Capturing the natural diversity of the human antibody response against vaccinia virus
The eradication of smallpox (variola) and the subsequent cessation of routine vaccination have left modern society vulnerable to bioterrorism employing this devastating contagious disease. The existing, licensed vaccines based on live vaccinia virus (VACV) are contraindicated for a substantial numbe...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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American Society for Microbiology
2011
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/35251 |
| _version_ | 1855526309931778048 |
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| author | Lantto, J. Hansen, M.H. Rasmussen, S.K. Steinaa, Lucilla Poulsen, T.R. Duggan, J. Dennis, M. Naylor, I. Easterbrook, L. Bregenholt, S. Haurum, J. Jensen, A. |
| author_browse | Bregenholt, S. Dennis, M. Duggan, J. Easterbrook, L. Hansen, M.H. Haurum, J. Jensen, A. Lantto, J. Naylor, I. Poulsen, T.R. Rasmussen, S.K. Steinaa, Lucilla |
| author_facet | Lantto, J. Hansen, M.H. Rasmussen, S.K. Steinaa, Lucilla Poulsen, T.R. Duggan, J. Dennis, M. Naylor, I. Easterbrook, L. Bregenholt, S. Haurum, J. Jensen, A. |
| author_sort | Lantto, J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The eradication of smallpox (variola) and the subsequent cessation of routine vaccination have left modern society vulnerable to bioterrorism employing this devastating contagious disease. The existing, licensed vaccines based on live vaccinia virus (VACV) are contraindicated for a substantial number of people, and prophylactic vaccination of large populations is not reasonable when there is little risk of exposure. Consequently, there is an emerging need to develop efficient and safe therapeutics to be used shortly before or after exposure, either alone or in combination with vaccination. We have characterized the human antibody response to smallpox vaccine (VACV Lister) in immunized volunteers and isolated a large number of VACV-specific antibodies that recognize a variety of different VACV antigens. Using this broad antibody panel, we have generated a fully human, recombinant analogue to plasma-derived vaccinia immunoglobulin (VIG), which mirrors the diversity and specificity of the human antibody immune response and offers the advantage of unlimited supply and reproducible specificity and activity. The recombinant VIG was found to display a high specific binding activity toward VACV antigens, potent in vitro VACV neutralizing activity, and a highly protective efficacy against VACV challenge in the mouse tail lesion model when given either prophylactically or therapeutically. Altogether, the results suggest that this compound has the potential to be used as an effective postexposure prophylaxis or treatment of disease caused by orthopoxviruses. |
| format | Journal Article |
| id | CGSpace35251 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2011 |
| publishDateRange | 2011 |
| publishDateSort | 2011 |
| publisher | American Society for Microbiology |
| publisherStr | American Society for Microbiology |
| record_format | dspace |
| spelling | CGSpace352512024-08-27T10:36:04Z Capturing the natural diversity of the human antibody response against vaccinia virus Lantto, J. Hansen, M.H. Rasmussen, S.K. Steinaa, Lucilla Poulsen, T.R. Duggan, J. Dennis, M. Naylor, I. Easterbrook, L. Bregenholt, S. Haurum, J. Jensen, A. animal diseases vaccines The eradication of smallpox (variola) and the subsequent cessation of routine vaccination have left modern society vulnerable to bioterrorism employing this devastating contagious disease. The existing, licensed vaccines based on live vaccinia virus (VACV) are contraindicated for a substantial number of people, and prophylactic vaccination of large populations is not reasonable when there is little risk of exposure. Consequently, there is an emerging need to develop efficient and safe therapeutics to be used shortly before or after exposure, either alone or in combination with vaccination. We have characterized the human antibody response to smallpox vaccine (VACV Lister) in immunized volunteers and isolated a large number of VACV-specific antibodies that recognize a variety of different VACV antigens. Using this broad antibody panel, we have generated a fully human, recombinant analogue to plasma-derived vaccinia immunoglobulin (VIG), which mirrors the diversity and specificity of the human antibody immune response and offers the advantage of unlimited supply and reproducible specificity and activity. The recombinant VIG was found to display a high specific binding activity toward VACV antigens, potent in vitro VACV neutralizing activity, and a highly protective efficacy against VACV challenge in the mouse tail lesion model when given either prophylactically or therapeutically. Altogether, the results suggest that this compound has the potential to be used as an effective postexposure prophylaxis or treatment of disease caused by orthopoxviruses. 2011-02-15 2014-04-14T10:55:38Z 2014-04-14T10:55:38Z Journal Article https://hdl.handle.net/10568/35251 en Open Access American Society for Microbiology Lantto, J., Hansen, M.H., Rasmussen, S.K., Steinaa, L., Poulsen, T.R., Duggan, J., Dennis, M., Naylor, I., Easterbrook, L., Bregenholt, S., Haurum, J. and Jensen, A. 2010. Capturing the natural diversity of the human antibody response against vaccinia virus 85(4): 1820 - 1833 |
| spellingShingle | animal diseases vaccines Lantto, J. Hansen, M.H. Rasmussen, S.K. Steinaa, Lucilla Poulsen, T.R. Duggan, J. Dennis, M. Naylor, I. Easterbrook, L. Bregenholt, S. Haurum, J. Jensen, A. Capturing the natural diversity of the human antibody response against vaccinia virus |
| title | Capturing the natural diversity of the human antibody response against vaccinia virus |
| title_full | Capturing the natural diversity of the human antibody response against vaccinia virus |
| title_fullStr | Capturing the natural diversity of the human antibody response against vaccinia virus |
| title_full_unstemmed | Capturing the natural diversity of the human antibody response against vaccinia virus |
| title_short | Capturing the natural diversity of the human antibody response against vaccinia virus |
| title_sort | capturing the natural diversity of the human antibody response against vaccinia virus |
| topic | animal diseases vaccines |
| url | https://hdl.handle.net/10568/35251 |
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