Characterization of Ugandan sweetpotato germplasm using fluorescent labeled simple sequence repeat markers
The genetic relationships among 192 superior, high-yielding, and disease-resistant sweetpotato [Ipomoea batatas (L.) Lam] accessions from the Ugandan germplasm collection were analyzed using 10 fluorescent labeled simple sequence repeat (SSR) markers. Relatedness among the genotypes was estimated us...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2010
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| Acceso en línea: | https://hdl.handle.net/10568/3480 |
| _version_ | 1855535229700145152 |
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| author | Yada, B. Tukamuhabwe, Phineas Wanjala, Bramwel W. Kim, D.J. Skilton, Robert A. Alajo, B.A. Mwanga, Robert O.M. |
| author_browse | Alajo, B.A. Kim, D.J. Mwanga, Robert O.M. Skilton, Robert A. Tukamuhabwe, Phineas Wanjala, Bramwel W. Yada, B. |
| author_facet | Yada, B. Tukamuhabwe, Phineas Wanjala, Bramwel W. Kim, D.J. Skilton, Robert A. Alajo, B.A. Mwanga, Robert O.M. |
| author_sort | Yada, B. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The genetic relationships among 192 superior, high-yielding, and disease-resistant sweetpotato [Ipomoea batatas (L.) Lam] accessions from the Ugandan germplasm collection were analyzed using 10 fluorescent labeled simple sequence repeat (SSR) markers. Relatedness among the genotypes was estimated using the Nei and Li genetic distance coefficient, cluster analysis and principle component analysis methods of NTSYS-pc software. The polymorphic information content of the SSR markers used in this study ranged from 0.23 to 0.76 for loci IB-S07 and [B-R12, respectively, with a mean value of 0.62. The number of polymorphic alleles detected per locus ranged from two to six with a mean of four, a confirmation of the effectiveness of microsatellite detection on an automated ABI 3730 sequencer. The mean pairwise genetic distance among the 192 genotypes was 0.57, an indication of moderately high genetic diversity. Cluster analysis divided the accessions into four major groups with no relationship to the district of origin. Two sets of duplicates were identified through SSR genotyping in this study. Up to 190 distinct accessions for use as potential parental genotypes in hybridization schemes for cultivar development in the region were identified. |
| format | Journal Article |
| id | CGSpace3480 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2010 |
| publishDateRange | 2010 |
| publishDateSort | 2010 |
| record_format | dspace |
| spelling | CGSpace34802024-03-06T10:16:43Z Characterization of Ugandan sweetpotato germplasm using fluorescent labeled simple sequence repeat markers Yada, B. Tukamuhabwe, Phineas Wanjala, Bramwel W. Kim, D.J. Skilton, Robert A. Alajo, B.A. Mwanga, Robert O.M. The genetic relationships among 192 superior, high-yielding, and disease-resistant sweetpotato [Ipomoea batatas (L.) Lam] accessions from the Ugandan germplasm collection were analyzed using 10 fluorescent labeled simple sequence repeat (SSR) markers. Relatedness among the genotypes was estimated using the Nei and Li genetic distance coefficient, cluster analysis and principle component analysis methods of NTSYS-pc software. The polymorphic information content of the SSR markers used in this study ranged from 0.23 to 0.76 for loci IB-S07 and [B-R12, respectively, with a mean value of 0.62. The number of polymorphic alleles detected per locus ranged from two to six with a mean of four, a confirmation of the effectiveness of microsatellite detection on an automated ABI 3730 sequencer. The mean pairwise genetic distance among the 192 genotypes was 0.57, an indication of moderately high genetic diversity. Cluster analysis divided the accessions into four major groups with no relationship to the district of origin. Two sets of duplicates were identified through SSR genotyping in this study. Up to 190 distinct accessions for use as potential parental genotypes in hybridization schemes for cultivar development in the region were identified. 2010-02-01 2011-04-17T10:46:11Z 2011-04-17T10:46:11Z Journal Article https://hdl.handle.net/10568/3480 en Limited Access Yada, B., Tukamuhabwa, P., Wanjala, B., Kim, D.J., Skilton, R.A., Alajo, B.A. and Mwanga, R.O.M. 2010. Characterization of Ugandan sweetpotato germplasm using fluorescent labeled simple sequence repeat markers. HortScience 45(2):225-230. |
| spellingShingle | Yada, B. Tukamuhabwe, Phineas Wanjala, Bramwel W. Kim, D.J. Skilton, Robert A. Alajo, B.A. Mwanga, Robert O.M. Characterization of Ugandan sweetpotato germplasm using fluorescent labeled simple sequence repeat markers |
| title | Characterization of Ugandan sweetpotato germplasm using fluorescent labeled simple sequence repeat markers |
| title_full | Characterization of Ugandan sweetpotato germplasm using fluorescent labeled simple sequence repeat markers |
| title_fullStr | Characterization of Ugandan sweetpotato germplasm using fluorescent labeled simple sequence repeat markers |
| title_full_unstemmed | Characterization of Ugandan sweetpotato germplasm using fluorescent labeled simple sequence repeat markers |
| title_short | Characterization of Ugandan sweetpotato germplasm using fluorescent labeled simple sequence repeat markers |
| title_sort | characterization of ugandan sweetpotato germplasm using fluorescent labeled simple sequence repeat markers |
| url | https://hdl.handle.net/10568/3480 |
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