Use of SNP genotyping to determine pedigree and breed composition of dairy cattle in Kenya

High levels of inbreeding in East African dairy cattle are a potential concern because of use of a limited range of imported germplasm coupled with strong selection, especially by disease, and sparse performance recording. To address this, genetic relationships and breed composition in an admixed po...

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Autores principales: Gorbach, D.M., Makgahlela, M.L., Reecy, J.M., Kemp, Stephen J., Baltenweck, Isabelle, Ouma, R., Okeyo Mwai, Ally, Marshall, Karen, Murdoch, B., Moore, S., Rothschild, Max F.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Wiley 2010
Materias:
Acceso en línea:https://hdl.handle.net/10568/3496
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author Gorbach, D.M.
Makgahlela, M.L.
Reecy, J.M.
Kemp, Stephen J.
Baltenweck, Isabelle
Ouma, R.
Okeyo Mwai, Ally
Marshall, Karen
Murdoch, B.
Moore, S.
Rothschild, Max F.
author_browse Baltenweck, Isabelle
Gorbach, D.M.
Kemp, Stephen J.
Makgahlela, M.L.
Marshall, Karen
Moore, S.
Murdoch, B.
Okeyo Mwai, Ally
Ouma, R.
Reecy, J.M.
Rothschild, Max F.
author_facet Gorbach, D.M.
Makgahlela, M.L.
Reecy, J.M.
Kemp, Stephen J.
Baltenweck, Isabelle
Ouma, R.
Okeyo Mwai, Ally
Marshall, Karen
Murdoch, B.
Moore, S.
Rothschild, Max F.
author_sort Gorbach, D.M.
collection Repository of Agricultural Research Outputs (CGSpace)
description High levels of inbreeding in East African dairy cattle are a potential concern because of use of a limited range of imported germplasm coupled with strong selection, especially by disease, and sparse performance recording. To address this, genetic relationships and breed composition in an admixed population of Kenyan dairy cattle were estimated by means of a 50K SNP scan. Genomic DNA from 3 worldwide Holstein and 20 Kenyan bulls, 71 putative cow-calf pairs, 25 cows from a large ranch and 5 other Kenyan animals were genotyped for 37 238 informative SNPs. Sires were predicted and 89% of putative dam-calf relationships were supported by genotype data. Animals were clustered with the HapMap population using Structure software to assess breed composition. Cows from a large ranch primarily clustered with Holsteins, while animals from smaller farms were generally crosses between Holstein and Guernsey. Coefficients of relatedness were estimated and showed evidence of heavy use of one AI bull. We conclude that little native germplasm exists within the genotyped populations and mostly European ancestry remains.
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spelling CGSpace34962023-09-30T10:58:31Z Use of SNP genotyping to determine pedigree and breed composition of dairy cattle in Kenya Gorbach, D.M. Makgahlela, M.L. Reecy, J.M. Kemp, Stephen J. Baltenweck, Isabelle Ouma, R. Okeyo Mwai, Ally Marshall, Karen Murdoch, B. Moore, S. Rothschild, Max F. dairies cattle High levels of inbreeding in East African dairy cattle are a potential concern because of use of a limited range of imported germplasm coupled with strong selection, especially by disease, and sparse performance recording. To address this, genetic relationships and breed composition in an admixed population of Kenyan dairy cattle were estimated by means of a 50K SNP scan. Genomic DNA from 3 worldwide Holstein and 20 Kenyan bulls, 71 putative cow-calf pairs, 25 cows from a large ranch and 5 other Kenyan animals were genotyped for 37 238 informative SNPs. Sires were predicted and 89% of putative dam-calf relationships were supported by genotype data. Animals were clustered with the HapMap population using Structure software to assess breed composition. Cows from a large ranch primarily clustered with Holsteins, while animals from smaller farms were generally crosses between Holstein and Guernsey. Coefficients of relatedness were estimated and showed evidence of heavy use of one AI bull. We conclude that little native germplasm exists within the genotyped populations and mostly European ancestry remains. 2010-10 2011-04-17T11:28:54Z 2011-04-17T11:28:54Z Journal Article https://hdl.handle.net/10568/3496 en Open Access Wiley Gorbach, D.M., Makgahlela, M.L., Reecy, J.M., Kemp, S.J., Baltenweck, I., Ouma, R., Okeyo, A.M., Marshall, K., Murdoch, B., Moore, S. and Rothschild, M.F. 2010. Use of SNP genotyping to determine pedigree and breed composition of dairy cattle in Kenya. Journal of Animal Breeding and Genetics 127(5):348-351.
spellingShingle dairies
cattle
Gorbach, D.M.
Makgahlela, M.L.
Reecy, J.M.
Kemp, Stephen J.
Baltenweck, Isabelle
Ouma, R.
Okeyo Mwai, Ally
Marshall, Karen
Murdoch, B.
Moore, S.
Rothschild, Max F.
Use of SNP genotyping to determine pedigree and breed composition of dairy cattle in Kenya
title Use of SNP genotyping to determine pedigree and breed composition of dairy cattle in Kenya
title_full Use of SNP genotyping to determine pedigree and breed composition of dairy cattle in Kenya
title_fullStr Use of SNP genotyping to determine pedigree and breed composition of dairy cattle in Kenya
title_full_unstemmed Use of SNP genotyping to determine pedigree and breed composition of dairy cattle in Kenya
title_short Use of SNP genotyping to determine pedigree and breed composition of dairy cattle in Kenya
title_sort use of snp genotyping to determine pedigree and breed composition of dairy cattle in kenya
topic dairies
cattle
url https://hdl.handle.net/10568/3496
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