Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva
Two distinct genes encoding single domain, ATP-binding cassette transport protein homologues of Theileria parva were cloned and sequenced. Neither of the genes is tandemly duplicated. One gene, TpABCI, encodes a predicted protein of 593 amino acids with an N-terminal hydrophobic domain containing si...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2001
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/33101 |
| _version_ | 1855513805414465536 |
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| author | Kibe, M.K. Macklin, M.D. Gobright, E.I. Bishop, Richard P. Urakawa, T. Ole-MoiYoi, O.K. |
| author_browse | Bishop, Richard P. Gobright, E.I. Kibe, M.K. Macklin, M.D. Ole-MoiYoi, O.K. Urakawa, T. |
| author_facet | Kibe, M.K. Macklin, M.D. Gobright, E.I. Bishop, Richard P. Urakawa, T. Ole-MoiYoi, O.K. |
| author_sort | Kibe, M.K. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Two distinct genes encoding single domain, ATP-binding cassette transport protein homologues of Theileria parva were cloned and sequenced. Neither of the genes is tandemly duplicated. One gene, TpABCI, encodes a predicted protein of 593 amino acids with an N-terminal hydrophobic domain containing six potential membrane-spanning segments. A single discontinuous ATP-binding element was located in the C-terminal region of TpABCI. The second gene, TpABC2, also contains a single C-terminal ATP-binding motif. Copies of TpABC2 were present at four loci in the T. parva genome on three different chromosomes. TpABCI exhibited allelic polymorphism between stocks of the parasite. Comparison of cDNA and genomic sequences revealed that TpABCl contained seven short introns, between 29 and 84 bp in length. The full-length TpABCI protein was expressed in insect cells using the baculovirus system. Application of antibodies raised against the recombinant antigen to western blots of T. parva piro-plasm lysates detected an 85 kDa protein in this life-cycle stage. |
| format | Journal Article |
| id | CGSpace33101 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2001 |
| publishDateRange | 2001 |
| publishDateSort | 2001 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace331012023-12-08T19:36:04Z Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva Kibe, M.K. Macklin, M.D. Gobright, E.I. Bishop, Richard P. Urakawa, T. Ole-MoiYoi, O.K. theileria parva genes dna rna immunization chromosomes electrophoresis polymorphism isolation infectious diseases parasitology Two distinct genes encoding single domain, ATP-binding cassette transport protein homologues of Theileria parva were cloned and sequenced. Neither of the genes is tandemly duplicated. One gene, TpABCI, encodes a predicted protein of 593 amino acids with an N-terminal hydrophobic domain containing six potential membrane-spanning segments. A single discontinuous ATP-binding element was located in the C-terminal region of TpABCI. The second gene, TpABC2, also contains a single C-terminal ATP-binding motif. Copies of TpABC2 were present at four loci in the T. parva genome on three different chromosomes. TpABCI exhibited allelic polymorphism between stocks of the parasite. Comparison of cDNA and genomic sequences revealed that TpABCl contained seven short introns, between 29 and 84 bp in length. The full-length TpABCI protein was expressed in insect cells using the baculovirus system. Application of antibodies raised against the recombinant antigen to western blots of T. parva piro-plasm lysates detected an 85 kDa protein in this life-cycle stage. 2001-09-01 2013-07-03T05:26:03Z 2013-07-03T05:26:03Z Journal Article https://hdl.handle.net/10568/33101 en Limited Access Springer Parasitology Research;87: 741-750 |
| spellingShingle | theileria parva genes dna rna immunization chromosomes electrophoresis polymorphism isolation infectious diseases parasitology Kibe, M.K. Macklin, M.D. Gobright, E.I. Bishop, Richard P. Urakawa, T. Ole-MoiYoi, O.K. Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva |
| title | Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva |
| title_full | Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva |
| title_fullStr | Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva |
| title_full_unstemmed | Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva |
| title_short | Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva |
| title_sort | characterisation of single domain atp binding cassette protein homologues of theileria parva |
| topic | theileria parva genes dna rna immunization chromosomes electrophoresis polymorphism isolation infectious diseases parasitology |
| url | https://hdl.handle.net/10568/33101 |
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