Characterization of trypanosome isolates from naturally infected horses on a farm in Kenya
Following an outbreak of trypanosomosis in horses on a farm in Kenya, 18 trypanosome isolates were collected from the infected animals over a period of one and a half years and cryopreserved for characterization. The characterization was done on the basis of morphology using Giemsa-stained blood and...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Elsevier
2000
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/33252 |
| _version_ | 1855533158660833280 |
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| author | Kihurani, D.O. Masake, R.A. Nantulya, V.M. Mbiuki, S.M. |
| author_browse | Kihurani, D.O. Masake, R.A. Mbiuki, S.M. Nantulya, V.M. |
| author_facet | Kihurani, D.O. Masake, R.A. Nantulya, V.M. Mbiuki, S.M. |
| author_sort | Kihurani, D.O. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Following an outbreak of trypanosomosis in horses on a farm in Kenya, 18 trypanosome isolates were collected from the infected animals over a period of one and a half years and cryopreserved for characterization. The characterization was done on the basis of morphology using Giemsa-stained blood and buffy coat smears, infectivity to mice, recombinant DNA hybridization, and chromosome separation by orthogonal field alternation gel electrophoresis (OFAGE). Morphologically, all the trypanosome isolates were identified as belonging to the subgenus Nannomonas, and a total of 16 out of the 18 isolates grew in mice. Using the recombinant DNA hybridization technique, the isolates were further classified as the 'savannah' type of Trypanosoma congolense. Furthermore, chromosome separation by OFAGE, carried out on six clones derived from different isolates, exhibited a profile characteristic of T. congolense, 'savannah' type. However, there were differences in the number and positions of the medium-sized and minichromosomes indicating a diversity of serodemes within the isolates. Hence the infecting trypanosomes in this disease outbreak were T. congolense, 'savannah' type, and comprised several serodemes or strains. |
| format | Journal Article |
| id | CGSpace33252 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2000 |
| publishDateRange | 2000 |
| publishDateSort | 2000 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace332522024-04-25T06:01:25Z Characterization of trypanosome isolates from naturally infected horses on a farm in Kenya Kihurani, D.O. Masake, R.A. Nantulya, V.M. Mbiuki, S.M. trypanosoma congolense horses diagnosis protozoa animal morphology trypanosoma brucei cloning dna hybridization electrophoresis Following an outbreak of trypanosomosis in horses on a farm in Kenya, 18 trypanosome isolates were collected from the infected animals over a period of one and a half years and cryopreserved for characterization. The characterization was done on the basis of morphology using Giemsa-stained blood and buffy coat smears, infectivity to mice, recombinant DNA hybridization, and chromosome separation by orthogonal field alternation gel electrophoresis (OFAGE). Morphologically, all the trypanosome isolates were identified as belonging to the subgenus Nannomonas, and a total of 16 out of the 18 isolates grew in mice. Using the recombinant DNA hybridization technique, the isolates were further classified as the 'savannah' type of Trypanosoma congolense. Furthermore, chromosome separation by OFAGE, carried out on six clones derived from different isolates, exhibited a profile characteristic of T. congolense, 'savannah' type. However, there were differences in the number and positions of the medium-sized and minichromosomes indicating a diversity of serodemes within the isolates. Hence the infecting trypanosomes in this disease outbreak were T. congolense, 'savannah' type, and comprised several serodemes or strains. 2000-04 2013-07-03T05:26:18Z 2013-07-03T05:26:18Z Journal Article https://hdl.handle.net/10568/33252 en Limited Access Elsevier Veterinary Parasitology;89(3): 173-185 |
| spellingShingle | trypanosoma congolense horses diagnosis protozoa animal morphology trypanosoma brucei cloning dna hybridization electrophoresis Kihurani, D.O. Masake, R.A. Nantulya, V.M. Mbiuki, S.M. Characterization of trypanosome isolates from naturally infected horses on a farm in Kenya |
| title | Characterization of trypanosome isolates from naturally infected horses on a farm in Kenya |
| title_full | Characterization of trypanosome isolates from naturally infected horses on a farm in Kenya |
| title_fullStr | Characterization of trypanosome isolates from naturally infected horses on a farm in Kenya |
| title_full_unstemmed | Characterization of trypanosome isolates from naturally infected horses on a farm in Kenya |
| title_short | Characterization of trypanosome isolates from naturally infected horses on a farm in Kenya |
| title_sort | characterization of trypanosome isolates from naturally infected horses on a farm in kenya |
| topic | trypanosoma congolense horses diagnosis protozoa animal morphology trypanosoma brucei cloning dna hybridization electrophoresis |
| url | https://hdl.handle.net/10568/33252 |
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