Application of septenary multiplex PCR detection for some animal components in sheep and goat meat products
Septenary multiplex PCR method and protocol to identify DNAs of pig, cow, sheep, goat, chicken, horse and yak was optimized and developed. Sensitivity of the three species (cow, yak and goat) in the septenary multiplex PCR system was analysed. 10 products made of sheep and goat meat were detected. T...
| Autores principales: | , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2010
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| Acceso en línea: | https://hdl.handle.net/10568/3486 |
| _version_ | 1855529043624984576 |
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| author | Feng, H. Han, J. |
| author_browse | Feng, H. Han, J. |
| author_facet | Feng, H. Han, J. |
| author_sort | Feng, H. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Septenary multiplex PCR method and protocol to identify DNAs of pig, cow, sheep, goat, chicken, horse and yak was optimized and developed. Sensitivity of the three species (cow, yak and goat) in the septenary multiplex PCR system was analysed. 10 products made of sheep and goat meat were detected. The results showed that routine agarose gel electrophoresis was sufficient to differentiate the multiplex PCR products ranging from 175 to 517 bp and differing by at least 41 bp each other, indicating that this septenary multiplex PCR method could be used for rapid and accurate identification of the seven species and for three species (cow, yak and goat) at sensitivity of 2.5 ng DNA. Among the 10 products examined using this protocol, 2 products were found to have mixed with some beef or completely adulterated by beef. |
| format | Journal Article |
| id | CGSpace3486 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2010 |
| publishDateRange | 2010 |
| publishDateSort | 2010 |
| record_format | dspace |
| spelling | CGSpace34862022-01-29T17:19:30Z Application of septenary multiplex PCR detection for some animal components in sheep and goat meat products Feng, H. Han, J. Septenary multiplex PCR method and protocol to identify DNAs of pig, cow, sheep, goat, chicken, horse and yak was optimized and developed. Sensitivity of the three species (cow, yak and goat) in the septenary multiplex PCR system was analysed. 10 products made of sheep and goat meat were detected. The results showed that routine agarose gel electrophoresis was sufficient to differentiate the multiplex PCR products ranging from 175 to 517 bp and differing by at least 41 bp each other, indicating that this septenary multiplex PCR method could be used for rapid and accurate identification of the seven species and for three species (cow, yak and goat) at sensitivity of 2.5 ng DNA. Among the 10 products examined using this protocol, 2 products were found to have mixed with some beef or completely adulterated by beef. 2010-03-19 2011-04-17T11:07:21Z 2011-04-17T11:07:21Z Journal Article https://hdl.handle.net/10568/3486 en Limited Access Feng, H. and Han, J. 2010. Application of septenary multiplex PCR detection for some animal components in sheep and goat meat products. China Animal Husbandry and Veterinary Medicine 37(9):85-90. |
| spellingShingle | Feng, H. Han, J. Application of septenary multiplex PCR detection for some animal components in sheep and goat meat products |
| title | Application of septenary multiplex PCR detection for some animal components in sheep and goat meat products |
| title_full | Application of septenary multiplex PCR detection for some animal components in sheep and goat meat products |
| title_fullStr | Application of septenary multiplex PCR detection for some animal components in sheep and goat meat products |
| title_full_unstemmed | Application of septenary multiplex PCR detection for some animal components in sheep and goat meat products |
| title_short | Application of septenary multiplex PCR detection for some animal components in sheep and goat meat products |
| title_sort | application of septenary multiplex pcr detection for some animal components in sheep and goat meat products |
| url | https://hdl.handle.net/10568/3486 |
| work_keys_str_mv | AT fengh applicationofseptenarymultiplexpcrdetectionforsomeanimalcomponentsinsheepandgoatmeatproducts AT hanj applicationofseptenarymultiplexpcrdetectionforsomeanimalcomponentsinsheepandgoatmeatproducts |