Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens
East Coast fever (ECF) in cattle is caused by the tick-borne protozoan parasite Theileria parva. The major sporozoite surface antigen of T. parva (p67) is an important candidate for inclusion in a subunit vaccine. Recently, we reported the expression and production of different parts of p67 as fusio...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Elsevier
2004
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/33234 |
| _version_ | 1855524317043884032 |
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| author | Kaba, S.A. Schaap, D. Roode, E.C. Nene, Vishvanath M. Musoke, A.J. Vlak, J.M. Oers, M.M. van |
| author_browse | Kaba, S.A. Musoke, A.J. Nene, Vishvanath M. Oers, M.M. van Roode, E.C. Schaap, D. Vlak, J.M. |
| author_facet | Kaba, S.A. Schaap, D. Roode, E.C. Nene, Vishvanath M. Musoke, A.J. Vlak, J.M. Oers, M.M. van |
| author_sort | Kaba, S.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | East Coast fever (ECF) in cattle is caused by the tick-borne protozoan parasite Theileria parva. The major sporozoite surface antigen of T. parva (p67) is an important candidate for inclusion in a subunit vaccine. Recently, we reported the expression and production of different parts of p67 as fusions to either GFP or to the baculovirus GP64 envelope glycoprotein in insect cells, which resulted in stable proteins recognized by a monoclonal specific for native p67. The immunogenicity of these fusion proteins was examined in out-bred mice and cattle. In mice, the full length p67 molecule without its signal peptide and transmembrane region, but fused to GFP (GFP:p67[Delta]SS) was the best immunogen followed by the C-terminus of p67 fused to GP64 (GP64:p67C). These two immunogens also provoked a high level of sero-conversion in cattle when formulated in a water-in-oil or saponin-derived adjuvant with only 100[mu]g of protein and a single booster. The vaccine-elicited antibodies efficiently inhibited the infectivity of T. parva sporozoites in in vitro neutralization assays. This study demonstrated that these new baculovirus-derived p67 vaccines were highly immunogenic, and that in combination with a suitable adjuvant, they have a clear potential to induce protective immunity in cattle. |
| format | Journal Article |
| id | CGSpace33234 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2004 |
| publishDateRange | 2004 |
| publishDateSort | 2004 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace332342024-05-01T08:17:10Z Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens Kaba, S.A. Schaap, D. Roode, E.C. Nene, Vishvanath M. Musoke, A.J. Vlak, J.M. Oers, M.M. van east coast fever theileria parva vaccines immunogenetics antigens baculoviridae [invertebrate viruses] East Coast fever (ECF) in cattle is caused by the tick-borne protozoan parasite Theileria parva. The major sporozoite surface antigen of T. parva (p67) is an important candidate for inclusion in a subunit vaccine. Recently, we reported the expression and production of different parts of p67 as fusions to either GFP or to the baculovirus GP64 envelope glycoprotein in insect cells, which resulted in stable proteins recognized by a monoclonal specific for native p67. The immunogenicity of these fusion proteins was examined in out-bred mice and cattle. In mice, the full length p67 molecule without its signal peptide and transmembrane region, but fused to GFP (GFP:p67[Delta]SS) was the best immunogen followed by the C-terminus of p67 fused to GP64 (GP64:p67C). These two immunogens also provoked a high level of sero-conversion in cattle when formulated in a water-in-oil or saponin-derived adjuvant with only 100[mu]g of protein and a single booster. The vaccine-elicited antibodies efficiently inhibited the infectivity of T. parva sporozoites in in vitro neutralization assays. This study demonstrated that these new baculovirus-derived p67 vaccines were highly immunogenic, and that in combination with a suitable adjuvant, they have a clear potential to induce protective immunity in cattle. 2004-05 2013-07-03T05:26:16Z 2013-07-03T05:26:16Z Journal Article https://hdl.handle.net/10568/33234 en Limited Access Elsevier Veterinary Parasitology;121(1-2): 53-64 |
| spellingShingle | east coast fever theileria parva vaccines immunogenetics antigens baculoviridae [invertebrate viruses] Kaba, S.A. Schaap, D. Roode, E.C. Nene, Vishvanath M. Musoke, A.J. Vlak, J.M. Oers, M.M. van Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens |
| title | Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens |
| title_full | Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens |
| title_fullStr | Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens |
| title_full_unstemmed | Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens |
| title_short | Improved immunogenicity of novel baculovirus-derived Theileria parva p67 subunit antigens |
| title_sort | improved immunogenicity of novel baculovirus derived theileria parva p67 subunit antigens |
| topic | east coast fever theileria parva vaccines immunogenetics antigens baculoviridae [invertebrate viruses] |
| url | https://hdl.handle.net/10568/33234 |
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