Homologous minicircles in Leishmania donovani
Leishmania minicircular deoxyribonucleic acid (DNA) is arranged into different classes according to sequence. These classes differ substantially in sequence, despite species- and genus-specific regions, and are present in widely different copy numbers within and between Leishmania strains. Homologou...
| Main Authors: | , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Oxford University Press
2002
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/35262 |
| _version_ | 1855514227316359168 |
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| author | Lambson, B. Barker, D.C. |
| author_browse | Barker, D.C. Lambson, B. |
| author_facet | Lambson, B. Barker, D.C. |
| author_sort | Lambson, B. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Leishmania minicircular deoxyribonucleic acid (DNA) is arranged into different classes according to sequence. These classes differ substantially in sequence, despite species- and genus-specific regions, and are present in widely different copy numbers within and between Leishmania strains. Homologous minicircles have been identified in different species of Leishmania by comparing sequences of known minicircles. However, it is possible to select for minicircles of the same class by amplifying Leishmania DNA with polymerase chain reaction primers from the conserved and variable regions. This approach was used with 2 different minicircle classes in the L. donovani complex. In all isolates tested it was possible to amplify minicircles of the selected class. |
| format | Journal Article |
| id | CGSpace35262 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2002 |
| publishDateRange | 2002 |
| publishDateSort | 2002 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | CGSpace352622024-08-27T12:27:42Z Homologous minicircles in Leishmania donovani Lambson, B. Barker, D.C. livestock research infectious diseases parasitology Leishmania minicircular deoxyribonucleic acid (DNA) is arranged into different classes according to sequence. These classes differ substantially in sequence, despite species- and genus-specific regions, and are present in widely different copy numbers within and between Leishmania strains. Homologous minicircles have been identified in different species of Leishmania by comparing sequences of known minicircles. However, it is possible to select for minicircles of the same class by amplifying Leishmania DNA with polymerase chain reaction primers from the conserved and variable regions. This approach was used with 2 different minicircle classes in the L. donovani complex. In all isolates tested it was possible to amplify minicircles of the selected class. 2002-04 2014-04-14T10:55:42Z 2014-04-14T10:55:42Z Journal Article https://hdl.handle.net/10568/35262 en Limited Access Oxford University Press Lambson, B. and Barker, D.C. 2002. Homologous minicircles in Leishmania donovani. Transactions of the Royal Society of Tropical Medicine and Hygiene 96(S1): 75-80 |
| spellingShingle | livestock research infectious diseases parasitology Lambson, B. Barker, D.C. Homologous minicircles in Leishmania donovani |
| title | Homologous minicircles in Leishmania donovani |
| title_full | Homologous minicircles in Leishmania donovani |
| title_fullStr | Homologous minicircles in Leishmania donovani |
| title_full_unstemmed | Homologous minicircles in Leishmania donovani |
| title_short | Homologous minicircles in Leishmania donovani |
| title_sort | homologous minicircles in leishmania donovani |
| topic | livestock research infectious diseases parasitology |
| url | https://hdl.handle.net/10568/35262 |
| work_keys_str_mv | AT lambsonb homologousminicirclesinleishmaniadonovani AT barkerdc homologousminicirclesinleishmaniadonovani |