Functional expression of a bovine major histocompatibility complex class I gene in transgenic mice
Major histocompatibility complex (MHC) class I restricted cellular immune responses play an important role in immunity to intracellular pathogens. By binding antigenic peptides and presenting them to T cells, class I molecules impose significant selection on the targets of immune responses. Candidat...
| Autores principales: | , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2002
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/33220 |
| _version_ | 1855527267308929024 |
|---|---|
| author | Russell, G.C. Oliver, R.A. Craigmile, S. Nene, Vishvanath M. Glass, E.J. |
| author_browse | Craigmile, S. Glass, E.J. Nene, Vishvanath M. Oliver, R.A. Russell, G.C. |
| author_facet | Russell, G.C. Oliver, R.A. Craigmile, S. Nene, Vishvanath M. Glass, E.J. |
| author_sort | Russell, G.C. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Major histocompatibility complex (MHC) class I restricted cellular immune responses play an important role in immunity to intracellular pathogens. By binding antigenic peptides and presenting them to T cells, class I molecules impose significant selection on the targets of immune responses. Candidate vaccine antigens for cellular immune responses should therefore be analysed in the context of MHC class I antigen presentation. Transgenic mice expressing human MHC (HLA) genes provide a useful model for the identification of potential cytotoxic T lymphocyte (CTL) antigens. To facilitate the analysis of candidate CTL vaccines in cattle, we have produced transgenic mice expressing a common bovine MHC (BoLA) class I allele. The functional BoLA-A11 gene, carried on a 7 kb genomic DNA fragment, was used to make transgenic mice by pronuclear microinjection. Three transgenic mouse lines carrying the BoLA-A11 gene were established. Expression of the BoLA-A11 gene was found in RNA and the A11 product could be detected on the surface of spleen and blood cells. Functional analysis of the A11 transgene product, and its ability to act as an antigen presenting molecules in the mouse host will be discussed. |
| format | Journal Article |
| id | CGSpace33220 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2002 |
| publishDateRange | 2002 |
| publishDateSort | 2002 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace332202024-05-01T08:17:42Z Functional expression of a bovine major histocompatibility complex class I gene in transgenic mice Russell, G.C. Oliver, R.A. Craigmile, S. Nene, Vishvanath M. Glass, E.J. cattle major histocompatibility complex mice gene transfer immune response antigens Major histocompatibility complex (MHC) class I restricted cellular immune responses play an important role in immunity to intracellular pathogens. By binding antigenic peptides and presenting them to T cells, class I molecules impose significant selection on the targets of immune responses. Candidate vaccine antigens for cellular immune responses should therefore be analysed in the context of MHC class I antigen presentation. Transgenic mice expressing human MHC (HLA) genes provide a useful model for the identification of potential cytotoxic T lymphocyte (CTL) antigens. To facilitate the analysis of candidate CTL vaccines in cattle, we have produced transgenic mice expressing a common bovine MHC (BoLA) class I allele. The functional BoLA-A11 gene, carried on a 7 kb genomic DNA fragment, was used to make transgenic mice by pronuclear microinjection. Three transgenic mouse lines carrying the BoLA-A11 gene were established. Expression of the BoLA-A11 gene was found in RNA and the A11 product could be detected on the surface of spleen and blood cells. Functional analysis of the A11 transgene product, and its ability to act as an antigen presenting molecules in the mouse host will be discussed. 2002-09 2013-07-03T05:26:15Z 2013-07-03T05:26:15Z Journal Article https://hdl.handle.net/10568/33220 en Limited Access Elsevier Veterinary Immunology and Immunopathology;87(3-4): 417-421 |
| spellingShingle | cattle major histocompatibility complex mice gene transfer immune response antigens Russell, G.C. Oliver, R.A. Craigmile, S. Nene, Vishvanath M. Glass, E.J. Functional expression of a bovine major histocompatibility complex class I gene in transgenic mice |
| title | Functional expression of a bovine major histocompatibility complex class I gene in transgenic mice |
| title_full | Functional expression of a bovine major histocompatibility complex class I gene in transgenic mice |
| title_fullStr | Functional expression of a bovine major histocompatibility complex class I gene in transgenic mice |
| title_full_unstemmed | Functional expression of a bovine major histocompatibility complex class I gene in transgenic mice |
| title_short | Functional expression of a bovine major histocompatibility complex class I gene in transgenic mice |
| title_sort | functional expression of a bovine major histocompatibility complex class i gene in transgenic mice |
| topic | cattle major histocompatibility complex mice gene transfer immune response antigens |
| url | https://hdl.handle.net/10568/33220 |
| work_keys_str_mv | AT russellgc functionalexpressionofabovinemajorhistocompatibilitycomplexclassigeneintransgenicmice AT oliverra functionalexpressionofabovinemajorhistocompatibilitycomplexclassigeneintransgenicmice AT craigmiles functionalexpressionofabovinemajorhistocompatibilitycomplexclassigeneintransgenicmice AT nenevishvanathm functionalexpressionofabovinemajorhistocompatibilitycomplexclassigeneintransgenicmice AT glassej functionalexpressionofabovinemajorhistocompatibilitycomplexclassigeneintransgenicmice |