Some pharmacokinetic parameters of the trypanocidal drug homidium bromide in Friesian and Boran steers using an enzyme-linked immunosorbent assay (ELISA)
Pharmacokinetic studies on the trypanocidal drug homidium bromide using a competitive enzyme immunoassay (detection limit 0.1 ng/mL) are reported for non-infected Friesian and Boran steers following treatment with homidium bromide at a dose of 1.0 mg/kg b.w. Following intravenous (i.v.) treatment of...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
1999
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| Online Access: | https://hdl.handle.net/10568/35309 |
| _version_ | 1855527973004771328 |
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| author | Murilla, G. Holmes, P.H. Peregrine, A.S. Eisler, M.C. Ndung'u, J.M. |
| author_browse | Eisler, M.C. Holmes, P.H. Murilla, G. Ndung'u, J.M. Peregrine, A.S. |
| author_facet | Murilla, G. Holmes, P.H. Peregrine, A.S. Eisler, M.C. Ndung'u, J.M. |
| author_sort | Murilla, G. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Pharmacokinetic studies on the trypanocidal drug homidium bromide using a competitive enzyme immunoassay (detection limit 0.1 ng/mL) are reported for non-infected Friesian and Boran steers following treatment with homidium bromide at a dose of 1.0 mg/kg b.w. Following intravenous (i.v.) treatment of Friesian steers (n = 5), the mean serum drug concentrations were 31.9 ± 2.1 and 3.9 ± 0.4 ng/ml at 1 and 24 h, respectively. The decline in serum drug concentration was tri-exponential with half-lives of 0.064 ± 0.037 h for t( 1/2 ?), 7.17 ± 1.87 h for T( 1/2 ?) and 106.3 ± 6.6 h for t( 1/2 ?) for distribution and elimination phases 1 and 2, respectively. Drug was detectable in serum for 17 days following treatment. The mean residence time (MRT) was 63.4 ± 7.5 h. Following intramuscular (i.m.) treatment of Friesian steers (n = 5), the drug concentration at 1 h after treatment was 72.5 ± 2.2 ng/mL. This declined to 9.8 ± 1.8 ng/mL at 24 h. Low concentrations of between 0.1 and 0.3 ng/mL remained in circulation for up to 90 days post-treatment. Following intramuscular treatment of Boran steers (n = 5), the mean serum drug concentration at 1 h after treatment was 112.1 ± 40.3 ng/mL. By 24 h after treatment, the concentration had fallen to 13.0 ± 3.3 ng/mL. Thereafter, the serum drug concentration-versus-time profile and the pharmacokinetic parameters obtained following non-compartmental analysis were similar to those obtained following intramuscular treatment of Friesian steers. |
| format | Journal Article |
| id | CGSpace35309 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1999 |
| publishDateRange | 1999 |
| publishDateSort | 1999 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace353092024-08-27T12:27:43Z Some pharmacokinetic parameters of the trypanocidal drug homidium bromide in Friesian and Boran steers using an enzyme-linked immunosorbent assay (ELISA) Murilla, G. Holmes, P.H. Peregrine, A.S. Eisler, M.C. Ndung'u, J.M. vaccination cattle Pharmacokinetic studies on the trypanocidal drug homidium bromide using a competitive enzyme immunoassay (detection limit 0.1 ng/mL) are reported for non-infected Friesian and Boran steers following treatment with homidium bromide at a dose of 1.0 mg/kg b.w. Following intravenous (i.v.) treatment of Friesian steers (n = 5), the mean serum drug concentrations were 31.9 ± 2.1 and 3.9 ± 0.4 ng/ml at 1 and 24 h, respectively. The decline in serum drug concentration was tri-exponential with half-lives of 0.064 ± 0.037 h for t( 1/2 ?), 7.17 ± 1.87 h for T( 1/2 ?) and 106.3 ± 6.6 h for t( 1/2 ?) for distribution and elimination phases 1 and 2, respectively. Drug was detectable in serum for 17 days following treatment. The mean residence time (MRT) was 63.4 ± 7.5 h. Following intramuscular (i.m.) treatment of Friesian steers (n = 5), the drug concentration at 1 h after treatment was 72.5 ± 2.2 ng/mL. This declined to 9.8 ± 1.8 ng/mL at 24 h. Low concentrations of between 0.1 and 0.3 ng/mL remained in circulation for up to 90 days post-treatment. Following intramuscular treatment of Boran steers (n = 5), the mean serum drug concentration at 1 h after treatment was 112.1 ± 40.3 ng/mL. By 24 h after treatment, the concentration had fallen to 13.0 ± 3.3 ng/mL. Thereafter, the serum drug concentration-versus-time profile and the pharmacokinetic parameters obtained following non-compartmental analysis were similar to those obtained following intramuscular treatment of Friesian steers. 1999-10 2014-04-14T10:55:54Z 2014-04-14T10:55:54Z Journal Article https://hdl.handle.net/10568/35309 en Limited Access Wiley Murilla, G.A., Holmes, P.H., Peregrine, A.S., Eisler, M.C. and Ndung'u, J.M. 1999. Some pharmacokinetic parameters of the trypanocidal drug homidium bromide in Friesian and Boran steers using an enzyme-linked immunosorbent assay (ELISA). Journal of Veterinary Pharmacology and Therapeutics 22(5): 295-300. |
| spellingShingle | vaccination cattle Murilla, G. Holmes, P.H. Peregrine, A.S. Eisler, M.C. Ndung'u, J.M. Some pharmacokinetic parameters of the trypanocidal drug homidium bromide in Friesian and Boran steers using an enzyme-linked immunosorbent assay (ELISA) |
| title | Some pharmacokinetic parameters of the trypanocidal drug homidium bromide in Friesian and Boran steers using an enzyme-linked immunosorbent assay (ELISA) |
| title_full | Some pharmacokinetic parameters of the trypanocidal drug homidium bromide in Friesian and Boran steers using an enzyme-linked immunosorbent assay (ELISA) |
| title_fullStr | Some pharmacokinetic parameters of the trypanocidal drug homidium bromide in Friesian and Boran steers using an enzyme-linked immunosorbent assay (ELISA) |
| title_full_unstemmed | Some pharmacokinetic parameters of the trypanocidal drug homidium bromide in Friesian and Boran steers using an enzyme-linked immunosorbent assay (ELISA) |
| title_short | Some pharmacokinetic parameters of the trypanocidal drug homidium bromide in Friesian and Boran steers using an enzyme-linked immunosorbent assay (ELISA) |
| title_sort | some pharmacokinetic parameters of the trypanocidal drug homidium bromide in friesian and boran steers using an enzyme linked immunosorbent assay elisa |
| topic | vaccination cattle |
| url | https://hdl.handle.net/10568/35309 |
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