheral groups in western Africa, southern Africa and eastern Asia were the most homogeneous and clearly differentiated. Except for Kafir, there was little correspondence between races and marker-based groups. Bicolor, Caudatum, Durra and Guinea types were each dispersed in three groups or more. Races should therefore better be referred to as morphotypes. Wild and weedy accessions were very diverse and scattered among cultivated samples, reinforcing the idea that large gene-flow exists between the different compartments. Our study provides an entry to global sorghum germplasm collections. Our reference marker kit can serve to aggregate additional studies and enhance international collaboration. We propose a core reference set in order to facilitate integrated phenotyping experiments towards refined functional understanding of sorghum diversity.
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spelling CGSpace355412025-11-12T05:44:21Z Massive sorghum collection genotyped with SSR markers to enhance use of global genetic resources Billot, C. Ramu, P. Bouchet, S. Chantereau, J. Deu, M. Gardes, L. Noyer, J. Rami, J.F. Rivallan, R. Li, Y. Lu, P. Wang, T. Folkertsma, R.T. Arnaud, Elizabeth Upadhyaya, Hari D. Glaszmann, Jean-Christophe Hash, C.T. documentation gene banks genetic markers genetic resources germplasm collections sorghum Large ex situ collections require approaches for sampling manageable amounts of germplasm for in-depth characterization and use. We present here a large diversity survey in sorghum with 3367 accessions and 41 reference nuclear SSR markers. Of 19 alleles on average per locus, the largest numbers of alleles were concentrated in central and eastern Africa. Cultivated sorghum appeared structured according to geographic regions and race within region. A total of 13 groups of variable size were distinguished. The peripheral groups in western Africa, southern Africa and eastern Asia were the most homogeneous and clearly differentiated. Except for Kafir, there was little correspondence between races and marker-based groups. Bicolor, Caudatum, Durra and Guinea types were each dispersed in three groups or more. Races should therefore better be referred to as morphotypes. Wild and weedy accessions were very diverse and scattered among cultivated samples, reinforcing the idea that large gene-flow exists between the different compartments. Our study provides an entry to global sorghum germplasm collections. Our reference marker kit can serve to aggregate additional studies and enhance international collaboration. We propose a core reference set in order to facilitate integrated phenotyping experiments towards refined functional understanding of sorghum diversity. 2013 2014-05-05T08:44:20Z 2014-05-05T08:44:20Z Journal Article https://hdl.handle.net/10568/35541 en Open Access application/pdf Public Library of Science Billot, C.; Ramu, P.; Bouchet, S.; Chantereau, J.; Deu, M.; Gardes, L.; Noyer, J.; Rami, J.; Rivallan, R.; Li, Y.; Lu, P.; Wang, T.; Folkertsma, R.T.; Arnaud, E.; Upadhyaya, H.D.; Glaszmann, J.C.; Hash, C.T. -2013-Massive sorghum collection genotyped with SSR markers to enhance use of global genetic resources-PLoS ONE 8(4) -e59714
spellingShingle documentation
gene banks
genetic markers
genetic resources
germplasm collections
sorghum
Billot, C.
Ramu, P.
Bouchet, S.
Chantereau, J.
Deu, M.
Gardes, L.
Noyer, J.
Rami, J.F.
Rivallan, R.
Li, Y.
Lu, P.
Wang, T.
Folkertsma, R.T.
Arnaud, Elizabeth
Upadhyaya, Hari D.
Glaszmann, Jean-Christophe
Hash, C.T.
Massive sorghum collection genotyped with SSR markers to enhance use of global genetic resources
title Massive sorghum collection genotyped with SSR markers to enhance use of global genetic resources
title_full Massive sorghum collection genotyped with SSR markers to enhance use of global genetic resources
title_fullStr Massive sorghum collection genotyped with SSR markers to enhance use of global genetic resources
title_full_unstemmed Massive sorghum collection genotyped with SSR markers to enhance use of global genetic resources
title_short Massive sorghum collection genotyped with SSR markers to enhance use of global genetic resources
title_sort massive sorghum collection genotyped with ssr markers to enhance use of global genetic resources
topic documentation
gene banks
genetic markers
genetic resources
germplasm collections
sorghum
url https://hdl.handle.net/10568/35541
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