Trypanosome non-specific IgM antibodies detected in serum of Trypanosoma congolense-infected cattle are polyreactive
Serum Ig from Trypanosoma congolense-infected cattle were affinity-purified using immobilised trypanosome or non-trypanosome antigens (beta-galactosidase, cytochrome C and ferritin). The bound and unbound IgG and IgM fractions were collected and tested in ELISA for reactivity to each antigen. The re...
| Main Authors: | , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
1999
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/33222 |
| _version_ | 1855524357118361600 |
|---|---|
| author | Buza, J. Naessens, Jan |
| author_browse | Buza, J. Naessens, Jan |
| author_facet | Buza, J. Naessens, Jan |
| author_sort | Buza, J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Serum Ig from Trypanosoma congolense-infected cattle were affinity-purified using immobilised trypanosome or non-trypanosome antigens (beta-galactosidase, cytochrome C and ferritin). The bound and unbound IgG and IgM fractions were collected and tested in ELISA for reactivity to each antigen. The results indicated that the presence of reactivity to non-parasite antigens in serum of infected cattle is due to polyreactive IgM antibodies. However, the IgG fraction only bound to trypanosome antigens and was only present in post-infection sera, indicating that it was indiced by the infecting trypanosomes. Since the polyreactive IgM antibodies were also present in pre-infection sera, it is probable that they were natural antibodies that were not induced but only amplified by the trypanosome infection. |
| format | Journal Article |
| id | CGSpace33222 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1999 |
| publishDateRange | 1999 |
| publishDateSort | 1999 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace332222024-05-01T08:17:11Z Trypanosome non-specific IgM antibodies detected in serum of Trypanosoma congolense-infected cattle are polyreactive Buza, J. Naessens, Jan trypanosoma congolense antibodies serum trypanosomiasis cattle antigens Serum Ig from Trypanosoma congolense-infected cattle were affinity-purified using immobilised trypanosome or non-trypanosome antigens (beta-galactosidase, cytochrome C and ferritin). The bound and unbound IgG and IgM fractions were collected and tested in ELISA for reactivity to each antigen. The results indicated that the presence of reactivity to non-parasite antigens in serum of infected cattle is due to polyreactive IgM antibodies. However, the IgG fraction only bound to trypanosome antigens and was only present in post-infection sera, indicating that it was indiced by the infecting trypanosomes. Since the polyreactive IgM antibodies were also present in pre-infection sera, it is probable that they were natural antibodies that were not induced but only amplified by the trypanosome infection. 1999-07 2013-07-03T05:26:15Z 2013-07-03T05:26:15Z Journal Article https://hdl.handle.net/10568/33222 en Limited Access Elsevier Veterinary Immunology and Immunopathology;69 (1): 1-9 |
| spellingShingle | trypanosoma congolense antibodies serum trypanosomiasis cattle antigens Buza, J. Naessens, Jan Trypanosome non-specific IgM antibodies detected in serum of Trypanosoma congolense-infected cattle are polyreactive |
| title | Trypanosome non-specific IgM antibodies detected in serum of Trypanosoma congolense-infected cattle are polyreactive |
| title_full | Trypanosome non-specific IgM antibodies detected in serum of Trypanosoma congolense-infected cattle are polyreactive |
| title_fullStr | Trypanosome non-specific IgM antibodies detected in serum of Trypanosoma congolense-infected cattle are polyreactive |
| title_full_unstemmed | Trypanosome non-specific IgM antibodies detected in serum of Trypanosoma congolense-infected cattle are polyreactive |
| title_short | Trypanosome non-specific IgM antibodies detected in serum of Trypanosoma congolense-infected cattle are polyreactive |
| title_sort | trypanosome non specific igm antibodies detected in serum of trypanosoma congolense infected cattle are polyreactive |
| topic | trypanosoma congolense antibodies serum trypanosomiasis cattle antigens |
| url | https://hdl.handle.net/10568/33222 |
| work_keys_str_mv | AT buzaj trypanosomenonspecificigmantibodiesdetectedinserumoftrypanosomacongolenseinfectedcattlearepolyreactive AT naessensjan trypanosomenonspecificigmantibodiesdetectedinserumoftrypanosomacongolenseinfectedcattlearepolyreactive |