Gut transcriptome of replete adult female cattle ticks, Rhipicephalus (Boophilus) microplus, feeding upon a Babesia bovis-infected bovine host
As it feeds upon cattle, Rhipicephalus (Boophilus) microplus is capable of transmitting a number of pathogenic organisms, including the apicomplexan hemoparasite Babesia bovis, a causative agent of bovine babesiosis. The R. microplus female gut transcriptome was studied for two cohorts: adult female...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2013
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/35307 |
| _version_ | 1855516009958473728 |
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| author | Heekin, A.M. Guerrero, F.D. Bendele, K.G. Saldivar, L. Scoles, Glen A. Dowd, S.E. Gondro, C. Nene, Vishvanath M. Djikeng, Appolinaire Brayton, K.A. |
| author_browse | Bendele, K.G. Brayton, K.A. Djikeng, Appolinaire Dowd, S.E. Gondro, C. Guerrero, F.D. Heekin, A.M. Nene, Vishvanath M. Saldivar, L. Scoles, Glen A. |
| author_facet | Heekin, A.M. Guerrero, F.D. Bendele, K.G. Saldivar, L. Scoles, Glen A. Dowd, S.E. Gondro, C. Nene, Vishvanath M. Djikeng, Appolinaire Brayton, K.A. |
| author_sort | Heekin, A.M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | As it feeds upon cattle, Rhipicephalus (Boophilus) microplus is capable of transmitting a number of pathogenic organisms, including the apicomplexan hemoparasite Babesia bovis, a causative agent of bovine babesiosis. The R. microplus female gut transcriptome was studied for two cohorts: adult females feeding on a bovine host infected with B. bovis and adult females feeding on an uninfected bovine. RNA was purified and used to generate a subtracted cDNA library from B. bovis-infected female gut, and 4,077 expressed sequence tags (ESTs) were sequenced. Gene expression was also measured by a microarray designed from the publicly available R. microplus gene index: BmiGI Version 2. We compared gene expression in the tick gut from females feeding upon an uninfected bovine to gene expression in tick gut from females feeding upon a splenectomized bovine infected with B. bovis. Thirty-three ESTs represented on the microarray were expressed at a higher level in female gut samples from the ticks feeding upon a B. bovis-infected calf compared to expression levels in female gut samples from ticks feeding on an uninfected calf. Forty-three transcripts were expressed at a lower level in the ticks feeding upon B. bovis-infected female guts compared with expression in female gut samples from ticks feeding on the uninfected calf. These array data were used as initial characterization of gene expression associated with the infection of R. microplus by B. bovis. |
| format | Journal Article |
| id | CGSpace35307 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace353072024-09-10T08:32:51Z Gut transcriptome of replete adult female cattle ticks, Rhipicephalus (Boophilus) microplus, feeding upon a Babesia bovis-infected bovine host Heekin, A.M. Guerrero, F.D. Bendele, K.G. Saldivar, L. Scoles, Glen A. Dowd, S.E. Gondro, C. Nene, Vishvanath M. Djikeng, Appolinaire Brayton, K.A. cattle animal diseases infectious diseases parasitology As it feeds upon cattle, Rhipicephalus (Boophilus) microplus is capable of transmitting a number of pathogenic organisms, including the apicomplexan hemoparasite Babesia bovis, a causative agent of bovine babesiosis. The R. microplus female gut transcriptome was studied for two cohorts: adult females feeding on a bovine host infected with B. bovis and adult females feeding on an uninfected bovine. RNA was purified and used to generate a subtracted cDNA library from B. bovis-infected female gut, and 4,077 expressed sequence tags (ESTs) were sequenced. Gene expression was also measured by a microarray designed from the publicly available R. microplus gene index: BmiGI Version 2. We compared gene expression in the tick gut from females feeding upon an uninfected bovine to gene expression in tick gut from females feeding upon a splenectomized bovine infected with B. bovis. Thirty-three ESTs represented on the microarray were expressed at a higher level in female gut samples from the ticks feeding upon a B. bovis-infected calf compared to expression levels in female gut samples from ticks feeding on an uninfected calf. Forty-three transcripts were expressed at a lower level in the ticks feeding upon B. bovis-infected female guts compared with expression in female gut samples from ticks feeding on the uninfected calf. These array data were used as initial characterization of gene expression associated with the infection of R. microplus by B. bovis. 2013-09 2014-04-14T10:55:54Z 2014-04-14T10:55:54Z Journal Article https://hdl.handle.net/10568/35307 en Limited Access Springer Heekin, A.M., Guerrero, F.D., Bendele, K.G., Saldivar, L., Scoles, G.A., Dowd, S.E., Gondro, C., Nene, V., Djikeng, A. Brayton, K.A. 2013. Gut transcriptome of replete adult female cattle ticks, Rhipicephalus (Boophilus) microplus, feeding upon a Babesia bovis-infected bovine host. Parasitology Research 112(9):3075-3090.. |
| spellingShingle | cattle animal diseases infectious diseases parasitology Heekin, A.M. Guerrero, F.D. Bendele, K.G. Saldivar, L. Scoles, Glen A. Dowd, S.E. Gondro, C. Nene, Vishvanath M. Djikeng, Appolinaire Brayton, K.A. Gut transcriptome of replete adult female cattle ticks, Rhipicephalus (Boophilus) microplus, feeding upon a Babesia bovis-infected bovine host |
| title | Gut transcriptome of replete adult female cattle ticks, Rhipicephalus (Boophilus) microplus, feeding upon a Babesia bovis-infected bovine host |
| title_full | Gut transcriptome of replete adult female cattle ticks, Rhipicephalus (Boophilus) microplus, feeding upon a Babesia bovis-infected bovine host |
| title_fullStr | Gut transcriptome of replete adult female cattle ticks, Rhipicephalus (Boophilus) microplus, feeding upon a Babesia bovis-infected bovine host |
| title_full_unstemmed | Gut transcriptome of replete adult female cattle ticks, Rhipicephalus (Boophilus) microplus, feeding upon a Babesia bovis-infected bovine host |
| title_short | Gut transcriptome of replete adult female cattle ticks, Rhipicephalus (Boophilus) microplus, feeding upon a Babesia bovis-infected bovine host |
| title_sort | gut transcriptome of replete adult female cattle ticks rhipicephalus boophilus microplus feeding upon a babesia bovis infected bovine host |
| topic | cattle animal diseases infectious diseases parasitology |
| url | https://hdl.handle.net/10568/35307 |
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