An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule
Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p150)...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
1998
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/33099 |
| _version_ | 1855527306201661440 |
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| author | Katende, J. Morzaria, S.P. Toye, Philip G. Skilton, Robert A. Nene, Vishvanath M. Nkonge, C. Musoke, A.J. |
| author_browse | Katende, J. Morzaria, S.P. Musoke, A.J. Nene, Vishvanath M. Nkonge, C. Skilton, Robert A. Toye, Philip G. |
| author_facet | Katende, J. Morzaria, S.P. Toye, Philip G. Skilton, Robert A. Nene, Vishvanath M. Nkonge, C. Musoke, A.J. |
| author_sort | Katende, J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p150) were selected for a more detailed anlysis. The p67 and p104 antigens were present only in the sporozoite lysates, whereas PIM and p150 were found in both sporozoite and schizont lysates. The four antigens were expressed as recombinant fusion proteins and were compared with each other in an enzyme-linked immunosorbent assay (ELISA) and in the whole-schizont-based indirect fluorescent antibody test (IFAT) in terms of their ability to detect antibodies in sera of experimentally infected cattle. The PIM-based ELISA provided a higher degree of sensitivity and specificity than did the ELISA using the other three recombinant antigens or the IFAT. Further evaluation of the PIM-ELISA using experimental sera derived from cattle infected with different hemoparasites and field sera from endemic and nonendemic T. parva areas showed that the assay had a sensitivity of > 99 percent and a specificity of between 94 percent and 98 percent. |
| format | Journal Article |
| id | CGSpace33099 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1998 |
| publishDateRange | 1998 |
| publishDateSort | 1998 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace330992024-01-17T12:58:34Z An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule Katende, J. Morzaria, S.P. Toye, Philip G. Skilton, Robert A. Nene, Vishvanath M. Nkonge, C. Musoke, A.J. theileria parva radioimmunoassay antibodies cattle polymorphism Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p150) were selected for a more detailed anlysis. The p67 and p104 antigens were present only in the sporozoite lysates, whereas PIM and p150 were found in both sporozoite and schizont lysates. The four antigens were expressed as recombinant fusion proteins and were compared with each other in an enzyme-linked immunosorbent assay (ELISA) and in the whole-schizont-based indirect fluorescent antibody test (IFAT) in terms of their ability to detect antibodies in sera of experimentally infected cattle. The PIM-based ELISA provided a higher degree of sensitivity and specificity than did the ELISA using the other three recombinant antigens or the IFAT. Further evaluation of the PIM-ELISA using experimental sera derived from cattle infected with different hemoparasites and field sera from endemic and nonendemic T. parva areas showed that the assay had a sensitivity of > 99 percent and a specificity of between 94 percent and 98 percent. 1998-04 2013-07-03T05:26:03Z 2013-07-03T05:26:03Z Journal Article https://hdl.handle.net/10568/33099 en Open Access Springer Parasitology Research;84(5): 408-416 |
| spellingShingle | theileria parva radioimmunoassay antibodies cattle polymorphism Katende, J. Morzaria, S.P. Toye, Philip G. Skilton, Robert A. Nene, Vishvanath M. Nkonge, C. Musoke, A.J. An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule |
| title | An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule |
| title_full | An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule |
| title_fullStr | An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule |
| title_full_unstemmed | An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule |
| title_short | An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule |
| title_sort | enzyme linked immunosorbent assay for detection of theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule |
| topic | theileria parva radioimmunoassay antibodies cattle polymorphism |
| url | https://hdl.handle.net/10568/33099 |
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