Comparison of SSR and AFLP analysis for genetic diversity assessment of Ethiopian arabica coffee genotypes
Knowledge of genetic diversity within and among genotypes of any crop is fundamental for estimation of the potential genetic gain in a breeding programme and effective conservation of available genetic resources. Currently, different molecular marker techniques are being developed for measuring gene...
| Autores principales: | , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Informa UK Limited
2009
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/35275 |
| _version_ | 1855542525394157568 |
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| author | Dessalegn, Yigsaw Herselman, L. Labuschagne, Maryke T. |
| author_browse | Dessalegn, Yigsaw Herselman, L. Labuschagne, Maryke T. |
| author_facet | Dessalegn, Yigsaw Herselman, L. Labuschagne, Maryke T. |
| author_sort | Dessalegn, Yigsaw |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Knowledge of genetic diversity within and among genotypes of any crop is fundamental for estimation of the potential genetic gain in a breeding programme and effective conservation of available genetic resources. Currently, different molecular marker techniques are being developed for measuring genetic diversity. Comparison among molecular marker techniques is important for effective marker selection. However such types of efforts are rare for arabica coffee. This study was conducted to compare the efficiency of simple sequence repeat (SSR) analysis for determining genetic relationships of 28 Coffea arabica L genotypes collected from different parts of Ethiopia with work previously done using amplified fragment length polymorphism (AFLP) analysis. A total of 22 SSR fragments were amplified and compared with 712 previously amplified AFLP fragments. AFLP and SSR markers were positively and significantly correlated (0.217) in estimating genetic similarity among genotypes. The average genetic similarity coefficient calculated using SSR markers was much lower (0.560 with a range of 0.286-1.000) compared to AFLP markers (0.915 with a range of 0.860-0.982) indicating the higher information content of SSR markers. AFLP markers distinguished all genotypes, while SSR markers distinguished 64.3% of the genotypes. Results indicated that AFLP markers were more efficient compared to SSR markers for characterization of the evaluated coffee genotypes. |
| format | Journal Article |
| id | CGSpace35275 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | Informa UK Limited |
| publisherStr | Informa UK Limited |
| record_format | dspace |
| spelling | CGSpace352752024-08-27T12:27:43Z Comparison of SSR and AFLP analysis for genetic diversity assessment of Ethiopian arabica coffee genotypes Dessalegn, Yigsaw Herselman, L. Labuschagne, Maryke T. research crops Knowledge of genetic diversity within and among genotypes of any crop is fundamental for estimation of the potential genetic gain in a breeding programme and effective conservation of available genetic resources. Currently, different molecular marker techniques are being developed for measuring genetic diversity. Comparison among molecular marker techniques is important for effective marker selection. However such types of efforts are rare for arabica coffee. This study was conducted to compare the efficiency of simple sequence repeat (SSR) analysis for determining genetic relationships of 28 Coffea arabica L genotypes collected from different parts of Ethiopia with work previously done using amplified fragment length polymorphism (AFLP) analysis. A total of 22 SSR fragments were amplified and compared with 712 previously amplified AFLP fragments. AFLP and SSR markers were positively and significantly correlated (0.217) in estimating genetic similarity among genotypes. The average genetic similarity coefficient calculated using SSR markers was much lower (0.560 with a range of 0.286-1.000) compared to AFLP markers (0.915 with a range of 0.860-0.982) indicating the higher information content of SSR markers. AFLP markers distinguished all genotypes, while SSR markers distinguished 64.3% of the genotypes. Results indicated that AFLP markers were more efficient compared to SSR markers for characterization of the evaluated coffee genotypes. 2009-01 2014-04-14T10:55:45Z 2014-04-14T10:55:45Z Journal Article https://hdl.handle.net/10568/35275 en Open Access Informa UK Limited Dessalegn, Y., Herselman, L., Labuschagne, M. 2009. Comparison of SSR and AFLP analysis for genetic diversity assessment of Ethiopian arabica coffee genotypes. South African Journal of Plant and Soil 26(2): 119 - 125 |
| spellingShingle | research crops Dessalegn, Yigsaw Herselman, L. Labuschagne, Maryke T. Comparison of SSR and AFLP analysis for genetic diversity assessment of Ethiopian arabica coffee genotypes |
| title | Comparison of SSR and AFLP analysis for genetic diversity assessment of Ethiopian arabica coffee genotypes |
| title_full | Comparison of SSR and AFLP analysis for genetic diversity assessment of Ethiopian arabica coffee genotypes |
| title_fullStr | Comparison of SSR and AFLP analysis for genetic diversity assessment of Ethiopian arabica coffee genotypes |
| title_full_unstemmed | Comparison of SSR and AFLP analysis for genetic diversity assessment of Ethiopian arabica coffee genotypes |
| title_short | Comparison of SSR and AFLP analysis for genetic diversity assessment of Ethiopian arabica coffee genotypes |
| title_sort | comparison of ssr and aflp analysis for genetic diversity assessment of ethiopian arabica coffee genotypes |
| topic | research crops |
| url | https://hdl.handle.net/10568/35275 |
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