Genetic characterization of infectious bursal disease virus isolates in Ethiopia
The objective of the investigation was to characterize infectious bursal disease viruses (IBDV) circulating in commercial and breeding poultry farms in Ethiopia between 2009 and 2011. The nucleotide and deduced amino acid sequence for VP2 hypervariable region of ten IBDVs were determined by RT-PCR,...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2014
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/33888 |
| _version_ | 1855542778424983552 |
|---|---|
| author | Jenberie, S. Lynch, Stacey E. Kebede, F. Christley, Robert M. Gelaye, E. Negussie, H. Asmare, K. Ayelet, G. |
| author_browse | Asmare, K. Ayelet, G. Christley, Robert M. Gelaye, E. Jenberie, S. Kebede, F. Lynch, Stacey E. Negussie, H. |
| author_facet | Jenberie, S. Lynch, Stacey E. Kebede, F. Christley, Robert M. Gelaye, E. Negussie, H. Asmare, K. Ayelet, G. |
| author_sort | Jenberie, S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The objective of the investigation was to characterize infectious bursal disease viruses (IBDV) circulating in commercial and breeding poultry farms in Ethiopia between 2009 and 2011. The nucleotide and deduced amino acid sequence for VP2 hypervariable region of ten IBDVs were determined by RT-PCR, sequenced and compared to well characterised IBDV isolates worldwide. IBDV genetic material was amplified directly from bursa or cell passaged material. Phylogenetically, Ethiopian IBDVs represented two genetic lineages: very virulent (vv) IBDVs or variants of the classical attenuated vaccine strain (D78). The nucleotide identity between Ethiopian vvIBDVs ranged between 0% to 2.6%. Ethiopian vvIBDVs are clustered phylogenetically with the African IBDV genetic lineage, independent of the Asian/European lineage. This report demonstrates the circulation of vvIBDV in commercial and breeding poultry farms in Ethiopia. |
| format | Journal Article |
| id | CGSpace33888 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace338882024-05-01T08:20:06Z Genetic characterization of infectious bursal disease virus isolates in Ethiopia Jenberie, S. Lynch, Stacey E. Kebede, F. Christley, Robert M. Gelaye, E. Negussie, H. Asmare, K. Ayelet, G. animal diseases genetics The objective of the investigation was to characterize infectious bursal disease viruses (IBDV) circulating in commercial and breeding poultry farms in Ethiopia between 2009 and 2011. The nucleotide and deduced amino acid sequence for VP2 hypervariable region of ten IBDVs were determined by RT-PCR, sequenced and compared to well characterised IBDV isolates worldwide. IBDV genetic material was amplified directly from bursa or cell passaged material. Phylogenetically, Ethiopian IBDVs represented two genetic lineages: very virulent (vv) IBDVs or variants of the classical attenuated vaccine strain (D78). The nucleotide identity between Ethiopian vvIBDVs ranged between 0% to 2.6%. Ethiopian vvIBDVs are clustered phylogenetically with the African IBDV genetic lineage, independent of the Asian/European lineage. This report demonstrates the circulation of vvIBDV in commercial and breeding poultry farms in Ethiopia. 2014-02 2013-11-03T13:35:22Z 2013-11-03T13:35:22Z Journal Article https://hdl.handle.net/10568/33888 en Open Access Elsevier Jenberie, S., Lynch, S.E., Kebede, F., Christley, R., Gelaye, E., Negussie, H., Asmare, K. and Ayelet, G. 2013. Genetic characterization of infectious bursal disease virus isolates in Ethiopia. Acta Tropica 130: 39-43. |
| spellingShingle | animal diseases genetics Jenberie, S. Lynch, Stacey E. Kebede, F. Christley, Robert M. Gelaye, E. Negussie, H. Asmare, K. Ayelet, G. Genetic characterization of infectious bursal disease virus isolates in Ethiopia |
| title | Genetic characterization of infectious bursal disease virus isolates in Ethiopia |
| title_full | Genetic characterization of infectious bursal disease virus isolates in Ethiopia |
| title_fullStr | Genetic characterization of infectious bursal disease virus isolates in Ethiopia |
| title_full_unstemmed | Genetic characterization of infectious bursal disease virus isolates in Ethiopia |
| title_short | Genetic characterization of infectious bursal disease virus isolates in Ethiopia |
| title_sort | genetic characterization of infectious bursal disease virus isolates in ethiopia |
| topic | animal diseases genetics |
| url | https://hdl.handle.net/10568/33888 |
| work_keys_str_mv | AT jenberies geneticcharacterizationofinfectiousbursaldiseasevirusisolatesinethiopia AT lynchstaceye geneticcharacterizationofinfectiousbursaldiseasevirusisolatesinethiopia AT kebedef geneticcharacterizationofinfectiousbursaldiseasevirusisolatesinethiopia AT christleyrobertm geneticcharacterizationofinfectiousbursaldiseasevirusisolatesinethiopia AT gelayee geneticcharacterizationofinfectiousbursaldiseasevirusisolatesinethiopia AT negussieh geneticcharacterizationofinfectiousbursaldiseasevirusisolatesinethiopia AT asmarek geneticcharacterizationofinfectiousbursaldiseasevirusisolatesinethiopia AT ayeletg geneticcharacterizationofinfectiousbursaldiseasevirusisolatesinethiopia |