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Specific PCR assay for a tannin-tolerant Selenomonas ruminantium isolate, derived from helicase coding sequences

Sequences from a tannin-tolerant Selenomonas ruminantium isolate (EAT2) that hydrolyzes gallic acid were identified. Two exhibited identity to helicases with a wide phylogenetic distribution. PCR amplification by using primers from one helicase gene detected 2,000 to 5,000 EAT2 genome equivalents bu...

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Main Authors: Bishop, Richard P., Obura, M., Odongo, D., Odenyo, A.A.
Format: Journal Article
Language:Inglés
Published: American Society for Microbiology 2004
Subjects:
animal diseases
genetics
ecology
food science
biotechnology
Online Access:https://hdl.handle.net/10568/35306
Summary:Sequences from a tannin-tolerant Selenomonas ruminantium isolate (EAT2) that hydrolyzes gallic acid were identified. Two exhibited identity to helicases with a wide phylogenetic distribution. PCR amplification by using primers from one helicase gene detected 2,000 to 5,000 EAT2 genome equivalents but did not amplify total gastrointestinal microbial DNA of nine other ungulate species.

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