The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum
In order to define whether the variable antigenic type RoTat 1.2 is restricted to Trypansoma evansi and could be used as antigen in serological tests to differentiate T. evansi from Trypansoma equiperdum, the appearance of RoTat 1.2-specific antibodies in rabbits, experimentally infected with T. eva...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2003
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/33228 |
| _version_ | 1855525372803678208 |
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| author | Claes, F. Verloo, D. Waal, D.T. de Majiwa, Phelix A.O. Baltz, T. Goddeeris, Bruno M. Büscher, Philippe |
| author_browse | Baltz, T. Büscher, Philippe Claes, F. Goddeeris, Bruno M. Majiwa, Phelix A.O. Verloo, D. Waal, D.T. de |
| author_facet | Claes, F. Verloo, D. Waal, D.T. de Majiwa, Phelix A.O. Baltz, T. Goddeeris, Bruno M. Büscher, Philippe |
| author_sort | Claes, F. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In order to define whether the variable antigenic type RoTat 1.2 is restricted to Trypansoma evansi and could be used as antigen in serological tests to differentiate T. evansi from Trypansoma equiperdum, the appearance of RoTat 1.2-specific antibodies in rabbits, experimentally infected with T. evansi and T. equiperdum, respectively, was analyzed. Ten strains of T. evansi and 11 strains of T. equiperdum originating from Asia, Europe, Africa and Latin America were tested. Rabbit pre-infection sera and sera of days 7, 14, 25, 35 post-infection (p.i.) were analyzed for the presence of antibodies reactive with RoTat 1.2 in immune trypanolysis, ELISA/T. evansi and CATT/T. evansi. Within the duration of the infection (maximum 35 days), all T. evansi as well as 9 out of 11 T. equiperdum infected rabbits became positive in all these tests. The rabbits infected with T. equiperdum OVI and BoTat 1.1 (Morocco) remained negative in the immune trypanolysis test although the latter rabbit became positive in the CATT/T. evansi and ELISA/T. evansi. On the contrary, both rabbits were positive in immune trypanolysis when tested against their respective infecting population.From these data, we conclude that most T. equiperdum strains express isoVATs of RoTat 1.2. This explains, in part, why antibody tests based on T. evansi RoTat 1.2 cannot reliably distinguish between infections caused by T. evansi and those caused by T. equiperdum unless it can be proven that most described T. equiperdum are actually misclassified T. evansi. |
| format | Journal Article |
| id | CGSpace33228 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2003 |
| publishDateRange | 2003 |
| publishDateSort | 2003 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace332282024-04-25T06:00:57Z The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum Claes, F. Verloo, D. Waal, D.T. de Majiwa, Phelix A.O. Baltz, T. Goddeeris, Bruno M. Büscher, Philippe trypanosoma eqiperdum trypanosoma evansi antigens elisa glycoprotein antibodies rabbits In order to define whether the variable antigenic type RoTat 1.2 is restricted to Trypansoma evansi and could be used as antigen in serological tests to differentiate T. evansi from Trypansoma equiperdum, the appearance of RoTat 1.2-specific antibodies in rabbits, experimentally infected with T. evansi and T. equiperdum, respectively, was analyzed. Ten strains of T. evansi and 11 strains of T. equiperdum originating from Asia, Europe, Africa and Latin America were tested. Rabbit pre-infection sera and sera of days 7, 14, 25, 35 post-infection (p.i.) were analyzed for the presence of antibodies reactive with RoTat 1.2 in immune trypanolysis, ELISA/T. evansi and CATT/T. evansi. Within the duration of the infection (maximum 35 days), all T. evansi as well as 9 out of 11 T. equiperdum infected rabbits became positive in all these tests. The rabbits infected with T. equiperdum OVI and BoTat 1.1 (Morocco) remained negative in the immune trypanolysis test although the latter rabbit became positive in the CATT/T. evansi and ELISA/T. evansi. On the contrary, both rabbits were positive in immune trypanolysis when tested against their respective infecting population.From these data, we conclude that most T. equiperdum strains express isoVATs of RoTat 1.2. This explains, in part, why antibody tests based on T. evansi RoTat 1.2 cannot reliably distinguish between infections caused by T. evansi and those caused by T. equiperdum unless it can be proven that most described T. equiperdum are actually misclassified T. evansi. 2003-10 2013-07-03T05:26:15Z 2013-07-03T05:26:15Z Journal Article https://hdl.handle.net/10568/33228 en Limited Access Elsevier Veterinary Parasitology;116(3): 209-216 |
| spellingShingle | trypanosoma eqiperdum trypanosoma evansi antigens elisa glycoprotein antibodies rabbits Claes, F. Verloo, D. Waal, D.T. de Majiwa, Phelix A.O. Baltz, T. Goddeeris, Bruno M. Büscher, Philippe The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum |
| title | The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum |
| title_full | The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum |
| title_fullStr | The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum |
| title_full_unstemmed | The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum |
| title_short | The expression of RoTat 1.2 variable surface glycoprotein (VSG) in Trypanosoma evansi and T. equiperdum |
| title_sort | expression of rotat 1 2 variable surface glycoprotein vsg in trypanosoma evansi and t equiperdum |
| topic | trypanosoma eqiperdum trypanosoma evansi antigens elisa glycoprotein antibodies rabbits |
| url | https://hdl.handle.net/10568/33228 |
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