The nucleotide sequence of the bovine interleukin-5-encoding cDNA
The nucleotide (nt) sequence encoding the bovine homologue of interleukin-5 (boIL-5) was determined. Total cDNA generated from stimulated bovine peripheral blood lymphocytes was used to amplify the boIL-5 gene using primers based on the 5' and 3' untranslated regions of the ovine IL-5 cDNA sequence....
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Elsevier
1996
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/35259 |
| _version_ | 1855526012579741696 |
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| author | Mertens, B. Gobright, E.I. Seow, H.F. |
| author_browse | Gobright, E.I. Mertens, B. Seow, H.F. |
| author_facet | Mertens, B. Gobright, E.I. Seow, H.F. |
| author_sort | Mertens, B. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The nucleotide (nt) sequence encoding the bovine homologue of interleukin-5 (boIL-5) was determined. Total cDNA generated from stimulated bovine peripheral blood lymphocytes was used to amplify the boIL-5 gene using primers based on the 5' and 3' untranslated regions of the ovine IL-5 cDNA sequence. The boIL-5 coding sequence is 405 by long, coding for a 15.2 kDa precursor protein of 134 amino acids (aa). Cleavage of a putative signal peptide of 19 aa yields a mature form of 13.1 kDa. Comparisons at the nt level revealed 96%, 81%, 74% and 73% identity with the ovine, human, mouse and rat IL-5 sequences, respectively, and 97%, 66%, 59% and 58% aa identity, respectively. |
| format | Journal Article |
| id | CGSpace35259 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1996 |
| publishDateRange | 1996 |
| publishDateSort | 1996 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace352592024-08-27T12:27:43Z The nucleotide sequence of the bovine interleukin-5-encoding cDNA Mertens, B. Gobright, E.I. Seow, H.F. research livestock cattle The nucleotide (nt) sequence encoding the bovine homologue of interleukin-5 (boIL-5) was determined. Total cDNA generated from stimulated bovine peripheral blood lymphocytes was used to amplify the boIL-5 gene using primers based on the 5' and 3' untranslated regions of the ovine IL-5 cDNA sequence. The boIL-5 coding sequence is 405 by long, coding for a 15.2 kDa precursor protein of 134 amino acids (aa). Cleavage of a putative signal peptide of 19 aa yields a mature form of 13.1 kDa. Comparisons at the nt level revealed 96%, 81%, 74% and 73% identity with the ovine, human, mouse and rat IL-5 sequences, respectively, and 97%, 66%, 59% and 58% aa identity, respectively. 1996-10 2014-04-14T10:55:41Z 2014-04-14T10:55:41Z Journal Article https://hdl.handle.net/10568/35259 en Limited Access Elsevier Mertens, B., Gobright, E., Seow, H.-F. 1996. The nucleotide sequence of the bovine interleukin-5-encoding cDNA. Gene 176 (1-2): 273 -274 |
| spellingShingle | research livestock cattle Mertens, B. Gobright, E.I. Seow, H.F. The nucleotide sequence of the bovine interleukin-5-encoding cDNA |
| title | The nucleotide sequence of the bovine interleukin-5-encoding cDNA |
| title_full | The nucleotide sequence of the bovine interleukin-5-encoding cDNA |
| title_fullStr | The nucleotide sequence of the bovine interleukin-5-encoding cDNA |
| title_full_unstemmed | The nucleotide sequence of the bovine interleukin-5-encoding cDNA |
| title_short | The nucleotide sequence of the bovine interleukin-5-encoding cDNA |
| title_sort | nucleotide sequence of the bovine interleukin 5 encoding cdna |
| topic | research livestock cattle |
| url | https://hdl.handle.net/10568/35259 |
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