Physical and transcriptional organization of the ribosomal RNA genes of the savannah-type Trypanosoma congolense

Ribosomal RNA genes have been cloned from the major species of African trypanosomes. Complete nucleotide sequence composition of the small subunit (SSU) and portions of the large subunit (LSU) ribosomal RNA genes was determined for each of these trypanosome species. In contrast to the situation in T...

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Main Authors: Urakawa, T., Majiwa, Phelix A.O.
Format: Journal Article
Language:Inglés
Published: Springer 2001
Subjects:
Online Access:https://hdl.handle.net/10568/33100
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author Urakawa, T.
Majiwa, Phelix A.O.
author_browse Majiwa, Phelix A.O.
Urakawa, T.
author_facet Urakawa, T.
Majiwa, Phelix A.O.
author_sort Urakawa, T.
collection Repository of Agricultural Research Outputs (CGSpace)
description Ribosomal RNA genes have been cloned from the major species of African trypanosomes. Complete nucleotide sequence composition of the small subunit (SSU) and portions of the large subunit (LSU) ribosomal RNA genes was determined for each of these trypanosome species. In contrast to the situation in Trypanosoma brucei, in savannah-type T. congolense the LSU ribosomal RNA is cleaved twice, to generate two additional prominent fragments. This leads to the different profiles observed when the rRNA molecules from these two trypanosome species are resolved in agarose gels. From the nucleotide sequences of the 18S RNA, a phylogenetic tree was derived depicting the relationships among the T. congolense complex of trypanosomes and the other species of trypanosomes.
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spelling CGSpace331002024-05-01T08:19:27Z Physical and transcriptional organization of the ribosomal RNA genes of the savannah-type Trypanosoma congolense Urakawa, T. Majiwa, Phelix A.O. trypanosoma congolense rna genes dna hybridization Ribosomal RNA genes have been cloned from the major species of African trypanosomes. Complete nucleotide sequence composition of the small subunit (SSU) and portions of the large subunit (LSU) ribosomal RNA genes was determined for each of these trypanosome species. In contrast to the situation in Trypanosoma brucei, in savannah-type T. congolense the LSU ribosomal RNA is cleaved twice, to generate two additional prominent fragments. This leads to the different profiles observed when the rRNA molecules from these two trypanosome species are resolved in agarose gels. From the nucleotide sequences of the 18S RNA, a phylogenetic tree was derived depicting the relationships among the T. congolense complex of trypanosomes and the other species of trypanosomes. 2001-05-18 2013-07-03T05:26:03Z 2013-07-03T05:26:03Z Journal Article https://hdl.handle.net/10568/33100 en Limited Access Springer Parasitology Research;87: 431-438
spellingShingle trypanosoma congolense
rna
genes
dna
hybridization
Urakawa, T.
Majiwa, Phelix A.O.
Physical and transcriptional organization of the ribosomal RNA genes of the savannah-type Trypanosoma congolense
title Physical and transcriptional organization of the ribosomal RNA genes of the savannah-type Trypanosoma congolense
title_full Physical and transcriptional organization of the ribosomal RNA genes of the savannah-type Trypanosoma congolense
title_fullStr Physical and transcriptional organization of the ribosomal RNA genes of the savannah-type Trypanosoma congolense
title_full_unstemmed Physical and transcriptional organization of the ribosomal RNA genes of the savannah-type Trypanosoma congolense
title_short Physical and transcriptional organization of the ribosomal RNA genes of the savannah-type Trypanosoma congolense
title_sort physical and transcriptional organization of the ribosomal rna genes of the savannah type trypanosoma congolense
topic trypanosoma congolense
rna
genes
dna
hybridization
url https://hdl.handle.net/10568/33100
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AT majiwaphelixao physicalandtranscriptionalorganizationoftheribosomalrnagenesofthesavannahtypetrypanosomacongolense